Human hek293t lc-ms/ms

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Tissue

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Technology Type

Mass Spectrometry (513)
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Mass spectrometry (7)
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SWATH MS (7)
SRM/MRM (5)
DiaPASEF (3)
Mass spectrometry assay (2)
Glycoproteomics (2)
All-ion fragmentation (1)
Mass spectrometry imaging (1)
Proteogenomics (1)

Instrument Platform

LTQ Orbitrap Velos (3)
Liquid Chromatography MS - positive - reverse phase (3)
Liquid Chromatography MS - alternating - hilic (2)
Liquid Chromatography MS - alternating - reverse phase (2)
Liquid Chromatography MS - negative - hilic (2)
Liquid Chromatography MS - positive - hilic (2)
6230B Time-of-Flight LC/MS (1)
LTQ (1)
LTQ Velos (1)
MS:1000031 (1)
MaXis II (1)
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Q Exactive (1)
Q-Tof micro (1)
Liquid Chromatography MS - positive - reverse (1)
Liquid Chromatography MS - negative - reverse (1)
Flow injection analysis MS - positive (1)
Direct infusion MS - positive (1)
Liquid Chromatography MS - (1)
Liquid Chromatography MS - positive - reverse-phase (1)
Liquid Chromatography MS - negative - reverse phase (1)

CHEBI ID

CHEBI:28044 (5)
CHEBI:26271 (4)
CHEBI:17126 (4)
CHEBI:22660 (4)
CHEBI:16811 (4)
CHEBI:28300 (4)
CHEBI:25017 (4)
CHEBI:29016 (4)
CHEBI:15891 (4)
CHEBI:18237 (4)
CHEBI:16449 (4)
CHEBI:18186 (3)
CHEBI:16704 (3)
CHEBI:28867 (3)
CHEBI:16668 (3)
CHEBI:73024 (3)
CHEBI:17562 (3)
CHEBI:16335 (3)
CHEBI:16737 (3)
CHEBI:27897 (3)
CHEBI:15354 (3)
CHEBI:17368 (3)
CHEBI:85574 (2)
CHEBI:67070 (2)
CHEBI:67066 (2)
CHEBI:67067 (2)
CHEBI:67077 (2)
CHEBI:85568 (2)
CHEBI:85567 (2)
CHEBI:85566 (2)

Metabolite Name

Cytidine (3)
Phenylalanine (3)
7-methylguanosine (2)
Hypotaurine (2)
Leucine (2)
Uridine (2)
Pseudouridine (2)
N-acetylglutamate (2)
Inosine (2)
Guanosine (2)
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NAD+ (2)
AMP (2)
1-methyladenosine (2)
CMP (2)
ADP (2)
GMP (2)
Carnitine (2)
Methionine (2)
Choline (2)
CDP (2)
Arginine (2)
Cytosine (2)
Alanine (2)
Proline (2)
Glutamine (2)
ADP-ribose (2)
Hypoxanthine (2)
Lyso-PE 18:1 (1)
Lyso-PC 18:2 (1)

First Public Date

2022 (1)

Modification

15.994915 (3)
2x(15)N labeled L-lysine (2)
4x(15)N labeled L-arginine (2)
57.021464 (2)
-17.026549 (1)
42.010565 (1)
4x(2)H labeled dimethylated residue (1)
6.020129 (1)
71.037114 (1)
79.966331 (1)

Release Date

2015 (3)
2024 (1)
2022 (1)
2021 (1)
2020 (1)
2019 (1)
2013 (1)

Lab affiliation

Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands (9)
Associate Member Developmental Neurobiology St. Jude Children's Research Hospital (8)
Chair of Biochemistry II, Theodor Boveri-Institute, Biocenter, University of Würzburg, Würzburg, Germany (6)
Department of Cell Biology, Harvard Medical School, Boston MA 02115 USA (6)
Department of Cell Biology, Harvard Medical School, Boston, MA 02115 USA (4)
Crick-GSK LinkLabs, UK (3)
DIMEVET Università degli Studi di Milano (3)
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA., Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA (3)
Département de Biologie Cellulaire, Université de Genève (3)
IMol Polish Academy of Sciences, Warsaw, Poland (3)
Immunology Research Center, National Health Research Institutes, Zhunan, Taiwan Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas (3)
Institute of Molecular Health Sciences, Department of Biology, ETH Zürich; Zürich, Switzerland (3)
Medical bioanalytics Institute for ophthalmic research Eberhard-Karls-University Tuebingen (3)
University of California, Riverside (3)
Assistant professor, School of Biological Sciences, Seoul National University (2)
College of Chemistry and Molecular Engineering, Peking University, Beijing, China. (2)
Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, Australia. (2)
Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California (2)
Department of Gene Expression Institute of Molecular Biology and Biotechnology Faculty of Biology Adam Mickiewicz University Uniwersytetu Poznanskiego 6 61-614 Poznan (2)
EMBL Grenoble (2)
GSK (2)
Harvard Medical School (2)
Harvard Medical School Cell Biology Department (2)
Institute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS) (2)
Institute of Biochemistry II, Faculty of Medicine, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany. (2)
Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, Riedberg Campus, Max-von-Laue-Straße 15, 60438 Frankfurt am Main, Germany (2)
Institute of Modern Biology, Nanjing University, Nanjing 210008, Jiangsu, China (2)
Institute of Virology - Viral Immunopathology, Faculty of Medicine, Technical University Munich (2)
Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) (2)
Max Delbruck Center for Molecular Medicine, Robert-Rössle-Str. 10, D-13092 Berlin, Germany (2)

Publication date

2025 (100)
2026 (83)
2024 (82)
2023 (77)
2022 (67)
2021 (47)
2020 (39)
2019 (32)
2018 (11)
2016 (9)
2017 (4)
2013 (2)
2014 (1)

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Flag-GABPα overexpression HEK293T cells LC-MS/MS

Immunoprecipitated by Flag and IgG antibody for protein capture by LC-MS/MS in HEK293T cells infected with GABPα-Flag overexpression plasmid.

ORGANISM(S): Homo sapiens (Human)

2026-02-23 | PXD070466 | Pride

Real-world neonicotinoid mixture exposure reveals synergistic cytotoxicity and divergent pathway vulnerabilities in human cell lines

In this study, five human cell lines (A2780, Chang liver, HEK293T, HeLa, and HepG2) were used as models and exposed to mixtures of neonicotinoids (NEOs) and their metabolites. The mixture concentrations were prepared based on human biomonitoring data to reflect the actual detected levels of NEOs ...

2026-06-22 | MTBLS13896 | MetaboLights

Gain-of-function genetic screens in human cells identify SLC transporters overcoming environmental nutrient restrictions

Solute carrier (SLC) transporters control fluxes of nutrients and metabolites across membranes and thereby represent a critical interface between the microenvironment and cellular and subcellular metabolism. Because of substantial functional overlap, the interplay and relative contributions of SL...

2022-09-20 | MTBLS4268 | MetaboLights

Identification of MELK-mediated Cofilin-1 T70 phosphorylation by IP-LC-MS/MS analysis

To investigate whether MELK phosphorylates Cofilin-1 at residue Threonine 70 (T70), we performed a phosphoproteomic validation using immunoprecipitation coupled with mass spectrometry (IP-LC-MS/MS). HEK293T cells were divided into three experimental groups: (1) MOCK control (Empty Vector), (2) MELK ...

ORGANISM(S): Homo sapiens (Human)

2026-04-01 | PXD074164 | Pride

Cofilin-1 Melk Immunoprecipitation Affinity purification-ms Otssp167 Hek293t T70 phosphorylation Lc-ms/ms

Human LC-MS Data in HEK293T overexpressed LATS2

LC-MS data for Enzyme Activity-Independent NANS Stabilizes LATS2 to Drive Growth and Confer Resistance to Endocrine Therapy and CDK4/6 Inhibitors in HR+/HER2- Breast Cancer

ORGANISM(S): Homo sapiens (Human)

2025-12-14 | PXD065340 | Pride

Human lc-ms lats2

Immunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells

This dataset contains raw mass spectrometry data and associated protein and peptide identification results from anti-Flag immunoprecipitation coupled to LC-MS/MS analysis of Flag and Flag-DJ-1 complexes in HEK293T cells. The dataset was generated to identify candidate DJ-1-associated proteins and is...

ORGANISM(S): Homo sapiens (Human)

2026-07-24 | PXD081552 | Pride

Affinity purification-mass spectrometry Hek293t cells Dj-1 Immunoprecipitation Park7 Protein-protein interactions Lc-ms/ms

The purified SKP2 from HEK293T cells underwent LC-MS analysis.

Flag-tagged SKP2 was purified from HEK293T cells using anti-flag immunoprecipitation and subsequently analyzed via LC-MS to identify its interacting proteins.

ORGANISM(S): Homo sapiens (Human)

2026-09-21 | PXD052608 | Pride

Skp2

Human HEK293T cells LC-MS/MS (Gel-based proteomics)

Fanconi Anemia (FA) pathway is essential for the repair of inter-strand crosslink (ICLs). ICL induces stalled replication forks and triggers activation of FA pathway for efficient ICL repair. Given that stalled replication fork is proximal to ICLs sites, fork-associated proteins may likely coordinat...

ORGANISM(S): Homo sapiens (Human)

2023-05-10 | PXD028246 | Pride

Fanconi anemia pathway Human cells Wdhd1

MKRN3 interacting proteins identified by LC-MS/MS

MKRN3, whose deletion or loss-of-function mutations were genetically associated with human centeral precocious puberty (CPP). To identify the potential substrates for MKRN3, MKRN3 was transfected into HEK293T cells as bait, and its interacting proteins were identified by mass spectrum and functions ...

ORGANISM(S): Homo sapiens (Human)

2021-02-22 | PXD022346 | Pride

Human Mkrn3 Hek293t Lc-ms/ms

Affinity purification of PRPS complex in human cultured cells (HEK293T)- LC-MS/MS

Phosphoribosyl pyrophosphate synthetase (PRPS) is an enzyme conserved across all forms of life, tracing back to the last universal common ancestor (LUCA). PRPS catalyzes the rate-limiting step in converting ribose-5-phosphate (R5P) to phosphoribosyl pyrophosphate (PRPP), a crucial precursor in the b...

ORGANISM(S): Homo sapiens (Human)

2025-05-13 | PXD058828 | Pride

Prps complex Prps1 Prps2 Prpsap2 Lc-ms/ms

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566 Results

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