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ProteomicsThe 15-min Subcellular Proteome
This project integrated data-independent acquisition (DIA) with capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable fast single-cell proteomics. With <15 min of effective separation time in a bottom-up proteomics setting, this technology returned ~1,200 proteins...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2024-09-27 | PXD046467 | Pride
Blastomere Proteomics Stem cell Xenopus laevis Mass spectrometry Data independent acquisition Single cell Capillary electrophoresis 
ProteomicsCapillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics
This project used a custom-built capillary electrophoresis (CE) mass spectrometry (MS) platform to demonstrate the scalable analysis of proteins from single cells of varying sizes and protein content in different vertebrate biological models. Neural tissue fated giant cells were identified in cleava...
ORGANISM(S): Mus musculus (Mouse) Xenopus laevis (African clawed frog) 
2022-03-02 | PXD031955 | Pride
Proteomics Capillary electrophoresis Mass spectrometry Subcellular Scalability 
ProteomicsA Data-Dependent Acquisition Ladder for Ultrasensitive (Neuro)Proteomics
We report here a data acquisition strategy that expands the detectable and quantifiable proteome from trace amounts of protein digests using microanalytical capillary electrophoresis (µCE) electrospray ionization (ESI) high-resolution mass spectrometry (HRMS). Data-dependent acquisition (DDA) was pr...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-18 | PXD027428 | Pride
Neuron Neuroscience Proteomics Mouse. Mass spectrometry Capillary electrophoresis Data dependent acquisition 
ProteomicsElectrophoresis-Correlative Framing of Ion Mobility Deepens Single-Cell Mass Spectrometry Proteomics
This project developed electrophoresis-correlative (Eco) mass spectrometry (MS) to measure the proteome of single cells on a trapped ion mobility mass spectrometer (timsTOF PRO). Eco-MS identified 962 protein groups in a <20 min of electrophoresing a single-HeLa-cell-equivalent (~200 pg) proteome am...
ORGANISM(S): Homo sapiens (Human) Xenopus laevis (African clawed frog) 
2025-05-07 | PXD057685 | Pride
Proteomics Xenopus laevis Mass spectrometry Single cell Capillary electrophoresis 
ProteomicsPatch-Clamp Proteomics of Single Neurons in Tissue Using Electrophysiology and Subcellular Capillary Electrophoresis Mass Spectrometry
In this project, we enabled mass spectrometry based proteomics characterization of single neurons in sections of the mouse brain. We accessed cells in a whole-cell patch clamp configuration and aspirated a portion of the neuronal soma into the patch clamp probe. The collected aspirate was expelled ...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-18 | PXD028040 | Pride
Electrophysiology Mass spectrometry Nervous system Bottom-up proteomics Capillary electrophoresis Whole-cell patch-clamp Mouse 
Proteomics"Time-resolved quantitative proteomic analysis of the developing otic vesicle reveals putative congenital hearing loss candidates and a novel role for Tgfbi
A large number of congenital hearing loss cases have an unknown genetic etiology. So far, transcriptomic approaches have successfully identified many candidate regulators of otic development, little is known about the abundance of their protein products during the development of the inner eat. Herei...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2023-10-24 | PXD041991 | Pride
Otic development Congenital hearing loss Xenopus laevis Temporal Extracellular matrix 
ProteomicsDilute to Enrich for Deep Proteomics: Yolk-Depleted Carrier for a Limited Population of Embryonic (Frog) Cells
This project developed a proteomic methodology that deepens the detectable and quantifiable proteome from limited proteomes where abundant proteins pose interference. As proof-of-principle, this study used Xenopus laevis, where ~90% of the proteome is dominated by abundant yolk proteins in the early...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-05-21 | PXD044787 | Pride
Liquid chromatography Proteomics Xenopus Ultrasensitive Mass spectrometry. Yolk 
ProteomicsProteome-Driven Phenotyping of Identified Single Neurons in Intact Brain Tissue by Capillary Electrophoresis–timsTOF Mass Spectrometry Patch Proteomics
This project developed an ultrahigh-sensitivity proteomics platform in the discovery setting to profile the proteome of cell bodies (somas) between different types of neurons in the mouse central nervous system. The soma contents of mitral cells, serotonergic neurons, dopaminergic neurons, and parva...
ORGANISM(S): Mus musculus (Mouse) 
2026-09-28 | PXD077382 | Pride
Serotonin Dopamine Parvalbumin neuron Whole-cell patch clamp Mass spectrometry Fluorescence label Nervous system Bottom-up proteomics Olfactory bulb Mitral cell Capillary electrophoresis Mouse 
ProteomicsProteome Profiling Among Aging Cell Lines
Characterization of the proteome provides direct information on disease-associated pathways during the onset and progression of Alzheimer’s Disease (AD). In this project, we employed high-resolution mass spectrometry to quantify the proteomes of AD cell cultures using multiplexed quantification. The...
ORGANISM(S): Homo sapiens (Human) 
2026-01-11 | PXD062605 | Pride
Neuroinflammation Proteomics Progeria Mass spectrometry Profiling Aging Biomarkers 
ProteomicsSingle-cell Proteomics of Developing Cells in Live Vertebrate Embryos by Microprobe High-Resolution Mass Spectrometry
In this work, we developed a microprobe capillary electrophoresis high-resolution mass spectrometry approach to identify proteins in single identified cells in live embryos of the South African clawed frog (Xenopus laevis) and also zebrafish. We used a pulled borosilicate capillary to aspirate a cal...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Xenopus laevis (African clawed frog) 
2020-05-26 | PXD006905 | Pride
Blastomere Embryo Proteomics Xenopus Mass spectrometry Label free quantification Lfq Capillary electrophoresis Electrospray ionization Orbitrap Q-exactive plus Single cell analysis 
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