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ProteomicsN-terminome Method Development (Discovery)
Developed a method to enrich N-terminome. Several parameters were change to optimization. This project focused on the discovery of N-terminome.
ORGANISM(S): Homo sapiens (Human) 
2020-05-25 | PXD011141 | Pride
Human N-terminome Yeast 
ProteomicsN-terminome analysis of HeLa cells with UPR stress
N-terminome analysis of HeLa proteome under the stress of unfolded protein response. UPR was derived by combinational treatment of MG132 and thapsigargin. N-terminal enrichment was performed by iNrich method (10.1021/acs.analchem.9b05653). Data was prepared to have duplicates.
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD058868 | Pride
Human N-terminome Hela Upr 
ProteomicsN-terminome Method Development
Developed a method to enrich N-terminome. Several parameters were change to optimization
ORGANISM(S): Homo sapiens (Human) 
2020-05-25 | PXD011117 | Pride
Human N-terminome 
ProteomicsAutomated N-glycopeptide identification in glycoproteomics
Recent advances in software-driven glycopeptide identification in LC-MS/MS-based N-glycoproteomics have facilitated biochemical studies reporting thousands of intact N-glycopeptides, i.e. N-glycan-conjugated peptides, but the automated identification process remains to be scrutinized. Herein, we exp...
ORGANISM(S): Homo sapiens (Human) 
2016-08-17 | PXD004243 | Pride
Basigin Byonic Glycoproteomics Glycopeptide N-glycosylation Lc-ms/ms 
ProteomicsYeast N-terminome SILAC - Formyl-methionine as an N-degron of a eukaryotic N-end rule pathway
Discerning yeast N-termionome with SILAC method. To analyze qualitative and quantitative analysis of N-formyl-methionine when introducing environmental stress.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-12 | PXD010780 | Pride
N-terminome Yeast Silac Formyl 
MetabolomicsNMR based metabolomics study of Y2 receptor activation by neuropeptide Y in the SK-N-BE2 human neuroblastoma cell line
Neuropeptide Y (NPY) and its Y2 receptors (Y2R) play an important role in regulating growth of various tumors and are highly expressed in human neuroblastoma brain tumors. Cell lines derived from neuroblastoma brain tumors provide a good model to study the consequences of NPY activation of Y2R in tu...
2014-10-01 | MTBLS104 | MetaboLights
ProteomicsSW480 N-terminal peptide analysis
N-terminal peptide analysis of SW480 cell line for Nt-formylation.
ORGANISM(S): Homo sapiens (Human) 
2023-07-20 | PXD039053 | Pride
Sw480 N-terminome Human Inrich 
ProteomicsN-terminome profiling in HEK293T
Various forms of the protein, called proteoform, are generated by co- or post-translational modification, alternative splicing and alternative translation initiation site, resulting in interactions between ribosomes, mRNAs, tRNAs, and various enzymes. Here, we introduce a N-terminal peptide enrichme...
ORGANISM(S): Homo sapiens (Human) 
2017-08-17 | PXD005583 | Pride
Protein stability Alternative translational initiation site Acetylation N-terminome Positional proteomics Proteoform N-termini Fasp N-end rule Translational initiation site 
ProteomicsNaa10 mutant mouse N-terminome LC-MS
This project have LC-MSMS analysis results of label free quantitation of N-terminome enrichment of mouse proteome. N-terminal modification of mouse embryonic fibroblasts (MEFs) lacking Naa10 show negligible difference between wild-type and Naa10 mutant.
ORGANISM(S): Mus musculus (Mouse) 
2021-08-18 | PXD026410 | Pride
Lc-msms N-terminome Mef Naa10 Inrich Lfq Mouse 
ProteomicstipNrich, a tip-based N-terminal proteome enrichment method
Protein N-termini provide valuable information such as half-life of protein, but one of hurdles to study N-terminome is that it requires large amount of sample, but this requirement might be problematic for analyzing the limited samples such as low number of cells of clinical biopsies. In this regar...
ORGANISM(S): Bos taurus (Bovine) Mus musculus (Mouse) Escherichia coli Bacillus subtilis 
2022-12-05 | PXD027961 | Pride
Human plasma Recombinant single proteins Protein mixture Lc-ms/ms 
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