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ProteomicsTick (Ixodes ricinus) salivary antigens by LC-MS/MS
Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the sal...
ORGANISM(S): Homo sapiens (Human) Ixodes ricinus 
2021-09-09 | PXD021370 | Pride
Ticks Antigen Saliva Immunoprecipitation 
ProteomicsIdentification of proteins in proximity to VAP and VPS13L1 in P. falciparum blood stages
P. falciparum blood stage parasites maintain an intricate system of membranes, requiring precise mechanisms of lipid synthesis and trafficking. Lipid transfer proteins (LTPs) at interorganelle membrane contact sites (MCSs) have emerged as key for ensuring proper lipid distribution in cells but so fa...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2025-07-28 | PXD066159 | Pride
Malaria Membrane contact sites Erythrocytic stages Lipid transfer proteins Bioid 
ProteomicsMimic miR-497a-5p in vivo intervention
The use of microRNA mimics to increase microRNA levels in the developing organs of newborn mice. Livers from mice treated with 100 mg/kg of an in vivo miR-497a-5p LNA mimic at postnatal day (P)1 and P3 were harvested at postnatal day (P)5. Lungs and livers from mice treated with daily 10 mg/kg in...
ORGANISM(S): Mus musculus (Mouse) 
2026-07-13 | PXD053069 | Pride
Microrna Development In vivo mimic 
UnknownTrifunctional lipid probes for comprehensive studies of single lipid species in living cells.
Not available
S-EPMC5320957 | biostudies-literature
ProteomicsPreparation of tirapazamine-resistant hepatoma cell line
The aim of the project was to obtain murine hepatoma MH22a [1] cell subline, resistant to anticancer agent 3-amino-1,2,4-benzotriazine-1,4-dioxide (tirapazamine, TPZ), and examine the changes of the protein expression as compared with parental cells, with the main emphasis on the changes of redox pr...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD039876 | Pride
Tirapazamine  hepatoma  resistance 
UnknownMemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
Not available
S-SCDT-10_1038-S44318-024-00063-Y | biostudies-other
ProteomicsProtein interaction partners of drosophila Unr identified by pull down of wild type and an interdomain mutant protein
To identify an RNP interactome of Unr, V5 tagged wildtype protein and a mutant containing several interdomain contacts, that shows reduced protein expression were tansfected in SL2 cells after a siRNA knoch-down of the endogenous protein levels. After pulling down the transfected protein with an ant...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2020-07-14 | PXD018115 | Pride
Unr Rnp Pull-down Interactome Rbp 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-04-08 | PXD015858 | Pride
Lipid Mass spectrometry Interactors Streptavidin Membrane proteins Chip-sicap Biotinylation Bioid Chip Ceramide-binding proteins 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-27 | PXD016576 | Pride
Streptavidin Affinity purification Mass spectrometry Dna pull down Chip sicap Lipid binding protein Bioid Membrane protein Chip Biotin 
ProteomicsIdentification of proteins crosslinked to lysosomal sphingosine and cholesterol probes
HeLa cells were labelled with lysosomally localized photocrosslinkable and clickable probes for sphingosine and cholesterol. Upon photocrosslinking and cell lysis, the protein-lipid complexes were clicked to biotin azide and enriched via Streptavidin-immunoprecipitation. Enriched protein-lipid compl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-07 | PXD039780 | Pride
Lysosomal lipids Photoaffinity labelling Click chemistry 
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