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ProteomicsAmino acid analog induces stress response in marine Synechococcus
In this study, we explored the use of BONCAT in Synechococcus sp. – a globally important cyanobacteria. We characterized the growth and microscopically quantified HPG uptake under a range of HPG concentrations in marine Synechococcus sp. Further, we examined changes in protein expression of Synechoc...
ORGANISM(S): Synechococcus sp. CC9311 
2021-04-01 | PXD023371 | Pride
Boncat Phytoplankton Synechococcus Ncaas 
ProteomicsLONP1 and mtHSP70 cooperate to promote mitochondrial protein folding
Because 99% of the mitochondrial proteome is encoded by the nucleus, most mitochondrial precursor polypeptides are imported from the cytosol into the mitochondrion, where they must efficiently fold into their functional state. Mitochondrial precursors are imported as unfolded polypeptides due to th...
ORGANISM(S): Homo sapiens (Human) 
2020-12-07 | PXD021939 | Pride
Aggregates Protein homeostasis Mitochondria Human 
ProteomicsA proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes
Oxysterols (OHCs) are hydroxylated cholesterol metabolites that play ubiquitous roles in health and disease. Due to the non-covalent nature of their interactions and unique partitioning in membranes, the analysis of live-cell, proteome-wide interactions of OHCs remains an unmet challenge. In this Re...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-29 | PXD027787 | Pride
Chemoproteomics probe Cancer Oxysterols Osteogenic molecules Immune response Live-cell analysis 
ProteomicsA Systematic Interrogation of MHC Class I Antigen Presentation Identifies Constitutive and Compensatory Protein Degradation Pathways
Peptides presented by MHC Class I were purified from B lymphoblasts or breast cancer cell lines following protein degradation pathway perturbations. Approximately 1E8 total cells were used for each experiment.
ORGANISM(S): Homo sapiens (Human) 
2023-08-03 | PXD032167 | Pride
Autophagy Mhc class i Neddylation Proteasome Ubiquitin Vcp Immunopeptidome P97 
ProteomicsCSN5i-3 induces degradation of selective CRL substrate receptors and accumulation of CRL substrates
In this study, we used quantitative proteomics mass spectrometry with 16-plex TMT labeling to compare individual protein levels of DMSO and 1 μM CSN5i-3-treated K562 cells for 2, 8, and 24 hours. CSN5i-3 is a selective and potent inhibitor of Cop9 Signalosome (CSN), which regulates the activity of C...
ORGANISM(S): Homo sapiens (Human) 
2022-09-05 | PXD027862 | Pride
Crl Substrate receptor Mass spectrometry Csn5i-3 Cop9 signalosome Quantitative proteomics 
Proteomicsactivin A treated Th17 cells from mouse
Whole cell extracts from mouse Th17 cells treated with activin A were profiled with extensive fractionation and bottom-up proteomics
ORGANISM(S): Mus musculus (Mouse) 
2020-05-18 | PXD017757 | Pride
Th17 cells Mouse Activin a 
ProteomicsThe fatty liver disease-causing protein PNPLA3-I148M alters lipid droplet-Golgi dynamics
Non-alcoholic fatty liver disease (NAFLD), recently renamed metabolic dysfunction-associated steatotic liver disease (MASLD), is a progressive metabolic disorder that begins with aberrant triglyceride accumulation in the liver and can lead to cirrhosis and cancer. A common variant in the gene PNPLA3...
ORGANISM(S): Homo sapiens (Human) 
2024-04-10 | PXD046335 | Pride
Endosome Masld Golgi Liver disease Pnpla3 Lipid droplet Nafld 
ProteomicsQuantitative Measurement of the Requirement of Diverse Protein Degradation Pathways in MHC Class I Peptide Presentation
The adaptive immune system distinguishes self from non-self by surveying peptides generated from degradation of intracellular proteins that are loaded onto MHC Class I molecules for display on the cell surface. While early studies reported that the bulk of cell surface MHC Class I complexes require ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-02-15 | MSV000091285 | MassIVE
TranscriptomicsExpression data from Saccharomyces cerevisiae Met30 temperature-sensitive strain
The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable. We used a yeast-based high-throughput chemical genetic screen to identi...
ORGANISM(S): Saccharomyces cerevisiae 
2011-06-30 | E-GEOD-22270 | biostudies-arrayexpress
TranscriptomicsExpression data from Saccharomyces cerevisiae temperature-sensitive strains specific for SCF core components (Skp1, Cullin (Cdc53), E2 enzyme (Cdc34)) and the F-box protein Cdc4.
The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable. We used a yeast-based high-throughput chemical genetic screen to identi...
ORGANISM(S): Saccharomyces cerevisiae 
2011-06-30 | E-GEOD-22271 | biostudies-arrayexpress
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