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ProteomicsBiotin proximity tagging favors unfolded proteins and enables the study of intrinsically disordered regions
Intrinsically Disordered Regions (IDRs) are enriched in disease-linked proteins known to have multiple post-translational modifications, but there is limited in vivo information about how locally unfolded protein regions contribute to biological functions. We reasoned that IDRs should be more access...
ORGANISM(S): Escherichia coli 
2020-01-29 | PXD016422 | Pride
Lc-sps-ms3 E. coli H. sapiens Idr Biotin 
UnknownProbing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging.
Not available
S-EPMC4508323 | biostudies-literature
ProteomicsOff the shelf proximity biotinylation using ProtA-TurboID
Proximity biotinylation is a commonly used method to identify the in vivo proximal proteome for proteins of interest. This technology typically relies on fusing a bait protein to a biotin ligase using overexpression or CRISPR-based tagging, thus prohibiting such assays in (primary) cell types that a...
ORGANISM(S): Homo sapiens (Human) 
2022-10-17 | PXD030963 | Pride
Cell lines Centromeres Biotinylation Histone ptms Protein-protein interactions 
ProteomicsXAB2 involved in RNA processing.
RNA splicing and the DNA damage response are intriguingly linked in mammals but the underlying mechanisms remain poorly understood. Using an in vivo biotinylation tagging approach in mice we show that XAB2, the human homologue of the yeast pre-mRNA-splicing factor SYF1 has a functional role in Nucle...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-08 | PXD014084 | Pride
Snrnas Xab2 Rna processing In vivo biotinylation Interaction analysis Pre-mrnas Nucleotide excision repair 
UnknownUse of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform.
Not available
S-EPMC2544619 | biostudies-literature
UnknownEfficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice.
Not available
S-EPMC164612 | biostudies-literature
TranscriptomicsA native chromatin purification system for epigenomic profiling in C. elegans
We describe an in vivo chromatin purification system for genome-wide epigenetic profiling in C. elegans. In this system, we coexpressed the E. coli biotin ligase enzyme (BirA), together with the C. elegans H3.3 gene fused to BioTag, a 23-amino acid peptide serving as a biotinylation substrate for Bi...
ORGANISM(S): Caenorhabditis elegans 
2010-05-19 | E-GEOD-18898 | biostudies-arrayexpress
UnknownBiotin proximity tagging favours unfolded proteins and enables the study of intrinsically disordered regions.
Not available
S-EPMC6976632 | biostudies-literature
ProteomicsERCC1-XPF Cooperates with CTCF and the Cohesin to Facilitate the Developmental Silencing of Imprinted Genes
Inborn defects in DNA repairare associated with complex developmental disorders whose causal mechanisms are poorly understood. Using an in vivo biotinylation tagging approach in mice, we show that the nucleotide excision repair (NER) structure-specific endonuclease ERCC1-XPF complex interacts with t...
ORGANISM(S): Mus musculus (Mouse) 
2017-04-04 | PXD005897 | Pride
Levers Ap-ms In vivo biotinylation tagging Mouse Ner Label-free Ercc1-xpf 
Unknownβ-Actin mRNA interactome mapping by proximity biotinylation.
Not available
S-EPMC6600913 | biostudies-literature
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