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ProteomicsDe novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography
Understanding molecular mechanisms of cellular pathways regularly requires knowledge of the identities of participating proteins, their cellular localization and their 3D structures. Contemporary workflows typically require multiple techniques to identify target proteins, track their localization us...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-21 | PXD036885 | Pride
Global protein abundance In situ cryoet Mouse sperm axoneme Sperm flagella Visual proteomics 
ProteomicsStructure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13
Mycobacterium polyketide synthase 13 (Pks13) is a protein complex comprised of a closely symmetric parallel dimer of chains, each encoding several enzymatic and transport functions. This module carries out the condensation of two different very long chain fatty acids to produce mycolic acids that ar...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-13 | PXD033471 | Pride
Xl-ms Dsso Polyketide synthase pks13 Mycobacterium 
ProteomicsNefCD4AP2 Complex Crosslinking with DSSO for Integrative Structure Determination
Objective: HIV-1 Nef suppresses immune surveillance mechanisms to promote viral pathogenesis. Cellular receptor CD4 is essential for HIV entry into host cells, though becomes problematic at later stages in the virus replication cycle. CD4 at the plasma membrane is targeted by Nef for endocytosis an...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 1 
2021-09-09 | PXD019338 | Pride
Xlms Dsso Cd4 Ap2 Hiv-1 nef 
ProteomicsA3G-Vif-CBFB-CRL5 Cross-Linking with DSSO
Here we use an optimization cross-linking mass spectrometry (XL-MS) pipeline for the structural characterization of a dynamic HIV-host protein complex. Using DSSO-based XL-MS analysis, residue-protein proximity restraints based on functional genetics, and integrative modeling, we define the structu...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 
2021-11-03 | PXD025391 | Pride
Xl-ms Dsso Apobec3g Hiv vif Integrative modeling 
ProteomicsSystematic Discovery of Pro- and Anti-HIV Host Factors in Primary Human CD4+ T Cells
Despite extensive efforts, significant gaps remain in our understanding of which host factors promote or restrict human immunodeficiency virus (HIV) infection in human CD4+ T cells. Here, we employed orthogonal genome-wide CRISPR activation (CRISPRa) and CRISPR knockout (CRISPRn) screens in primary ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD071892 | Pride
Host factor Ip-ms Ppi Pi16 Hiv Cd4+ t cells 
ProteomicsA SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Purposing
An outbreak of the novel coronavirus SARS-CoV-2, the causative agent of COVID-19 respiratory disease, has infected over 170,000 people since the end of 2019, killed over 7,400, and caused worldwide social and economic disruption. SARS-CoV-2 infection has a mortality rate of 3.4% among confirmed case...
ORGANISM(S): Severe Acute Respiratory Syndrome Coronavirus 2 (ncbitaxon:2697049) Homo Sapiens (ncbitaxon:9606) 
2020-03-24 | MSV000085144 | MassIVE
ProteomicsThe Global Phosphorylation Landscape of SARS-CoV-2 Infection
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 2019 (COVID-19) pandemic, has infected over 3 million people worldwide and caused over 200,000 deaths since it emerged in late 2019. There is an urgent need to develop therapies that can limi...
ORGANISM(S): Chlorocebus Aethiops (ncbitaxon:9534) 
2020-07-15 | MSV000085759 | MassIVE
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