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ProteomicsLFQMS of RING2 (RING1B, RNF2) immunoprecipitation from MDA-MB-231 and T47D breast cancer cells
Polycomb repressive complex PRC1 is essential for gene regulation in numerous cell fate decisions. We show that RING1B (RING2, RNF2) and canonical PRC1 (cPRC1) genes are amplified and overexpressed in breast cancer (BC). Moreover, cPRC1 complexes functionally associate with genes regulated by cell t...
ORGANISM(S): Homo sapiens (Human) 
2018-10-19 | PXD009570 | Pride
Lc-msms lfqms ip-ms ap-ms ring2 
ProteomicsMulti-condition affinity capture/MS analysis of LAP-tagged human NCBP1, NCBP2, and NCBP3
A 5′, 7-methylguanosine cap is a quintessential feature of RNA polymerase II-transcribed RNAs, and thus a textbook aspect of co-transcriptional pre-mRNA processing. The cap is bound by the cap-binding complex (CBC), canonically consisting of nuclear cap-binding proteins 1 and 2 (NCBP1/2). Recently, ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-09 | PXD016038 | Pride
Mrnp Cap binding complex Cbc Interactome screen 
ProteomicsColorectal cancer anti-ORF1p LFQ IP-MS
Here, three colorectal tumors were subjected to anti-ORF1p LFQ, IP-MS: (A) Krukenberg Carcinoma, Ovary; (B) Metastatic Rectal Adenocarcinoma, Liver; (C) Adenocarcinoma, Colon. These were accompanied by matched normal IP controls and/or mouse IgG IP controls. The objective is to map LINE-1 RNP intera...
ORGANISM(S): Homo sapiens (Human) 
2019-08-23 | PXD013743 | Pride
Lc-msms Patient tumor Line-1 L1 Retrotransposon Crc 
ProteomicsLINE-1 ORF1p co-immunoprecipitations analyzed by DDA and two DIA methods
Using an anti-ORF1 monoclonal (clone 4H1; Milipore), four co-immunoprecipitations to enrich for LINE-1 ORF1p and its interactors as described in Di Stefano, L.H. et al. (https://doi.org/10.1007/978-1-0716-2883-6_12), from either HEK-293TLD (cells transfected with pMT646- containing a L1 sequence) or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-07-24 | MSV000095427 | MassIVE
ProteomicsReverse Transcriptase Inhibitors regulate LINE-1 expresion, modulate ORF1p protein interactions, and activate autophagy in cancer cells
Long Interspersed Element-1 (LINE-1; L1) is an autonomous retrotransposon whose expression is frequently dysregulated in cancer. L1 encodes two proteins, ORF1p and ORF2p, which are essential for retrotransposition. Reverse transcriptase inhibitors (RTIs), including nucleoside (NRTIs) and non-nucleos...
ORGANISM(S): Homo sapiens (Human) 
2026-08-17 | PXD082586 | Pride
Human  ip-ms  line-1  orf1p 
ProteomicsAggregation under genotoxic conditions in HEK293T and U2OS cells
This dataset consists of 2 independent experiments. The first research aim was to identify the proteins that aggregate in HEK293T cells treated with the chemotherapeutic agent camptothecin (CPT), the second to identify the proteins that aggregate in U2OS cells after a loss of the checkpoint kinase A...
ORGANISM(S): Homo sapiens (Human) 
2022-04-08 | PXD030166 | Pride
Human Insoluble Genotoxic Cpt U2os Camptothecin Aggregation 
ProteomicsDissection of affinity captured LINE-1 macromolecular complexes
Long Interspersed Nuclear Element-1 (LINE-1, L1) is a mobile genetic element active in human genomes. L1-encoded ORF1 and ORF2 proteins bind L1 RNAs, forming ribonucleoproteins (RNPs). These RNPs interact with diverse host proteins, some repressive and others required for the L1 lifecycle. Using dif...
ORGANISM(S): Homo sapiens (Human) 
2018-01-17 | PXD008542 | Pride
Line-1 Protein interactions Affinity proteomics L1 Mass spectrometry Biochemistry Retrotransposon Ribonucleoprotein Rnp Interactome 
ProteomicsHRMS1 DIA and DDA of Lyso-IP inputs from mouse gut epithelium and stem cells
These mouse gut lysates contained three sets, with Set A including 21 DDA experiments and 21 DIA experiments, Set C including 24 DDA and 24 DIA experiments, and Set G including 27 DDA RAWs and 26 DIA RAWs (one of the Set G DDA experiments was run twice). Experiment Set A was intended to compare lys...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-07-26 | MSV000095444 | MassIVE
ProteomicsLINE-1 ORF2p Structure. BS3 crosslinks
ORF2p was cross-linked using BS3 in order to gather experimental data that could help elucidate its structure.
ORGANISM(S): Homo sapiens (Human) 
2023-12-12 | PXD038615 | Pride
Orf2 orf2p line-1 bs3 structural proteomics 
ProteomicsTargeted mass spectrometry of endogenous LINE-1 proteins and ORF2p interactomics
Expression and activity of LINE-1 (L1) retrotransposons is known to occur in numerous cell-types and is implicated in pathobiological contexts such as aging-related inflammation, autoimmunity, and in cancers. L1s encode two proteins that are translated from bicistronic transcripts. The translation p...
ORGANISM(S): Homo sapiens (Human) 
2025-02-06 | PXD057269 | Pride
Srm Cancer Targeted proteomics Prm Mass spectrometry Line-1 Retrotransposon 
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