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MetabolomicsSerum protein N-glycosylation changes in multiple myeloma

BACKGROUND: Multiple myeloma is characterized by clonal proliferation of malignant plasma cells in the bone marrow that produce monoclonal immunoglobulins. N-glycosylation changes of these monoclonal immunoglobulins have been reported in multiple myeloma, but previous studies on...

2022-04-27 | MTBLS676 | MetaboLights
MetabolomicsTranscript, protein and metabolite temporal dynamics in the CAM plant Agave
Already a proven mechanism for drought sustainability, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency (WUE) via an inverse (compared to C3 and C4 photosynthesis-performing species) day/night pattern of stomatal closure/opening to shift ...
2017-10-13 | MTBLS363 | MetaboLights
ProteomicsC-terminomics E. coli - C-terminal charge-reversal derivatization and parallel use of multiple proteases facilitates identification of protein C-termini by C-terminomics
The identification of protein C-termini in complex proteomes is challenging due to the poor ionization efficiency of the carboxyl group. Amidating the negatively charged C-termini with ethanolamine (EA) has been suggested to improve detection of C-terminal peptides and allows for a directed depletio...
ORGANISM(S): Escherichia coli 
2016-03-11 | PXD003342 | Pride
Cid Hcd Etd C-terminus Derivatization 
ProteomicsHydrophobic derivatization strategy facilitates comprehensive profiling of protein methylation
Hydrophobic derivatization strategy facilitates comprehensive profiling of protein methylation
ORGANISM(S): Homo Sapiens 
2023-08-12 | PXD044539 |
ProteomicsMicrowave oven-assisted derivatization of histone proteoforms using trimethylacetic anhydride
We introduce a new reagent – Trimethylacetic anhydride (TMA) for highly efficient histone derivatization, which is facilitated by microwave irradiation to reduce sample preparation duration. The derivatization of unmodified NH2- groups at histone lysine residues is necessary prior LC-MS analysis usi...
ORGANISM(S): Homo sapiens (Human) 
2021-06-14 | PXD019502 | Pride
O histone derivatization Epigenetics Mass spectrometry Trimethylacetic anhydride 
ProteomicsN-Terminal Protein Sequencing by Nicotinic Acid Derivatization and MS Analysis
Most MS-based workflows for N-terminal protein sequence analysis rely on the modification of primary amines at the protein N-terminus and lysine residues, in many cases through dimethylation. However, incomplete dimethyl labeling and undesired side reactions have been reported previously and were al...
ORGANISM(S): Bos taurus (Bovine) Escherichia coli Equus caballus (Horse) 
2026-08-17 | PXD081064 | Pride
Protein sequencing Dimethylation N-terminomics Nicotinic acid N-terminal 
ProteomicsA Derivatization-Free PRM Workflow for Quantitative, Site-Specific Analysis of Histone Post-Translational Modifications
Histone post-translational modifications (PTMs) are key regulators of chromatin architecture and gene expression. Although DIA-based platforms for histone PTM quantification are available, multiplexed targeted assays remain underdeveloped. In addition, bottom-up workflows frequently rely on chemical...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2026-09-09 | PXD071393 | Pride
Ptms Prm Skyline Quantitation Histone Argc 
UnknownProtein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids.
Not available
S-EPMC4983383 | biostudies-literature
ProteomicsReversible Lysine Derivatization Enables High Specificity of Arg-C Digestion Using Trypsin
Bottom-up proteomics approach has become an important strategy in diverse areas of biological research, and the enzymatic digestion is essential for this technology. Endopeptidase Arg-C catalyzing the hydrolytic cleavage of peptide bonds C-terminal to arginine could be an important protease in botto...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-01-04 | PXD007994 | Pride
Proteomics Arg-c Trypsin Lys-c Citraconylation Lc-ms/ms 
ProteomicsArg-C Ultra simplifies histone preparation for LC-MS/MS
Using mammalian histone extract, we demonstrate that Arg-C Ultra facilitates histone preparation for LC-MS/MS. We show the performance of Arg-C Ultra in terms of digestion specificity, number of modified forms identified, and yield of quantitative information compared with standard Arg-C and trypsin...
ORGANISM(S): Homo sapiens (Human) 
2025-06-30 | PXD059514 | Pride
Histone Arg-c ultra Trimethylacetic anhydride Lc-ms/ms Chemical derivatization 
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