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ProteomicsTranslation proteomics of OSCC CD44/CD36 sorted subpopulations
Metabolic flexibility is a common hallmark of aggressive and metastatic cancers. Here, we reveal that dynamic changes in mitochondrial translation rates drive the switch between glycolytic and mitochondrial energy production required for metastasis. We find that loss of 5-methylcytosine (m5C) in mit...
ORGANISM(S): Homo sapiens (Human) 
2022-06-23 | PXD021835 | Pride
Oscc Proteomics Meprod Translation 
ProteomicsInhibition of growth factor signaling prevents SARS-CoV-2 replication
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo sapiens (Human) 
2020-05-15 | PXD018357 | Pride
Infection Sars-cov-2 Phospho Tmt Covid-19 
ProteomicsUsing instrument logic to improve identifications in low stoichiometry SILAC/TMT experiments
Pulsed SILAC approaches allow measurement of protein dynamics, including protein translation and degradation. However, its use in quantifying acute changes has been limited due the low labeled peptide stoichiometry. Here, we describe the use of instrument logic to select peaks of interest via target...
ORGANISM(S): Homo sapiens (Human) 
2020-05-29 | PXD018445 | Pride
Silac Protein dynamics Tmt 
ProteomicsFunctional translatome proteomics reveal converging regulation by mTORC1 and eIF2α
Regulation of the translatome is essential during stress. However, the precise sets of translational targets regulated by the key translational stress responses –integrated stress response (ISR) and mTORC1 – remain elusive. We developed multiplexed enhanced protein dynamics (mePROD) proteomics, comb...
ORGANISM(S): Homo sapiens (Human) 
2019-12-06 | PXD014377 | Pride
Human Tmt Translation Pulsed silac 
ProteomicsFunctional Translatome Proteomics Reveal Converging and Dose-Dependent Regulation by mTORC1 and eIF2α
Regulation of the translatome is essential during stress. However, the precise sets of translational targets regulated by the key translational stress responses –integrated stress response (ISR) and mTORC1 – remain elusive. We developed multiplexed enhanced protein dynamics (mePROD) proteomics, comb...
ORGANISM(S): Homo sapiens (Human) 
2019-12-09 | PXD015438 | Pride
Proteomics Tmt Psilac Translation 
ProteomicsProteome and Translatome of SARS-CoV-2 infected cells
Cells were infected with SARS-CoV-2 and profiled for translatome and proteome after 2,6,10 and 24 hours
ORGANISM(S): Homo sapiens (Human) 
2020-07-20 | PXD017710 | Pride
Sars-cov-2 Proteome Translatome 
ProteomicsExosome proteomics under hypoxia
TMT multiplexed exosome samples, SEC purified from cells cultured under Normoxia and Hypoxia
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD024044 | Pride
Exosomes Hypoxia 
ProteomicsProteomics of WT or G2A deficient macrophages with 9-HODE
Macrophages from WT or G2A deficient mice were either control or 9-HODE treated and TMT10 multiplexed proteomics performed
ORGANISM(S): Mus musculus (Mouse) 
2020-07-29 | PXD019836 | Pride
Macrophages Mouse Tmt Proteomics 
ProteomicsTargeted multiplexed proteomics of SARS-CoV-2 peptides in ALI cells
Human air-liquid-interface cultured cells (ALI) were infected with SARS-CoV-2 in presence or absence of the drug aprotinin. Targeted proteomics were performed on previously identified peptides modified with TMTpro.
ORGANISM(S): Homo sapiens (Human) 
2020-11-05 | PXD019950 | Pride
Targeted Sars-cov-2 Tmt 
ProteomicsInhibition of growth factor signaling prevents SARS-CoV-2 replication
Caco-2 cells were mock-infected or infected with SARS-CoV-2 patient isolates (in biological quintruplicates) for one hour, washed, and incubated for 24 hours before cell harvest. Extracted proteins were digested and split to 1) carry out whole-cell proteomics of a tandem mass tag (TMT) 10-plex sampl...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-05-28 | MSV000085494 | MassIVE
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