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ProteomicsPrecise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition
In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2% of this proteome is rapidly degraded...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-09-09 | PXD018794 | Pride
C-terminal to lis1 homology (ctlh) complex Skp/cullin/f-box-containing (scf) complex Ubiquitin ligase Smaug Rna-binding protein 
ProteomicsCross-linking/Mass Spectrometry Combined with Ion Mobility on a timsTOF Pro Instrument for Structural Proteomics
The combination of cross-linking/mass spectrometry (XL-MS) and ion mobility is still underexplored for conducting protein conformational and protein-protein interaction studies. We present a method for analyzing cross-linking mixtures on a timsTOF Pro mass spectrometer that allows separating ions ba...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Escherichia coli 
2022-03-17 | PXD027234 | Pride
Ion mobility Mass spectrometry Chemical cross-linking Protein 3d-structure Protein interaction networks 
ProteomicsConserved hydrophilic checkpoints tune FocA-1 mediated formate:H+ symport
FocA belongs to the widespread, evolutionarily ancient formate-nitrite transporter 30 (FNT) family of pentameric anion channels and translocates formic acid bidirectionally. 31 Here, we identify compartmentalized polarity distribution across the complete FocA 32 pore structure – resolved at 2.56 Å –...
ORGANISM(S): Escherichia coli 
2025-11-10 | PXD054538 | Pride
Foca Xlms Cryo em Formate-nitrite transporter 
ProteomicsMS analysis of SARS-CoV2 proteins from patient samples
We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our metho...
ORGANISM(S): Homo sapiens (Human) 
2020-06-26 | PXD019423 | Pride
Covid-19 Nucleoprotein Sars-cov-2 Mass spectrometry 
ProteomicsRevisiting p53:Sirt1 interaction in the light of controlling p53 acetylation levels
The NAD⁺-dependent deacetylase sirtuin 1 (Sirt1) is known to regulate the tumor suppressor p53 via deacetylation, but the structural basis of the protein-protein interaction between full-length Sirt1 and p53 has so far remained elusive. We apply an integrated approach, combining structural mass spe...
ORGANISM(S): Homo sapiens (Human) 
2026-09-07 | PXD072665 | Pride
Sirtuin 1 Cross-linking Mass spectrometry P53 
ProteomicsExploring GPCR-arrestin interfaces with genetically encoded crosslinkers
-arrestins (arr1 and arr2) are ubiquitous regulators of G protein-coupled receptor (GPCR) signalling. Available data suggest that -arrestins dock to different receptors in different ways. However, the structural characterization of arrestin-GPCR complexes is challenging and alternative approaches to...
ORGANISM(S): Homo sapiens (Human) 
2020-09-21 | PXD020418 | Pride
-arrestins / genetically encoded crosslinkers / g protein-coupled receptor / gpcr-arrestin complexes / live cells 
ProteomicsRAVER1 interconnects lethal EMT and miR/RISC activity by the control of alternative splicing
The RAVER1 protein was proposed to serve as a co-factor in guiding the PTBP-dependent control of alternative splicing (AS). Whether RAVER1 solely acts in concert with PTBPs and how it affects cancer cell fate remained elusive. Here we provide the first comprehensive investigation of RAVER1-contro...
ORGANISM(S): Homo sapiens (Human) 
2024-01-11 | PXD043342 | Pride
Cancer Emt Raver1 Tgfb Microrna Ptbp Alternative splicing Risc 
ProteomicsRUNX1 isoforms in leukemogenesis
Aneuploidy and structural aberrations affecting chromosome 21 (Hsa21) are the most frequent in cytogentic events in acute myeloid leukemia. However, it remains unclear why leukemic blasts select for amplifications of Hsa21 or parts of it and why children with Down syndrome (i.e. trisomy 21) are at a...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD030616 | Pride
Down syndrome Runx1 Acute myeloid leukemia Gata1 Transient abnormal myelopoiesis 
ProteomicsReconstitution of mammalian Cleavage Factor II
Cleavage factor II (CF II) is a poorly characterized component of the multi-protein complex catalyzing 3' cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactio...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD010177 | Pride
Human Rna processing Dsbu 
ProteomicsMS analysis of SARS-CoV2 proteins from patient samples
We developed a simple, MS-based method to specifically detect SARS-CoV-2 proteins from gargle solution samples of COVID-19 patients. Our protocol consists of an acetone precipitation and tryptic digestion of proteins contained within the gargle solution, followed by a targeted MS analysis. Our metho...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-07-16 | MSV000085761 | MassIVE
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