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MetabolomicsImproving global feature detectabilities through scan range splitting for untargeted metabolomics by high-performance liquid chromatography-Orbitrap mass spectrometry
Untargeted metabolomics aims at obtaining quantitative information on the highest possible number of low-molecular biomolecules present in a biological sample. Rather small changes in mass spectrometric spectrum acquisition parameters may have a significant influence on the detectabilities of metabo...
2016-06-09 | MTBLS233 | MetaboLights
UnknownAptamers as quality control tool for production, storage and biosimilarity of the anti-CD20 biopharmaceutical rituximab.
Not available
S-EPMC6358617 | biostudies-literature
ProteomicsTowards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS
Advanced analytical strategies including top-down and middle-up HPLC-MS approaches have become powerful alternatives to classical bottom-up analysis for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up analysis of polyclonal IgGs posing additional c...
ORGANISM(S): Mus musculus (Mouse) 
2020-10-26 | PXD014710 | Pride
Speb Phl p 6 vaccination Murine igg subclass quantification N-glycosylation 
ProteomicsDilute-and-Shoot Analysis of Therapeutic Monoclonal Antibody Variants in Fermentation Broth: A Method Capability Study
Monoclonal antibodies (mAbs) are widely applied as highly specific and efficient thera-peutic agents for various diseases, including cancer, inflammatory and autoimmune diseases. As protein production in cellular systems inherently generates a multitude of molecular variants, manufacturing of mAbs r...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD011017 | Pride
Glycation Monoclonal antibodies Process analytical technology Electrospray ionization mass spectrometry Pat Glycosylation Biosimilar Mam Multiple attribute monitoring Antibody subunits Structural analysis Fermentation Process monitoring High-performance liquid chromatography 
UnknownTowards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS.
Not available
S-EPMC7581757 | biostudies-literature
ProteomicsA Generic HPLC Method for Absolute Quantification of Oxidation in Monoclonal Antibodies and Fc-Fusion Proteins Using UV and MS Detection
Absolute quantification of methionine oxidation in the Fc domain of biopharmaceuticals. Wedeveloped a middle-down approach employing the cysteine protease IdeS under reducing conditions to obtain three mAb subunits of approximately 25 kDa: Fc/2, Fd’ and LC. These subunits were separated by ion-pair ...
ORGANISM(S): Homo sapiens (Human) 
2018-04-05 | PXD006873 | Pride
Ion-pair reversed-phase Q exactive Ides 
ProteomicsMultiomics assessment in Enchytraeus crypticus exposed to Ag (nano)materials (Ag NM300K and AgNO 3 )
Silver nanomaterials (AgNMs) are broadly used in many products and also rate among the most studied nanoscaled materials. Their ecotoxicological impact in soil invertebrates has been covered, mostly using standard testing, where endpoints like survival and reproduction are assessed. The underlying m...
ORGANISM(S): Enchytraeus crypticus 
2021-09-10 | PXD024444 | Pride
Nanomaterial Lc-ms/ms E. crypticus Ag Ecotoxicology 
MetabolomicsMetabolome analysis via an HPLC-ESI-MS-based experimental and computational pipeline for chronic nephron toxicity profiling
Untargeted metabolomics has the potential to improve the predictivity of in vitro toxicity models and therefore may aid the reduction of expensive and laborious animal models. Here we describe a chronic nephrotoxicity study conducted on a human renal proximal tubular epithelial cell line (RPTEC/TERT...
2015-09-07 | MTBLS140 | MetaboLights
ProteomicsProteins and Molecular Pathways Relevant for Stemness Properties of Tumor-Initiating Pancreatic Cancer Stem Cells Recognized by Differential Proteome-, Gene Expression-, and Functional Analysis
Cancer stem cells (CSCs), a small subset of the tumor bulk with highly malignant properties, are deemed responsible for tumor initiation, growth, metastasis, and relapse. In order to reveal molecular markers and determinants of stemness properties, pancreatic CSCs were enriched in three-dimensional ...
ORGANISM(S): Homo sapiens (Human) 
2020-06-25 | PXD018585 | Pride
Mrp14 Proteomics S100a9 Mac-2-bp Lgals3bp Pancreatic cancer stem cells S100a8 Mrp8 S100 proteins Functional assays Differential proteome analysis Tumor grafting model 
ProteomicsmoDCs HPLC-MS/MS - Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells
To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of known adjuvants coming from pollen, such as lipopolysaccharide (LPS), we performed quantitative proteome analysis of stimulated monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch po...
ORGANISM(S): Homo sapiens (Human) 
2017-12-01 | PXD006196 | Pride
Human Modc Hplc-ms/ms 
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