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ProteomicsSugarQuant: a streamlined pipeline for multiplexed quantitative site-specific N-glycoproteomics
Site-specific regulation of protein N-glycosylation is essential in human cells. However, accurate quantification of glycosylation sites and their individual glycan moieties in a cell-wide manner is still technically challenging. Here, we introduce SugarQuant, an integrated mass spectrometry-based p...
ORGANISM(S): Homo sapiens (Human) 
2020-08-24 | PXD018349 | Pride
Glycosylation 
ProteomicsInsulin Resistance in a Type 1 Diabetes Rat Model
A automated 32-plex cPILOT chemical labeling strategy was utilized to identify proteome changes in type 1 diabetes in rats. We utilized an Orbitrap Fusion Lumos mass spectrometer to analyze 8 fractions in technical triplicates where proteins were quantified using Tandem Mass Tag pro reagents (16-ple...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-05-26 | PXD033761 | Pride
Multiplexing Type 1 diabetes Insulin resistance Cpilot 
ProteomicsSelective degradation of platelet BTK by the PROTAC NX-5948
This study presents a tandem mass tag (TMT)–based quantitative proteomic analysis of human platelets treated with the BTK degraders NX-2127 or NX-5948, or vehicle control (DMSO). Washed platelet lysates from three independent biological replicates per condition were digested with trypsin, labelled w...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD072863 | Pride
Human Nx-2127 Quantitative proteomics Nx-5948 Sps-ms3 Tmt Btk Protac Thrombosis Platelets 
ProteomicsInhibition of growth factor signaling prevents SARS-CoV-2 replication
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo sapiens (Human) 
2020-05-15 | PXD018357 | Pride
Infection Sars-cov-2 Phospho Tmt Covid-19 
ProteomicsQuantitative proteomics reveals remodeling of protein repertoire across life phases of Daphnia pulex
Microcrustacean Daphnia is becoming a model organism of choice for ecological and developmental genomics. Yet, Daphnia proteomics resources have so far been rudimental. This study presents LC-MS2/SPS-MS3 proteomes of adult females, juveniles, asexually produces embryos and sexually produced diapausi...
ORGANISM(S): Daphnia Pulex (ncbitaxon:6669) 
2019-04-22 | MSV000083711 | MassIVE
ProteomicsComparison of DIA and TMT based protein quantification in complex background
Label free quantification (LFQ) and isobaric labelling quantification (ILQ) are among the most popular protein quantification workflows in discovery proteomics. Here, we compared the TMT 10-plex workflow to label free single shot data-independent acquisition (DIA) method on a controlled sample set. ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2020-01-29 | MSV000084859 | MassIVE
ProteomicsPXD018357-2 Inhibition of growth factor signaling prevents SARS-CoV-2 replication [Reanalysis: PXD018357]
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Cell Culture 
2023-03-25 | RPXD034696 | JPOST Repository
ProteomicsPXD018357-1 Inhibition of growth factor signaling prevents SARS-CoV-2 replication [Reanalysis: PXD018357]
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Cell Culture 
2023-03-25 | RPXD034695 | JPOST Repository
ProteomicsInhibition of growth factor signaling prevents SARS-CoV-2 replication
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-05-28 | MSV000085494 | MassIVE
ProteomicsRegulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway C4PR_LIV
The ATR-CHK1 signalling pathway responds to single strand DNA breaks and is required for cancer cells to survive the high levels of DNA replication stress and genomic instability resulting from the activity of oncogenes such as MYC. For this reason, inhibitors of CHK1 have great potential as anti-c...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-19 | PXD026203 | Pride
Sps-ms3 Mouse Tmt Phosphorylation Lc-ms/ms 
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