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MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsCURTAIN – A Unique Web-based tool for exploration and sharing of MS-based proteomics data
To facilitate analysis and sharing of mass spectrometry (MS)-based proteomics data we created two tools called CURTAIN (https://curtain.proteo.info) and CURTAIN-PTM (https://curtainptm.proteo.info). They are designed to enable the non-MS expert to interactively pursue volcano plots, deconvolute prim...
ORGANISM(S): Homo sapiens (Human) 
2023-12-21 | PXD043806 | Pride
Bioinformatics Ptm Bromotag Proteomics 
ProteomicsGolgi-IP, a novel tool for multimodal analysis of Golgi molecular content
The Golgi plays an essential role in protein and lipid glycosylation. Golgi dysfunction is associated with a variety of diseases including cancer, neurodegeneration and cardiovascular disease. We developed a powerful immunoprecipitation technique to isolate the Golgi by tagging TMEM115. Golgi-immuno...
ORGANISM(S): Homo sapiens (Human) 
2023-04-24 | PXD038046 | Pride
Golgi-ip Proteomics Metabolomics Lipidomics Golgi apparatus 
UnknownCURTAIN-A unique web-based tool for exploration and sharing of MS-based proteomics data.
Not available
S-EPMC10873628 | biostudies-literature
ProteomicsPKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain
Leucine-rich-repeat-kinase 1 (LRRK1) and its homologue LRRK2 are multidomain kinases possessing an atypical ROC-CORA-CORB containing GTPase domain and phosphorylate distinct Rab proteins. LRRK1 loss of function mutations cause the bone disorder osteosclerotic metaphyseal dysplasia, whereas LRRK2 mis...
ORGANISM(S): Homo sapiens (Human) 
2022-09-02 | PXD034420 | Pride
Rab7a Lrrk1 Pkc Phosphorylation 
ProteomicsDiscovery of XL01126: A Potent, Fast, Cooperative, Selective, Oral bioavailable and Blood Brain Barrier Penetrant PROTAC Degrader of Leucine Rich Repeat Kinase 2 (LRRK2)
Leucine Rich Repeat Kinase 2 (LRRK2) is one of the most promising targets for Parkinson’s Disease. LRRK2 targeting strategies have primarily focused on Type 1 kinase inhibitors, which however have limitations as the inhibited protein can interfere with natural mechanisms and lead to undesirable side...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-02 | PXD034055 | Pride
Parkinsosn's disease Tmt Lrrk2 Mass spectrometry Protac 
ProteomicsDevelopment of a multiplex targeted mass spectrometry assay to quantify levels of LRRK2 phosphorylated Rab substrates and Ser910 and Ser935 biomarker sites
Mutations that increase the protein kinase activity of LRRK2 are one of the most common causes of familial Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases within their Switch-II motif, impacting interaction with effectors. We describe and validate a new, multiplex targeted mass spe...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-03-17 | PXD022662 | Pride
Rab-gtpases Parkinson's disease Lrrk2 Prm Lc-ms/ms 
ProteomicsTagless LysoIP method for molecular profiling of lysosomal content in clinical samples
Lysosomes are implicated in a wide spectrum of human diseases including monogenic lysosomal storage disorders (LSDs), age-associated neurodegeneration and cancer. Profiling lysosomal content using tag-based lysosomal immunopurification (LysoIP) in cell and animal models allowed major discoveries in ...
ORGANISM(S): Homo sapiens (Human) 
2025-01-31 | PXD052082 | Pride
Metabolomics Proteomics Dia Lysosomal storage disorder Lipidomics Lysosome Mass spectrometry 
ProteomicsPhosphoproteomic analysis of breast epithelial cells with isogenic knockin of PIK3CA mutations
The PIK3CA gene is frequently mutated in human cancers. To study the signaling mechanisms responsible for cell growth and invasion phenotypes induced by mutant PIK3CA molecules, we carried out a SILAC-based quantitative phosphoproteomic analysis of MCF10A, a spontaneously immortalized normal mammary...
ORGANISM(S): Homo sapiens (Human) 
2015-07-28 | PXD000599 | Pride
Breast cancer Ls-ms/ms Pik3ca Phosphorylation Proteome Mutation 
ProteomicsProtein aggregation and calcium dysregulation are the earliest hallmarks of familial Parkinson’s disease in human midbrain dopaminergic neurons
Mutations in the SNCA gene cause autosomal dominant Parkinson’s disease (PD), with progressive loss of dopaminergic neurons in the substantia nigra, and accumulation of aggregates of α-synuclein. However, the sequence of molecular events that proceed from the SNCA mutation during development, to its...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD035500 | Pride
Dopaminergic neurons Proteomics and parkinsons's disease Hipscs Alpha synuclein 
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