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ProteomicsNeoantigen targeted dendritic cell vaccine generates durable T cell responses exhibiting the full spectrum of differentiation states in NSCLC patients
Background. Dendritic cell (DC)-based neoantigen vaccination holds potential as a safe and effective adjuvant therapy for patients with early-stage, resectable NSCLC, a tumor type typically characterized by high mutational loads. DCs have the unique ability to elicit robust antitumoral T-cell respon...
ORGANISM(S): Homo sapiens (Human) 
2024-03-17 | PXD044799 | Pride
Cancer Lung non-small cell carcinoma Vaccine Neoantigen Human 
ProteomicsIdentification of SUMO sites by LC-MSMS
We developed a novel method for the identification of SUMO sites by expression of His-tagged SUMO mutants and affinity purification of SUMOylated proteins, followed by trypsin digestion and immunocapture of peptides containing diglycine signature tags. Lab Head: Dr Pascale Cossart, pascale.co...
ORGANISM(S): Homo sapiens (Human) 
2014-07-29 | PXD000459 | Pride
Lc-msms Hela cells Sumo Silac Immunocapture 
ProteomicsProteomics-based identification of ISG15 modification sites during Listeria monocytogenes infection
ISG15 is an interferon-stimulated, ubiquitin-like protein, with anti-viral and anti-bacterial activity, however its precise mechanism of action during viral or bacterial infection remains elusive. We previously found that ISG15 restricts Listeria infection both in vitro and in vivo and identified IS...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-28 | PXD011513 | Pride
Listeria infection Mice Isg15 Ubiquitin-like modification 
ProteomicsProtein profiling upon miR-17-92 induction.
Methionine COFRADIC combined with Arg and Lys SILAC labeling was used to quantify proteins upon induction of miR-17-92 in SHEP neuroblastoma cells. Differently labeled cells treated with tetracycline for 72 h or left untreated, were harvested and mixed lysates were analyzed by methionine COFRADIC to...
ORGANISM(S): Homo sapiens (Human) 
2010-12-10 | PRD000321 | Pride
ProteomicsShort-term remodeling of the host epithelial cell proteome by the listeriolysin O pore-forming toxin
Bacterial pathogens use various strategies to interfere with host cell functions. Among these strategies, bacteria induce transcriptional changes, in order to modify the set of proteins synthetized by the host cell, or target pre-existing proteins by modulating their post-translational modifications...
ORGANISM(S): Homo sapiens (Human) 
2018-05-17 | PXD009339 | Pride
Pore-forming toxin Listeriolysin o Bacterial infection Listeria 
ProteomicsBacterial pore-forming toxins remodel the host epithelial cell proteome
Bacterial pathogens use various strategies to interfere with host cell functions. Among these strategies, bacteria induce transcriptional changes, in order to modify the set of proteins synthetized by the host cell, or target pre-existing proteins by modulating their post-translational modifications...
ORGANISM(S): Homo sapiens (Human) 
2018-11-07 | PXD006996 | Pride
Listeria Infection Listeriolysin o 
ProteomicsBacterial pore-forming toxins remodel the host epithelial cell proteome
Bacterial pathogens use various strategies to interfere with host cell functions. Among these strategies, bacteria induce transcriptional changes, in order to modify the set of proteins synthetized by the host cell, or target pre-existing proteins by modulating their post-translational modifications...
ORGANISM(S): Homo sapiens (Human) 
2018-11-07 | PXD006900 | Pride
Listeria infection Pore-forming toxin Silac Listeriolysin o 
ProteomicsProteomics-based identification of SIRT2 interaction partners
The NAD+ dependent deacetylase enzyme SIRT2 functions in diverse cellular processes including the cell cycle, metabolism and has recently been demonstrated to have important roles during tumorigenesis and bacterial infection. Though predominantly localised in the cytoplasm SIRT2 has been shown to co...
ORGANISM(S): Homo sapiens (Human) 
2020-02-20 | PXD011523 | Pride
Ap-ms Tumorigenesis Bacterial infection Sirt2 Nuclear translocation 
ProteomicsISG15 counteracts Listeria monocytogenes infection
ISG15 is primarily documented as an interferon-stimulated, ubiquitin-like protein (ubl), which has anti-viral activity. Although ISG15 was the founding member of the ubl protein family, very little is known about its function. We have found that ISG15 expression in non-phagocytic cells is dramatic...
ORGANISM(S): Homo sapiens (Human) 
2015-07-24 | PXD001805 | Pride
Interferon Listeria Silac Isg15 
ProteomicsIntegrator restraints paraspeckles assembly by promoting the 3’-end processing of the polyadenylated lncRNA NEAT1_1
Alternative 3’-end RNA processing provides an important source of transcriptome diversification, which impacts various biological processes and the etiology of diseases, including cancer. Here, we focused on a transcript isoform switch that leads to the readthrough expression of the long noncoding R...
ORGANISM(S): Homo sapiens (Human) 
2020-10-14 | PXD015158 | Pride
Cancer Neat1  alternative 3’-end rna processing Integrator Paraspeckles 
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