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ProteomicsEffect of truncation mutants of the trypanosomatid nuclear lamina protein NUP-1 on the whole cell proteome in T. brucei
The nuclear lamina has multiple functions, including maintaining nuclear structural integrity and differential gene expression. Correct spatial and temporal lamina assembly is essential to meet these and other roles. Recently, it emerged that multiple lamina systems exist that are likely products of...
ORGANISM(S): Trypanosoma brucei brucei TREU927 
2021-06-03 | PXD019978 | Pride
Lamina Trypanosoma Nuclear organisation Molecular assembly Heterochromatin 
ModelsPeterson2010 - Integrated calcium homeostasis and bone remodelling
Peterson2010 - integrated calcium homeostasis and bone remodelling This model is described in the article: A physiologically based mathematical model of integrated calcium homeostasis and bone remodeling. Peterson MC, Riggs MM. Bone 2010 Jan; 46(1): 49-63 Abstract: ...
2024-09-02 | BIOMD0000000613 | BioModels
ProteomicsCharacterisation of cullin E3 ubiquitin ligases in trypanosomes
Trypanosomes have seven cullin paralogs, the majority lineage-specific. Six of those cullins (TbCul-A, TbCul-C, TbCul-D TbCul-E, TbCul-F and TbCul-G) were endogenously 3xHA-epitope tagged at the C-terminus. We used affinity capture/mass spectrometry to determine the composition of these cullin compl...
ORGANISM(S): Trypanosoma brucei 
2024-03-25 | PXD042022 | Pride
Cullin Evolution Affinity isolation Protein turnover Ubiquitin Cryomilling E3 ligase 
MetabolomicsMetabolic Dynamics in Escherichia coli-Based Cell-Free Systems
The field of metabolic engineering has yielded remarkable accomplishments in using cells to produce valuable molecules, and cell-free expression (CFE) systems have the potential to push the field even further. However, CFE systems still face some outstanding challenges, including endogenous metaboli...
2023-02-22 | MTBLS2630 | MetaboLights
ProteomicsWhole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells
A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we ...
ORGANISM(S): Trypanosoma brucei 
2023-03-11 | PXD031887 | Pride
Xenobiotics Drug metabolism Drug accumulation Trypanosome Crispr/cas9 Invariant surface glycoprotein Suramin 
ProteomicsInteractome of the two T. brucei Poly(A) binding protein paralogs PABP1and PABP2
Poly(A) binding proteins (PABPs) regulate mRNA fate by controlling mRNA stability and translation through interactions with both the poly(A) tail and eIF4F complex. Here we identified a protein interaction cohort for both T. brucei PABPs by cryo-mill/affinity purification-mass spectrometry.
ORGANISM(S): Trypanosoma brucei 
2018-07-16 | PXD008839 | Pride
Pabp1 Pabp2 Poly(a) binding Trypanosoma brucei Mrna stability Interactome 
ProteomicsTrypannosome brucei brucei 427 TbTPR86 pullout
TPR86 is a tetra tricopeptide repeat containing protein, which we detected by the pull out experiment of TbSkp-Z, which involves in the regulation of surface proteome changes and suramin seisitivity. In this previous experiments, a deubiquitylase, USP7 was detected as a complex member with strong af...
ORGANISM(S): Trypanosoma brucei 
2025-05-06 | PXD021797 | Pride
Pulldown Skp1 Pullout Tpr Brucei Trypanosome Usp7 
ProteomicsTrypanosoma brucei global proteome changes upon suramin exposure
To understand how suramin kills Trypanosoma brucei, we examined the impact on proteome at different timepoints
ORGANISM(S): Trypanosoma brucei 
2020-04-30 | PXD014215 | Pride
Trypanosoma brucei Drug mode of action Suramin 
ProteomicsComparative proteomic analysis between light and dark-adapted Euglena gracilis
We sequenced and assembled a detailed transcriptome and draft genome for E. gracilis Z1. To improve annotation and investigate gene expression in E. gracilis, where most transcripts are also trans-spliced, we conducted comparative proteomic and transcriptomic analysis between light and dark-adapted ...
ORGANISM(S): Euglena gracilis 
2019-01-14 | PXD009998 | Pride
Euglena gracilis Photosynthesis Plastid Light dark adaption 
GenomicsMutational patterns and regulatory networks in epigenetic subgroups of meningioma (H033)
DNA methylation patterns delineate clinically relevant subgroups of meningioma. We previously established the six meningioma methylation classes (MC) benign-1, 2, 3, intermediate-A, B and malignant. Here, we set out to identify subgroup-specific mutational patterns and pathway regulation. Whole-gen...
EGAS00001003481 | EGA
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