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ProteomicsFatty-acylation in RAW264.7 - Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins
Dietary unsaturated fatty acids beneficially affect human health, in part by modulating the immune system, but the mechanism is not completely understood. Given that unsaturated fatty acids have been shown to be covalently incorporated into a small subset of proteins, we designed three alkyne-tagged...
ORGANISM(S): Mus musculus (Mouse) 
2018-10-26 | PXD003545 | Pride
Lc-msms Raw264.7 Unsaturated fatty acid Fatty-acylation 
ProteomicsIdentification of bile acid-interacting proteins in Salmonella Typhimurium (14028S) by chemoproteomics
The microbiota generates structurally diverse small molecules that can regulate host physiology and disease. Of these microbiota metabolites, bile acids have emerged as important modulators of host immunity and microbial pathogenesis. While the modes of action for different bile acids on host pathwa...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028 
2022-09-29 | PXD034373 | Pride
Photoaffinity Bile acids Chemoproteomics Salmonella 
ProteomicsDirect analysis of IFITM3 S-fatty-acylation, part 2
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and anothe...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008401 | Pride
Ifitm3 S-fatty-acylation Site identification S-palmitoylation 
ProteomicsDirect analysis of IFITM3 S-fatty-acylation, part 1
Given our laboratory interest in IFITM3 S-fatty-acylation and antiviral activity, we sought to directly characterize fatty acids that are covalently attached to the Cys residues of IFITM3. IFITM3 comprises two S-fatty-acylation sites (C71 and C72) in proximity of a intramembrane domain, and another ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008400 | Pride
Post translational modifications Modification site identification S-fatty-acylation Ifitm3 S-palmitoylation 
ProteomicsDirect identification of protein S-palmitoylation sites
S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryot...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008399 | Pride
S-palmitoylation Site identification S-fatty-acylation 
ProteomicsS-acylated and S-palmitoylated proteins in HeLa
S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryot...
ORGANISM(S): Homo sapiens (Human) 
2018-03-28 | PXD008398 | Pride
Hela Label free quatification S-acylation Acyl-rac S-palmitoylation Alk-16 chemical reporter Fatty acylation Lc-ms/ms 
ProteomicsS-acylation and fatty acylation in Naïve and LPS/IFN- gamma activated Raw264.7
The advances in chemical proteomics have significantly expanded our understanding of the diversity and abundance of fatty-acylated proteins in eukaryotes, and reveal novel functions for these lipid protein modifications. Nonetheless, quantitative comparative proteomic analysis of fatty-acylated prot...
ORGANISM(S): Mus musculus (Mouse) 
2018-03-28 | PXD003652 | Pride
Raw264.7 Post translational modifications S-acylation Fatty acylation S-palmitoylation 
ProteomicsChemoproteomics of microbiota-derived indole metabolites in mammalian cells
The microbiota generates diverse metabolites to modulate host physiology and disease, but their protein targets and mechanisms of action have not been fully elucidated. To address this challenge, we focused on microbiota-derived indole metabolites and develop photoaffinity chemical probes for proteo...
ORGANISM(S): Homo sapiens (Human) 
2023-05-31 | PXD040163 | Pride
Bacterial metabolites Mammalian cells Chemical proteomics Photoaffinity probes 
ProteomicsNuclear receptor chemical reporter enables domain-specific analysis of ligands in mammalian cells
The characterization of specific metabolite–protein interactions is important in chemical biology and drug discovery. For example, nuclear receptors (NRs) are a family of ligand-activated transcription factors that regulate diverse physiological processes in animals and are key targets for therapeut...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD020969 | Pride
Lc-msms 
ProteomicsPeptidoglycan metabolite photoaffinity reporters reveal direct binding to intracellular pattern recognition receptors and Arf GTPases
Extensive structure-activity studies of iE-DAP and MDP have demonstrated their selective activation of NOD1- and NOD2-expressing cells, respectively. Nonetheless, the direct binding of iE-DAP and MDP to NOD1 and NOD2, respectively, as well as other proteins in mammalian cells has not been systematic...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD012350 | Pride
Mdp Gel-based proteomics Nod2 Hek293t 
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