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ProteomicsDiscovering the N-terminal Methylome by the Repurposing of Proteomic Datasets
Protein ⍺-N-methylation is a cryptic and relatively unexplored post-translational modification involving the covalent addition of methyl groups to the free a-amino group at protein N-termini. To systematically explore the extent of ⍺-N-terminal methylation in yeast and humans, we utilized a repurpos...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-05-26 | PXD022833 | Pride
Proteomics Post-translational modification Repurposing ⍺-n-terminal methylation 
ProteomicsInvestigating N-terminal shedding of GPR125 in GPR125 overexpressing HEK293T cells using N- and C-terminal enrichment and different cleavage strategies
The study was conducted to elucidate potential cleavage sites of GPR125 which are known to undergo N-terminal shedding through autoproteolysis in a region different to other GRPs. To do these different enzymatic strategies as well as enrichment of both N-terminal and C-terminal peptides, was conduct...
ORGANISM(S): Homo sapiens (Human) 
2026-08-31 | PXD083139 | Pride
Gpr125 Scx C-terminal Lysate Hek293t Enrichment Lc-msms Adra3 N-terminal Medium 
ProteomicsDeveloping a Novel and Integrative Method to Systematically Investigate the Stability of N-Terminal Proteoforms
Protein N-terminal proteoforms, which include both N-terminal modifications and sequence variation arising from processes such as proteolytic cleavage, are common in cells and have been reported to significantly impact protein stability. Systematically examining the correlation between these N-termi...
ORGANISM(S): Homo sapiens (Human) 
2025-08-21 | PXD057760 | Pride
Thermal proteome profiling N-terminal acetylation N-terminome Thermal stability difference Terminal amine isotopic labeling of substrates Tails Tpp N-terminal proteoform 
ProteomicsDifferential alkylation of PHLPP2
To determine the accessibility of cysteines in purified PHLPP2, we assessed their reactivity with iodoacetamide under native conditions. After denaturing with urea and reducing potential disulfide bridges with TCEP, the remaining free cysteines were modified with methyl methanethiosulfonate. This 2-...
ORGANISM(S): Homo sapiens (Human) 
2025-03-13 | PXD052551 | Pride
Differential alkylation Free thiols 
ProteomicsN-terminal acetylation of yeast NAT-A mutants
Protein-protein interactions are fundamental to all cellular activities, with the ribosome increasingly recognized as a central platform steering the dynamics of nascent-chain interactions. In our focus on conserved N-terminal acetyltransferases (NATs), we identified distinct co-translational assemb...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-06-22 | PXD048082 | Pride
N-terminal acetylation Naa15 Naa10 Co-translational assembly Terminomics 
ProteomicsA snapshot of the Physcomitrella N-terminome reveals N-terminal methylation of organellar proteins
Post- or co-translational modifications of protein N-termini influence their half-life and mediate protein sorting to organelles via cleavable N-terminal sequences that are recognized by the respective translocation machinery. In the present study, we provide an overview on the current modification ...
ORGANISM(S): Physcomitrella patens subsp. patens (Moss) 
2025-05-07 | PXD052824 | Pride
Physcomitrium Methylation Moss Mass spectrometry N-terminus 
ProteomicsNHS Carbamate Cross-Linkers Provide Complementary XL-MS Restraints via Preferential N-Terminal Targeting
Cross-linking mass spectrometry (XL-MS) has become a valuable tool for investigating the structural morphology and plasticity of proteins. Traditional cross-linkers contain two N-hydroxy succinimide (NHS) esters that mainly react with lysine residues. In this work, we optimized the in-solution react...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-03-30 | PXD069252 | Pride
Crosslinking ms Cross-linking ms Dsso carbamate Nhs carbamate cross-linkers N-terminal reactivity Xl-ms Cross-linking mass spectrometry Ms-cleavable cross-linkers 
ProteomicsFast Profiling of Protease Specificity reveals similar substrate specificities for cathepsins K, L and S
N-terminal COFRADIC analysis of cathespine K, L and S to obtain the substrate specificity profile of these cysteine cathepsins.
ORGANISM(S): Homo sapiens (Human) 
2015-02-10 | PXD001536 | Pride
Cathespins N-terminal cofradic 
UnknownThe tobacco smoke component, acrolein, suppresses innate macrophage responses by direct alkylation of c-Jun N-terminal kinase.
Not available
S-EPMC3262655 | biostudies-literature
ProteomicsThe impact of commonly used alkylating agents on artefactual peptide modification
Iodoacetamide is by far the most commonly used agent for alkylation of cysteine during sample preparation for proteomics. An alternative, 2-chloroacetamide, has been recently suggested to reduce the alkylation of residues other than cysteine, such as the N-terminus, Asp, Glu, Lys, Ser, Thr and Tyr....
ORGANISM(S): Rattus norvegicus (Rat) 
2017-08-16 | PXD007071 | Pride
Post-translational modification Cysteine alkylation Artefact N-terminal alkylation Alkylation 
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