Sort   by:  
 Page size 
ProteomicsUbiquitome analysis of UHRF1 and UHRF2-deficient mESCs
The E3 ligase UHRF1 is an essential epigenetic cofactor for DNMT1 dependent maintenance DNA methylation, which provides a binding platform for DNMT1 by both cooperative binding of histones and hemi-methylated DNA as well as by ubiquitinating histone H3. Here, we conduct a comprehensive screen to ide...
ORGANISM(S): Mus musculus (Mouse) 
2017-10-24 | PXD006593 | Pride
Ubiquitination Paf Mouse embryonic stem cells Uhrf 
ProteomicsComprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components
The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we develope...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-02-27 | PXD034448 | Pride
Naive Dia Pluripotency Mass spectrometry Stem cells Formative Chromatin proteomics Primed 
ProteomicsChIP-MS of HP1-beta (CBX1) and ubiquitylome of KAP1 (TRIM28) in mESCs
Heterochromatin binding protein HP1β plays an important role in chromatin organization and cell differentiation, however the underlying mechanisms remain unclear. Here, we generated HP1β-/- embryonic stem cells and observed reduced heterochromatin clustering and impaired differentiation. We found th...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-24 | PXD025053 | Pride
Phase separation Pluripotency exit Chromocenter Heterochromatin Chromatin structure 
ProteomicsChIP-MS of KAT7, H3K9me3 and H3K4me3 in naive, formative and primed mouse PSCs
The establishment of cellular identity is driven by transcriptional and epigenetic regulation exerted by the components of the chromatin proteome - the chromatome. However, chromatome composition and its dynamics in functional phases of pluripotency have not been comprehensively analyzed thus limiti...
ORGANISM(S): Mus musculus (Mouse) 
2023-02-27 | PXD039556 | Pride
Epigenetics Chip-ms Pluripotency Interactome 
ProteomicsIdentification of ubiquitination targets of UHRF1
The reversible attachment of ubiquitin governs the interaction, activity and degradation of proteins whereby the type and target of this conjugation determine the biological response. The investigation of this complex and multi-faceted protein ubiquitination mostly relies on painstaking biochemical ...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-30 | PXD034335 | Pride
Posttranslational modifications (pmt) Ubiquitination Protein degradation Dna methylation Heterochromatin binding protein (hp1) Uhfr1 
TranscriptomicsIdentification and characterization of two novel primate-specific histone H3 variants H3.X and H3.Y
Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here we report the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their mRNAs are found in certain human cell ...
ORGANISM(S): Homo sapiens 
2010-09-17 | E-GEOD-22415 | biostudies-arrayexpress
TranscriptomicsThe nucleosome remodeler SNF2L modulates WNT signaling for cellular homeostasis
ISWI is an evolutionary conserved ATPase that catalyzes nucleosome remodeling in several different complexes. Two mammalian ISWI orthologs, SNF2H and SNF2L, have specialized functions despite their high similarity. Due to the lack of reagents the functions of SN2L in human cells had not been establi...
ORGANISM(S): Homo sapiens 
2012-05-10 | E-GEOD-30471 | biostudies-arrayexpress
UnknownRad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production.
Not available
S-EPMC4309842 | biostudies-literature
ProteomicsMm_mChmC_DynamicReader
Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives
ORGANISM(S): Mus Musculus 
2013-12-31 | PAe004058 | PeptideAtlas
UnknownCoherent spectroscopy with a single antiproton spin.
Not available
S-EPMC12328203 | biostudies-literature
Sort   by:  
 Page size