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ProteomicsInteractome of alanine:glyoxylate aminotransferase polimorphic forms
This dataset includes raw data on the interactome of two polymorphic forms of alanine:glyoxylate aminotransferase (AGT), the major allele, AGT-Ma and the minor one AGT-Mi. Theproteins were expressed in HEK293 cells and the interactome was analysed using co-immunoprecipitation mass spectrometry (IP-M...
ORGANISM(S): Homo sapiens (Human) 
2022-05-04 | PXD025661 | Pride
Alanine:glyoxylate aminotransferase Human Hek293 Interactome 
ProteomicsProtein binder based IP-MS of SLC12A6 (KCC3) in Hek293WT OE cell lines
Specific protein binders with high affinity, such as antibodies or nanobodies, are scarce for most solute carrier (SLCs) transporters. Moreover, general approaches that can systematically generate binders for multi-transmembrane proteins, such as SLCs, channels or GPCRs, are still missing. Purifying...
ORGANISM(S): Homo sapiens (Human) 
2024-06-17 | PXD046995 | Pride
Human Ip-ms Slc12a6 (kcc3) Protein binders 
ProteomicsIdentification of MAST1 interactors in HEK293 cells
MAST1 was N-terminally EGFP-tagged and overexpressed in HEK293 cells. Various constructs of MAST1, including WT, S90A, S161A, S90A S161A, and N-terminal truncations spanning S90 and S161 were compared in a pairwise manner to identify specific interactors of MAST1. EGFP-MAST1 was immunoprecipitated u...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-09-02 | PXD063727 | Pride
Mast1 Ip-ms Hek293 
ProteomicsProteomics and IP/MS of HCMV US30 in HEK293 cells
Ectopic expression of HCMV US30 in HEK293 cells to examine the host protein alteration and interactors
ORGANISM(S): Homo Sapiens 
2026-04-12 | PXD077060 |
ProteomicsLC-MS/MS analysis: coIP of C9ORF78-Flag from nuclear extracts of HEK293 cells
Interactome analysis of human Flag-tagged C9ORF78, C9ORF78 mutant (R41A) and background control. The coIP's (n=3) were performed from nucelar extract of HEK293 cells followed by LC-ESI-MS/MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-07 | PXD031482 | Pride
Hek293 cells 
ProteomicsIP of endogenous SUMO2/3 in HEK293 cells
Aim of the experiments was to identify endogenously SUMO2/3 modified proteins in HEK293 cells. Cells were treated with dexamethasone (dex) or vehicle (etoh) and with heatshock (+43C, HS) or normal growth temperature (+37C, non-HS). Cells were induced with tetracycline to express BirA*-GR (glucocorti...
ORGANISM(S): Homo sapiens (Human) 
2021-01-11 | PXD017714 | Pride
Sumo2/3 Heatshock Immunoprecipitation Hek293 
ProteomicsImmunoprecipitation/Pull-down of ATP6AP1-FLAG in HEK293 WT cells treated with and without a mitochondrial translation inhibitor
Dataset: HEK293 WT cells were transfected with ATP6AP1-FLAG expression vector for 96h, with or without the presence of 15uM actinonin, a mitochondrial translation inhibitor. Immunoprecipitation was performed using FLAG antibody and Protein-G Sepharose, and the samples were analysed with label-free w...
ORGANISM(S): Homo sapiens (Human) 
2025-11-20 | PXD057148 | Pride
Atp6ap1/ac45 Crispr/cas9 Mitochondrial translation Organellar cross-talk V-atpase Proteostasis Mitochondrial disease Retrograde signalling 
ProteomicsProtein interactions of a novel tRNA-derived small RNA
Explore protein binding partners of novel tRNA (tRNA-Asp-GTC-3'tDR) in HEK293 cells via IP followed by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2025-08-08 | PXD052235 | Pride
Mass spectrometry Protein binding Trna Human Hek293 
GenomicsHomo sapiens
Protein-protein interactome of NSD2 isoforms in HEK293
PRJNA623989 | ENA
ProteomicsAnalysis of UBASH3B interactome in A549 and in HEK293 Flpin cell lines
Determination of the interaction partners of endogenous UBASH3B in A549 cells (PRM) and validation of the interactome after purification of Strep C- and N-terminally tagged UBASH3B in HEK293 FlpIn cell line (DDA).
ORGANISM(S): Homo sapiens (Human) 
2022-10-13 | PXD019878 | Pride
Prm Ubash3b Apms Dda 
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