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ProteomicsComprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb
This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb, a reference material of humanized IgG1k monoclonal antibody (RM 8671). Analyses involved both partially-reduced and non-reduced samples under neutral and w...
ORGANISM(S): Homo sapiens (Human) 
2021-02-08 | PXD023358 | Pride
Disulfide-linked peptides Disulfide Nistmab Cysteine Mass spectral library Disulfide bond 
UnknownQualification of NISTmAb charge heterogeneity control assays.
Not available
S-EPMC5830499 | biostudies-literature
UnknownDevelopment of orthogonal NISTmAb size heterogeneity control methods.
Not available
S-EPMC5830496 | biostudies-literature
UnknownThe NISTmAb Reference Material 8671 lifecycle management and quality plan.
Not available
S-EPMC5830479 | biostudies-literature
UnknownBackbone and side-chain resonance assignments of the NISTmAb-scFv and antigen-binding study.
Not available
S-EPMC9510101 | biostudies-literature
UnknownHeterologous recombinant expression of non-originator NISTmAb.
Not available
S-EPMC6152460 | biostudies-literature
UnknownDevelopment of an LC-MS/MS peptide mapping protocol for the NISTmAb.
Not available
S-EPMC5830484 | biostudies-literature
UnknownThe NISTmAb Reference Material 8671 value assignment, homogeneity, and stability.
Not available
S-EPMC5830482 | biostudies-literature
UnknownThe NISTmAb tryptic peptide spectral library for monoclonal antibody characterization.
Not available
S-EPMC5916550 | biostudies-literature
ProteomicsMulti-level – Intact, Subunits, and Peptides – Characterization of Antibody-Based Therapeutics by a Single-Column LC-MS setup
A comprehensive characterization of biotherapeutics, mandated by regulatory authorities, require analyses of a protein drug at multiple structure levels. Such multi-level characterization can be performed by mass spectrometry (MS), with established conventional MS-based assays of product quality att...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-02-26 | PXD054948 | Pride
Subunits Intact Lc-ms Ead C4-column Peptides 
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