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ProteomicsAnalysis of PKD1 autophosphorylation in vitro and confirmation of stoichiometric S742 phosphorylation of a recombinant PRKD1 kinase domain
Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo autophosphorylation on serine 742 and potentially also on serine 738 in the activation loop of its kinase domain. We used recombinant PKD1 kinase domain to test whether PKD1 is indeed able to undergo autophosphorylatio...
ORGANISM(S): Homo sapiens (Human) 
2019-08-19 | PXD013216 | Pride
Pkd1 Protein kinase d 
ProteomicsCellular concentrations of Protein Kinase D1 in HEK293T and INS-1 cells
Protein kinase D1 (PRKD1, also referred to as PKD1), has been proposed to undergo concentration dependent dimerization in vitro. To test whether the cellular concentration of PKD1 is in the range of the dissociation constant obtained, the expression level of PKD1 was investigated by PRM. To this end...
ORGANISM(S): Homo sapiens (Human) Rattus norvegicus (Rat) 
2019-08-19 | PXD013232 | Pride
Pkd1 Ins-1 Protein kinase d Hek293t 
ProteomicsCross-link mass spectrometry reveals conformational changes within the N lobe and the activation loop of PDK1 upon dimerization
Cross-linking mass spectrometry analysis of the PDK1 kinase domain dimer that undergoes trans-autophosphorylation.
ORGANISM(S): Homo sapiens (Human) 
2022-05-20 | PXD031827 | Pride
Pip3 Dimerization Phosphorylation Kinase Pdk1 
ProteomicsMapping of phosphorylation sites on Sgk3 by LC-MS/MS
The phosphorylation profile of Sgk3 protein expressed either in Sf9 insect cells or HEK 293T human cells was assessed by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026281 | Pride
Activation loop Phosphorylation Sgk3 Turn motif Hydrophobic motif 
ProteomicsMapping and quantification of phosphorylation sites on protein kinase Akt by LC-MS/MS
Mass spectrometry analysis of various Akt species was a cornerstone for elucidating the mechanism of its activation/autoinhibition. By combining intact mass measurements with LC-MS/MS we were able to characterize Akt species comprehensively and relate their biological activity to specific phosphoryl...
ORGANISM(S): Homo sapiens (Human) 
2021-08-13 | PXD022044 | Pride
Growth factor signaling Pip3 Akt1 Lipid second messengers 
ProteomicsInvestigating the monomer-dimer transition of PKD1 kinase domain with EDC crosslinking
The project aim was to generate experimental constraints for the in silico modeling of the dimeric structure of the PKD kinase domain. And identify conformational changes upon dimerization. For this we used the heterobifunctional zero length crosslinker EDC to identify salt bridges within the protei...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD031997 | Pride
Protein kinase d Kinase domain dimer Activation loop ordering 
UnknownLong-term update of US GI intergroup RTOG 98-11 phase III trial for anal carcinoma: survival, relapse, and colostomy failure with concurrent chemoradiation involving fluorouracil/mitomycin versus fluorouracil/cisplatin.
Not available
S-EPMC3515768 | biostudies-literature
ProteomicsPeptide fingerprinting of purified recombinant human PHLPP2
Peptide fingerprinting to establish whether there is any contaminating phosphatase(s) in the purified protein.
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) 
2025-03-13 | PXD045978 | Pride
Human phlpp2 
ProteomicsPeptide fingerprinting of eluates obtained from phosphatase inhibitor beads (PIBs)
Peptide fingerprinting to verify the efficacy of phosphatase inhibitor beads (PIBs) for capturing Spodoptera frugiperda (Sf9) phosphatases of the PP2A family.
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) 
2025-03-13 | PXD045891 | Pride
Human phlpp2 Phosphatase inhibitor 
ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
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