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ProteomicsMycobacterial strain-specific temporal modulations of newly synthesized macrophage secretome
THP-1 macrophages were infected with four strains of Mycobacterium tuberculosis to study the temporal dynamics of newly synthesized proteins in the secretome. Temporal snapshots of secretome reflect the macrophage response to pathogenicity which in combination with intracellular events, completes th...
ORGANISM(S): Homo sapiens (Human) 
2016-08-10 | PXD004281 | Pride
Boncat Mtb infection Hyperplexing Newly synthesized Silac Temporal Itraq Secretome 
ProteomicsAutomated method for enrichment of newly synthesized proteins based on AHA labeling
In this study, a novel high throughput method was successfully developed for the automated enrichment of newly synthesized proteins (NSPs) from complex environments, like cell culture media supplemented with fetal bovine serum (FBS). The proposed automated method allows for the robust and effective ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025255 | Pride
High throughput Newly synthesized proteins Aha Lc-ms Interferon Melanoma Enrichment Proteomics Click chemistry 
ProteomicsSystematic evaluation of protein synthesis inhibition through global quantification of newly synthesized protiens
Manipulating protein synthesis is commonly applied in bio-related research to uncover protein functions and cellular activities. Multiple inhibitors with distinct mechanisms are widely used, but it is extraordinarily challenging to systematically determine and evaluate their inhibition efficiencies,...
ORGANISM(S): Homo sapiens (Human) 
2023-05-24 | PXD035992 | Pride
Bioorhogonal chemistry  protein synthesis inhibtion  protein inhibition efficiency  mass spectromety  multiplexed proteomics  newly synthesized proteins 
GenomicsIdentification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
PRJEB70937 | ENA
ProteomicsNewly synthesized proteome analysis of interferon gamma treated Hela cells
Using a newly-developed workflow for quantitative newly synthesized proteome analysis (QuaNPA), featuring automated sample processing and multiplexed DIA (plexDIA) analysis, changes in the newly synthesized proteome of IFN-gamma treated Hela cells were monitored over time.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD038915 | Pride
Dia Nascent proteome analysis Automated sample processing Interferon gamma Silac 
Proteomicssemi-automated enrichment of AHA-containing newly-synthesized proteins with magnetic alkyne agarose beads
Development of an improved workflow for newly synthesized proteome analysis, based on combined pulsed metabolic labelling with L-azidohomoalanine (AHA) and semi-automated click chemistry-based enrichments with magnetic alkyne agarose beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD036886 | Pride
Automation Silac Click chemistry Nascent proteome 
ProteomicsApplying plexDIA analysis to newly synthesized proteome samples generated using a semi-automated enrichment protocol
Samples generated with a newly development semi-automated enrichment protocol for newly synthesized proteins, using different amounts of protein input, were analysed using data-independent acquisition (DIA) and processed with the plexDIA software features of DIA-NN (https://www.nature.com/articles/s...
ORGANISM(S): Homo sapiens (Human) 
2023-10-19 | PXD043817 | Pride
Silac Plexdia Quanpa 
ProteomicsFishing for newly synthesized proteins with phosphonate-handles
Bioorthogonal chemistry introduces affinity-labels into biomolecules with minimal disruption to the original system and is widely applicable in a range of contexts. In proteomics, immobilized metal affinity chromatography (IMAC) enables enrichment of phosphopeptides with extreme sensitivity and sele...
ORGANISM(S): Homo sapiens (Human) 
2020-07-28 | PXD018272 | Pride
Bioorthogonal Affinity enrichment Lc-ms Click chemistry 
ProteomicsSystematic and Quantitative Investigation of Newly Synthesized Proteins Reveals Distinct Ion Homeostasis and Mitochondrial Changes between Cuproptosis and Ferroptosis in Human Cells
To better understand ferroptosis and cuproptosis, here we systematically and quantitatively analyzed newly synthesized proteins (NSPs), which reflect rapid changes in gene expression during cell death without the interference from the existing proteins. Integrating metabolic labeling with multiplexe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-11-13 | MSV000099889 | MassIVE
ProteomicsNewly Synthesized protein after VapC5 induction in Mycobacterium abscessus ATCC19977
Analyzing newly synthesized protein after VapC5 induction in mycobacterium abscessus 19977
ORGANISM(S): Mycobacteroides abscessus subsp. abscessus 
2023-07-20 | PXD025047 | Pride
Lc-ms Mycobacterium abscessus atcc19977 
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