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ProteomicsPISA Assay of B1 antibiotic on Neisseria gonorrhoeae
Study on antibiotic B1 and for target identification and mode of action characterization on Neisseria gonorrhoeae Strain, using Proteome Integral Solubility Alteration (PISA) Assay
ORGANISM(S): Neisseria gonorrhoeae 
2026-01-19 | PXD063107 | Pride
Proteomics Lc-ms Mode of action Antibiotic Target id Bacteria Pisa assay 
ProteomicsOne-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification
Proteome Integral Solubility Alteration (PISA) assay is widely used for identifying drug targets, but it is labour-, sample and time-consuming. Aiming at enabling automation and greatly reducing the sample amount, we developed One-Pot Time-Induced (OPTI)-PISA. Here we demonstrate OPTI-PISA performan...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050241 | Pride
Drug target Proteome integral solubility alteration (pisa) One pot Automation 
ProteomicsMultifaceted proteome analysis at solubility, redox, and expression dimensions for target identification
Multifaceted interrogation of the proteome deepens the system-wide understanding of biological systems; however, mapping the redox changes in the proteome has so far been significantly less informative than expression and solubility/stability analyses. Here, we devise the first high-throughput redox...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD042967 | Pride
Multidimensional Proteomics Reactive oxygen species Mass spectrometry Interferon Stability 
ProteomicsThermoTargetMiner: Proteome Integral Solubility Alteration (PISA) target database for prospective lung cancer drug candidates
Knowledge of the targets of therapeutic compounds helps understanding the compound action mechanism as well as the side-effects. The multiple complementary techniques needed for comprehensive target characterization must combine data reliability with sufficient analysis throughput. Here, we test the...
ORGANISM(S): Homo sapiens (Human) 
2026-06-08 | PXD054158 | Pride
Human Proteomics Lc-ms Lung cancer 
ProteomicsIdentifying Ceramide Protein Interactions using PISA
Ceramides are potent influencers of cell fate and function; however, their direct molecular target(s) and mechanism of action are poorly understood. In this study, we treated primary proximal tubule epithelial cells with C2-ceramide (50uM) or its precursor C2-dihydroceramide (50uM) and used proteome...
ORGANISM(S): Mus musculus (Mouse) 
2026-06-08 | PXD067617 | Pride
Ptec Mouse Pisa 
ProteomicsTowards sensitive, streamlined high throughput protein thermal stability analysis for low sample input
This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-06 | PXD061105 | Pride
Pisa lc-ms proteomics 
ProteomicsSelection of Heating Temperatures Improves the Sensitivity of Proteome Integral Solubility Alteration (PISA) Assay
The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput has been advanced greatly by the rapid progress in tandem ma...
ORGANISM(S): Homo sapiens (Human) 
2020-04-06 | PXD017346 | Pride
Proteome integral solubility alteration (pisa) assay Human cell line lysate Lc-ms/ms 
ProteomicsProteome integral solubility alteration assay for monitoring antibody-antigen interactions in cells
Proteome Integral Solubility Alteration (PISA) assay is normally used for characterization of the interactions between a small molecule and the cellular proteome. Here we modified PISA uniquely to identify the antigen (tumor necrosis factor alpha) in activated monocytes using the monoclonal antibody...
ORGANISM(S): Homo sapiens (Human) 
2026-05-20 | PXD065457 | Pride
Pisa Antibody Antigen 
ProteomicsProteome integral solubility alteration high-throughput proteomics assay identifies Collectin-12 as a non-apoptotic microglial caspase-3 substrate.
Caspases are a family of proteins mostly known for their role in the activation of the apoptotic pathway leading to cell death. In the last decade, caspases have been found to fulfil other tasks regulating the cell phenotype independently to cell death. Microglia are the immune cells of the brain re...
ORGANISM(S): Mus musculus (Mouse) 
2023-05-10 | PXD032835 | Pride
Caspase-3 Solubility Pisa Non-apoptotic Sustrates Colec12 Proteomics Microglia 
ProteomicsTowards sensitive, streamlined high throughput protein thermal stability analysis for low sample input
This method development aims to enhance and streamline excisting PISA workflows by reducing pipetting steps and digestion method. With this workflow we want to provide a method with higher reproducibility and simplicity. This method combines the excisting OneTip protocol with low cell concentration ...
ORGANISM(S): Homo sapiens (Human) 
2025-10-06 | PXD056894 | Pride
Lc-ms Onetip Method development Proteomics Pisa 
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