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ProteomicsFe-IMAC column based phospho enrichment
Fe-IMAC columns for robust and reproducible phosphopeptide ernichment, comparison to TiO2 batch and Ti-IMAC tip enrichment, large scale phosphoproteomics coupling Fe-IMAC column pre-enrichment to subsequent hSAX separation
ORGANISM(S): Homo sapiens (Human) 
2015-08-19 | PXD001060 | Pride
Hsax Tio2 Ti-imac Ptm Phospho Fe-imac 
ProteomicsZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple an...
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD018273 | Pride
Affinity enrichment Moac Imac Mass spectrometry Protein phosphorylation Functional proteomics Cell signalling Magnetic particles 
ProteomicsDefeating major contaminants in Fe3+-IMAC phosphopeptide enrichment
Optimized sample preparation workflow for Fe-IMAC phosphopeptide enrichment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-02-19 | PXD008289 | Pride
Human E.coli 
OtherComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Data from ProteomeXchange, PXD ID: PXD001060. File: 00730_A11_P005652_S00_T01_R1.mzml. Published as part of Mol Cell Proteomics. 2015 Jan;14(1):205-15 . From the Abstract: {{i}} ... Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) m...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023168 | GPMDB
Resource Reanalysis 
OtherReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.
Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18 . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application o...
ORGANISM(S): Mus_musculus_viruses, Mouse 
GPM32320003560 | GPMDB
Resource Reanalysis 
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
ProteomicsReproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC
We describe the application of magnetic TiO2 and Ti-IMAC for uniform automated phosphopeptide enrichment. Combining hyper-porous magnetic microspheres with a magnetic particle-handling robot enables rapid (45 minutes), reproducible (r2 = 0.80) and high-fidelity (> 90% purity) phosphopeptide purifica...
ORGANISM(S): Mus musculus (Mouse) 
2014-09-22 | PXD000892 | Pride
Phosphoproteomics automation 
ProteomicsATP-Coated Dual-functionalized Titanium (IV) IMAC Material for Simultaneous Enrichment and Separation of Glycopeptides and Phosphopeptides
Protein glycosylation and phosphorylation are two of the most common post-translational modifications (PTMs), which plays an important role in many biological processes. However, low abundance and poor ionization efficiency of phosphopeptides and glycopeptides make direct MS analysis challenging. Pr...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-07-20 | PXD029775 | Pride
Phosphoproteomics Lc-ms/ms Glycoproteomics 
UnknownComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Not available
S-EPMC4288255 | biostudies-literature
UnknownCotton Ti-IMAC: Developing Phosphorylated Cotton as a Novel Platform for Phosphopeptide Enrichment.
Not available
S-EPMC10730235 | biostudies-literature
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