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ProteomicsCharacterization of HAP-1 METTL9 KO cells
LAbel free quantitation of HAP1 WT and METTL9 KO cells. METTL9 is a recently established human histidine specific methyltransferase.
ORGANISM(S): Homo sapiens (Human) 
2021-04-28 | PXD023075 | Pride
Human Cell Lc-ms/ms 
ProteomicsCharacterization of METTL9-mediated methylation of DNAJB12
The analysis is part of a study aimed at characterizing a new human histidine-specific methyltransferase denoted METTL9. The putative METTL9 substrate DNAJB12 was immunoprecipitated from HEK293 cells and its PTM status was assessed by LC-MSMS.
ORGANISM(S): Homo sapiens (Human) 
2020-11-19 | PXD020010 | Pride
Lc-msms Hek293 Methylation 
ProteomicsProteomics exploration of the human RPL3 interactome
Exploring the interactome of human RPL3 by MS-based proteomics.
ORGANISM(S): Homo sapiens (Human) 
2021-09-08 | PXD016055 | Pride
Gfp-pulldown Affinity proteomics Interactomics 
ProteomicseEF1A peptide pull-down LC-MS/MS
Peptide pull-down using synthetic peptides corresponding to unmodified and methylated human eEF1A.
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD008115 | Pride
Methylation Ptm Lc-ms/ms 
ProteomicsSILAC proteome of MTase deficient cells
SILAC proteome of MTase deficient HAP-1 cells and corresponding WT.
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD008131 | Pride
Knockout Methyltransferase Methylation Ptm Silac Lc-ms/ms 
ProteomicsMETTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus, part 2
The experiment aimed at characterizing the methylation status of eEF1A in cells (HeLa) stressed using a panel of drugs including anisomycin, cycloheximide, 4NQO and adenosine dialdehyde (AdOx).
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD009914 | Pride
Methylation Ptm Eef1a 
ProteomicsMETTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus
The experiment aimed at identifying lysine methylation dependent interactions for eEF1A. FLAG-tagged wild type eEF1A and a methylation deficient mutant, carrying lysine-to-arginine mutations of the well-established methylation sites (Lys36, Lys55, Lys79, Lys165 and Lys318) were overexpressed in HEK...
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD009895 | Pride
Methylation Eef1a Interactome 
ProteomicsExploring the interactome of human METTL9
Exploring the interactome of the human putative methyltransferase enzyme METTL9.
ORGANISM(S): Homo sapiens (Human) 
2020-11-19 | PXD016408 | Pride
Mettl9 Interactome 
ProteomicsProSeAM SILAC MS screening for METTL9 substrates
Click chemistry based substrates screening for METTL9 in Mettl9 KO MEF cells.
ORGANISM(S): Mus Musculus (mouse) 
2020-11-19 | PXD016823 | JPOST Repository
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