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ProteomicsDe novo protein identification in mammalian sperm using high-resolution in situ cryo-electron tomography
Understanding molecular mechanisms of cellular pathways regularly requires knowledge of the identities of participating proteins, their cellular localization and their 3D structures. Contemporary workflows typically require multiple techniques to identify target proteins, track their localization us...
ORGANISM(S): Mus musculus (Mouse) 
2024-05-21 | PXD036885 | Pride
Global protein abundance In situ cryoet Mouse sperm axoneme Sperm flagella Visual proteomics 
ProteomicsProtein interaction map of APOBEC3 enzyme family
Human APOBEC3 enzymes are a family of single-stranded (ss)DNA and RNA cytidine deaminases that act as part of the intrinsic immunity against viruses and retroelements. APOBEC3s deaminate cytosine to form uracil which can functionally inactivate or cause degradation of viral or retroelement genomes. ...
ORGANISM(S): Homo sapiens (Human) 
2024-04-16 | PXD044275 | Pride
Apobec3 Ppi Interaction network Ap-ms 
ProteomicsStructure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13
Mycobacterium polyketide synthase 13 (Pks13) is a protein complex comprised of a closely symmetric parallel dimer of chains, each encoding several enzymatic and transport functions. This module carries out the condensation of two different very long chain fatty acids to produce mycolic acids that ar...
ORGANISM(S): Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155) 
2023-06-13 | PXD033471 | Pride
Xl-ms Dsso Polyketide synthase pks13 Mycobacterium 
ProteomicsSecreted ORF8 reprograms macrophages to enhance SARS-CoV-2 infection of lung epithelial cells
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily targets the respiratory epithelium, yet severe disease features diffuse lung injury and hyperinflammatory syndromes driven by dysregulated immune activation. Emerging evidence indicates that resident and infiltrating immune cells...
ORGANISM(S): Homo sapiens (Human) 
2026-08-07 | PXD069220 | Pride
Sars-cov-2  orf8  macrophage  ace2  pyroptosis  il-1β  brodalumab 
ProteomicsGlobal Protein Abundance and Phosphorylation Analyses of Influenza A Virus (IAV)-Infected Human Cells
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD035900 | Pride
Human H5n1 H1n1 Protein abundance Phosphorylation Nhbe H3n2 Thp-1 Infection Influenza a virus 
ProteomicsStrain- and cell type-specific influenza A virus (IAV)-host protein-protein interaction (PPI) network identified by affinity purification-mass spectrometry (AP-MS)
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry and global phosphoproteomic and...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD036077 | Pride
Virus-host H5n1 Human Ppi H1n1 A549 Nhbe H3n2 Thp-1 Ap-ms Influenza a virus 
ProteomicsNefCD4AP2 Complex Crosslinking with DSSO for Integrative Structure Determination
Objective: HIV-1 Nef suppresses immune surveillance mechanisms to promote viral pathogenesis. Cellular receptor CD4 is essential for HIV entry into host cells, though becomes problematic at later stages in the virus replication cycle. CD4 at the plasma membrane is targeted by Nef for endocytosis an...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 1 
2021-09-09 | PXD019338 | Pride
Xlms Dsso Cd4 Ap2 Hiv-1 nef 
ProteomicsA3G-Vif-CBFB-CRL5 Cross-Linking with DSSO
Here we use an optimization cross-linking mass spectrometry (XL-MS) pipeline for the structural characterization of a dynamic HIV-host protein complex. Using DSSO-based XL-MS analysis, residue-protein proximity restraints based on functional genetics, and integrative modeling, we define the structu...
ORGANISM(S): Homo sapiens (Human) Human immunodeficiency virus 
2021-11-03 | PXD025391 | Pride
Xl-ms Dsso Apobec3g Hiv vif Integrative modeling 
ProteomicsEndogenous pull-downs of human proteins ATP6V1A and AHNAK to validate interactions with influenza A virus proteins M2 and NEP by targeted mass spectrometry in virus-infected cells
Influenza A Virus (IAV) is a recurring respiratory virus with antiviral therapies of limited use. Understanding host proteins essential for IAV infection can identify targets for alternative host-directed therapies (HDTs). Using affinity purification-mass spectrometry (AP-MS) and global phosphoprote...
ORGANISM(S): Homo sapiens (Human) 
2023-07-25 | PXD041663 | Pride
Virus-host Protein-protein interaction H5n1 Prm Human A549 Nhbe Influenza a virus 
ProteomicsSystematic Discovery of Pro- and Anti-HIV Host Factors in Primary Human CD4+ T Cells
Despite extensive efforts, significant gaps remain in our understanding of which host factors promote or restrict human immunodeficiency virus (HIV) infection in human CD4+ T cells. Here, we employed orthogonal genome-wide CRISPR activation (CRISPRa) and CRISPR knockout (CRISPRn) screens in primary ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-27 | PXD071892 | Pride
Host factor Ip-ms Ppi Pi16 Hiv Cd4+ t cells 
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