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ProteomicsSynaptosome proteomics of tetanus neurotoxin-injected mice
Network hyperexcitability is manifested as frequent ictal discharges, often caused by unbalanced neurotransmission. We assessed synaptic changes in the hyperexcitable visual cortex of tetanus neurotoxin-injected mice (TeNT). A proteomics analysis of synaptic content revealed Carboxypeptidase E (CPE)...
ORGANISM(S): Mus musculus (Mouse) 
2021-09-09 | PXD022270 | Pride
Tetanus neurotoxin Tmt Epilepsy Synaptosome 
ProteomicsNeuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.Neuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.Neuron-Derived Exosomes as a tool for the study of Parkinson disease: a rapid workflow for morphology, miRNA and proteomic characterization.
Neuron derived exosomes have been enriched from plasma using L1CAM antigen. Vesicles were enriched from plasma of Parkinson Disease patients and controls. The NDE morphology was observed by transmission electron microscope (TEM). miRNA-155 content was studied both in PD-NDE and control-NDE. LC-MS/MS...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD020794 | Pride
Proteomics Parkinson disease Neuron derived exosomes Plasma L1cam 
ProteomicsQuantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease
In-depth characterization of the proteome of the Twitcher mouse, a mouse model for Krabbe disease, to investigate the proteome changes in the Central and Peripheral Nervous System.We applied a TMT-based workflow to compare the proteomes of the corpus callosum, motor cortex and sciatic nerves of litt...
ORGANISM(S): Mus musculus (Mouse) 
2019-04-04 | PXD010594 | Pride
Proteomics Krabbe Tmt Corpus callosum Twitcher mouse Motor cortex Sciatic nerves 
ProteomicsUltraviolet Photodissociation of ESI and MALDI generated protein ions on a Q-Exactive mass spectrometer
UVPD was implemented on an Orbitrap Q-Exactive plus equipped with a ESI/EP-MALDI. UVPD of MALDI generated ions was benchmarked against MALDI-ISD, MALDI-HCD and ESI-UVPD. MALDI UVPD outperformed MALDI HCD and ISD efficiently sequencing small proteins ions.
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2019-01-24 | PXD011526 | Pride
Ms/ms Uvpd Q-exactive Maldi 
ProteomicsNovel PPT+SEC Workflow for High-Sensitivity Extracellular Vesicle Proteomics from Cell Media
Size exclusion chromatography (SEC) is a well-established method for the isolation of extracellular vesicles (EVs), but the large elution volumes necessitate a concentration step prior to proteomics analysis. This concentration step can lead to significant EV loss. Here we report an EV proteomics ap...
ORGANISM(S): Homo sapiens (Human) 
2025-05-03 | PXD060798 | Pride
Micro proteomics Extracellular vesicles Pancreatic cancer 
ProteomicsProteomic analysis of serum small extracellular vesicles of individual mice for longitudinal investigations
Characterization of small extracellular vesicles (sEV) from individual mice for the longitudinal investigation of proteome changes in a mouse model of Glioblastoma Multiforme (GBM). We first compared precipitation and size exclusion chromatography (SEC) for sEV purification from 100ul of mouse serum...
ORGANISM(S): Mus musculus (Mouse) 
2020-11-30 | PXD016473 | Pride
Glioblastoma Proteomics Size exclusion chromatography Small extracellular vesicles Serum Mouse Biomarker discovery 
ProteomicsInvestigation of region specific lipid alterations in symptomatic atherosclerotic plaques using mass spectrometry imaging
Atherosclerotic plaques are composed by different cell populations that interact one with each other in a complex and heterogeneous environment. Here we report a MALDI mass spectrometry imaging investigation of the lipid profile of specific plaque regions, to discriminate between symptomatic and asy...
ORGANISM(S): Homo sapiens (Human) 
2021-09-10 | PXD023663 | Pride
Plaque vulnerability Macrophages Maldi msi Lipids Atherosclerosis Vsmcs 
ProteomicsHISTOLOGICAL AND PROTEOMIC CHARACTERIZATION OF MUSCULOSKELETAL AMYLOIDOMAS
We report the histological and immunohistochemical features of four cases of musculoskeletal amyloidoma in association with combined laser capture microdissection (LCM) of Congo Red (CR)-positive regions with a recent microproteomics workflow that improves the sensitivity of the analysis in order to...
ORGANISM(S): Homo sapiens (Human) 
2025-07-14 | PXD031789 | Pride
Proteomics Amyloidoma Lc-ms/ms 
UnknownLow-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics.
Not available
S-EPMC10618491 | biostudies-literature
ProteomicsCandidate early biomarkers of Glioblastoma Multiforme revealed by longitudinal bottom-up proteomics study on multiple biofluids in a murine model
Glioblastoma Multiforme (GBM) is an aggressive form of brain tumor, associated with poor prognosis and low survival rates, and early diagnosis is impaired by the lack of presymptomatic biomarkers. Here we used a syngenic GBM mouse model to investigate longitudinal changes in the proteome profile of ...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD020285 | Pride
Proteomics Extracellular vesicles Glioblastoma Serum Csf. Biomarkers Longitudinal 
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