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ProteomicsTargeted mass spectrometry of Tau preparations purified from Alzheimer’s Disease brains plus either the deubiquitylase USP21 or its activity-dead mutant C221A.
To characterise the abundance of ubiquitylated Tau peptides in the presence of active or inactive variants (wt 196-565 vs. C221A) of the broad spectrum deubiquitylase USP21. Tau peptides are obtained from Tau fibrils purified from post mortem human brains diagnosed with Alzheimer’s. Their detection ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-18 | PXD046645 | Pride
Alzheimer’s disease (de)ubiquitination/ubiquitylation Neurodegeneration 
ProteomicsIsolation and mass spectrometry analysis of ATG9A vesicles from HAP1 cells.
Autophagy is a conserved cellular degradation pathway involving the formation of double-membrane autophagosomes that engulf cytoplasmic material for delivery to the lysosome. Autophagy-related (ATG) proteins orchestrate the steps of autophagosome formation and maturation, among which ATG9A is unique...
ORGANISM(S): Homo sapiens (Human) 
2026-08-04 | PXD066668 | Pride
Human Hap1 cells Atg9a vesicles 
ProteomicsIdentification of global proteome changes upon rapalog-induced tethering of WIPI2 to the mitochondrial outer membrane in wild-type or NIX/BNIP3 DKO HeLa cells
We aimed to identify proteins that would be turned over upon artificial tethering of WIPI2 to the mitochondrial surface upon rapalog treatment, using the FIS1-FRB plus FKBP-GFP-WIPI2 tethering system. We had previously observed that this induces mitophagy based on flow cytometry analysis of the mt-m...
ORGANISM(S): Homo sapiens (Human) 
2025-01-30 | PXD060363 | Pride
Mitophagy Wipi2 Bnip3 Hypoxia Mitochondria Nix 
ProteomicsIdentification of global proteome changes upon DFP treatment of HeLa cells
We aimed to identify proteins that would be turned over upon DFP treatment, a small molecule that mimicks hypoxia treatment and which induces NIX/BNIP3 mitophagy. We aimed to identify which proteins are degraded upon 24 h DFP treatment and verified whether there was an enrichment of mitochondrial pr...
ORGANISM(S): Homo sapiens (Human) 
2025-01-30 | PXD060356 | Pride
Mitophagy Bnip3 Dfp Hypoxia Mitochondria Nix 
ProteomicsIdentification of NIX-GST and BNIP3-GST interactors
We aimed to identify proteins that interact with the mitophagy receptors NIX or BNIP3. To this end, we incubated recombinantly purified NIX-GST or BNIP3-GST with HeLa cell lysate to identify proteins that would bind to NIX and BNIP3.
ORGANISM(S): Homo sapiens (Human) 
2025-01-30 | PXD060351 | Pride
Mitophagy Wipi2 Bnip3 Pull-down Nix 
ProteomicsMass spectrometry analysis of in vitro Tau ubiquitination by the UBA1/Hsp70/CHIP machinery
We aimed at characterizing which lysines on Tau were ubiquitinated by the UBA1/Hsp70/CHIP machinery. We also determined the ubiquitin linkages of the assembled Tau chains.
ORGANISM(S): Homo sapiens (Human) 
2024-06-18 | PXD034527 | Pride
Ubiquitination Protein degradation Alzheimer’s disease Tau 
ProteomicsIsolation and mass spectrometry analysis of Atg9 vesicles from Saccharomyces cerevisiae
Autophagy is a eukaryotic bulk degradation pathway that allows cells to degrade potentially harmful cytosolic components or to provide necessary nutrients during starvation. This cargo degradation is achieved by its sequestration within double membrane vesicles termed autophagosomes, which fuse with...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-03 | PXD020359 | Pride
Autophagy Atg9 Vesicles Yeast 
ProteomicsIdentification of phosphorylation sites on NAP1/AZI2
We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) res...
ORGANISM(S): Homo sapiens (Human) 
2024-04-12 | PXD049184 | Pride
Azi2 Nap1 Mitophagy Tbk1 
UnknownTECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposure
Not available
S-SCDT-10_15252-EMBJ_2022113012 | biostudies-other
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