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ProteomicsHigh-throughput and high-sensitivity phosphoproteomics with the EasyPhos platform
Mass spectrometry has transformed the field of cell signalling by enabling global studies of dynamic protein phosphorylation (‘phosphoproteomics’). Recent developments are enabling increasingly sophisticated phosphoproteomics studies, but practical challenges remain. The EasyPhos workflow addres...
ORGANISM(S): Homo sapiens (Human) 
2018-09-07 | PXD009227 | Pride
Glioblastoma Cancer Proteomics Post-translational modification Signal transduction Mass spectrometry Phosphorylation Egf Phosphoproteomics 
ProteomicsPhosphoproteomics sample preparation impacts biological interpretation of signalling responses
Quantitative data-dependent acquisition phosphoproteomics (DDAP) has revolutionised cell signaling studies. However, the impact of the specific DDAP sample preparation method on the phosphoproteome quantified is unclear as each study quantifies only a fraction of the phosphoproteome. Here, we compar...
ORGANISM(S): Mus musculus (Mouse) 
2021-12-25 | PXD025376 | Pride
Phosphoproteomics Tio2 Mouse High-throughput phosphoproteomics Scxphos Sample preparation Highphos Comparative analysis 
ProteomicsPhosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media
Cardiac maturation is an important developmental phase where there are profound biological and functional changes after birth in mammals. Herein, we use our profiling of human heart maturation in vivo to identify key drivers of maturation in our human cardiac organoid (hCO) model. After screening of...
ORGANISM(S): Homo sapiens (Human) 
2025-04-17 | PXD054810 | Pride
Cardiac organoids Maturation Phosphorylation Pacing 
ProteomicsAPEX1 alters the phospho-landscape of SKOV3 cells
APEX1 overexpression and knockdown cell lines were established based on SKOV3 cell line. Lable-free quantitative phosphoproteomics was done to evaluate the impact of APEX1 on cellular phosphoproteomics
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD040041 | Pride
Lc-msms  phosphoproteomics 
ProteomicsLabel-free global phosphoproteomics experiment using PI3K-AKT-mTOR-S6K and RAF-MEK-ERK-RSK pathway activation and inhibition
The signaling network of skeletal muscle cells is controlled by a variety of protein kinases. Although many kinases are known players, their downstream targets are still largely unexplored. To gain further knowledge about the PI3K-AKT-mTOR-S6K and the RAF-MEK-ERK-RSK signaling networks in myotubes, ...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-22 | PXD029678 | Pride
Phosphoproteomics Mouse Akt Rsk Ms S6k Skeletal muscle 
ProteomicsBiochemical characterisation of Mer3 helicase interactions and the protection of meiotic recombination intermediates
Crossing over between homologs is critical for the stable segregation of chromosomes during the first meiotic division. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of the majority of crossovers during meiosis. Here we...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-07-20 | PXD039839 | Pride
Crossover Yeast Phospho proteomics Meiosis Mer3 
ProteomicsValidation of known and novel AKT, S6K and RSK targets by targeted MS
Based on the global SILAC and label-free phosphoproteomics experiments, we generated an inclusion list for validation of known and novel AKT, RSK and S6K targets containing the RxRxxp[ST] motif. Samples were analyzed by targeted MS using PRM.
ORGANISM(S): Mus musculus (Mouse) 
2023-02-22 | PXD029633 | Pride
Phosphoproteomics Mouse Akt Rsk Targeted ms S6k Skeletal muscle 
ProteomicsMaresin-1 and Resolvin E1 promote regenerative properties of periodontal ligament stem cells under inflammatory conditions
Maresin-1 (MaR1) and Resolvin E1 (RvE1) are specialized pro-resolving lipid mediators (SPMs) that regulate inflammatory processes. We have previously demonstrated the hard and soft tissue regenerative capacity of RvE1 in an in vivo model of periodontal disease characterized by inflammatory tissue de...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019546 | Pride
Maresin Phosphoproteomics Stem cells Resolvin e1 
ProteomicsProteomic and phosphoproteomic of C2C12 myogenesis and MAPK inhibition
Myogenesis is governed by signalling networks whose regulations are tightly controlled in a time-dependent manner. While different protein kinases have been identified to regulate various aspects of myogenesis, knowledge on the global signalling networks and their downstream substrates during myogen...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-31 | PXD028713 | Pride
C2c12 Mapk Myogenesis 
ProteomicsGalectin-1 Modulates Cell Adhesions, Caveolae, and Vascular Permeability in Kidney Endothelial Cells - Insights from Phosphoproteomics
Microvascular inflammation and endothelial injury, triggered by interferon-gamma (IFNgamma), are hallmarks of antibody-mediated rejection (ABMR), the leading cause of premature kidney allograft loss. Chronic ABMR exhibits glomerular basement membrane and extracellular matrix (ECM) remodeling and end...
ORGANISM(S): Homo sapiens (Human) 
2026-10-15 | PXD074969 | Pride
Phosphoproteomics Tio2 enrichment In vitro Transplant Antibody mediated rejection Galectin-1 Msstatsptm Easyphos protocol Primary human glomerular microvascular endothelial cells Sirna knockdown Interferon-gamma 
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