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ProteomicsA sensitive and cost-effective approach for tyrosine phosphoproteome analysis based on SH2 superbinder
Albeit much less abundant than Ser/Thr phosphorylation (pSer/pThr), Tyr phosphorylation (pTyr) is considered a hallmark in cellular signal transduction. However, its analysis remains a challenge. The conventional immunopurification (IP) approach using antibodies pan-specific to pTyr sites is known t...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-08-29 | PXD005838 | Pride
Lc ms/ms Sh2 superbinder Ptyr phosphoproteome 
ProteomicsTandem Mass Tag approach utilizing pervanadate BOOST channels delivers deeper quantitative characterization of the tyrosine phosphoproteome
Dynamic tyrosine phosphorylation is fundamental to a myriad of cellular processes. However, the inherently low abundance of tyrosine phosphorylation in the proteome and the inefficient enrichment of phosphotyrosine(pTyr)-containing peptides has led to poor pTyr peptide identification and quantitati...
ORGANISM(S): Homo sapiens (Human) 
2020-03-24 | PXD016192 | Pride
Superbinder Trigger channel Phosphotyrosine proteomics Tandem mass tags Pervanadate 
UnknownEngineered SH2 domains with tailored specificities and enhanced affinities for phosphoproteome analysis.
Not available
S-EPMC6319756 | biostudies-literature
ProteomicsProteome and phosphoproteome signatures of recurrence for HPV+ head and neck squamous cell carcinoma
Head and neck squamous cell carcinoma (HNSCC) is the 6th most common cancer worldwide and the human papillomavirus (HPVP+P)-driven subtype is the fastest rising in North America. Although most cases of HPV+ HNSCC respond to radiation and chemotherapy in combination with surgery, up to 20% of patient...
ORGANISM(S): Homo sapiens (Human) 
2022-06-01 | PXD030343 | Pride
Proteomics Mass spectrometry Recurrence Human papillomavirus Fibrosis Phosphoproteomics Tumor microenvironment Immune landscape Head and neck squamous cell carcinoma 
Proteomics[HPP-2.0] Integrated and high-throughput approach for sensitive analysis of tyrosine phosphoproteome
In this study, we developed a 96-well microplate-based approach to integrate all the sample preparation steps starting from cell culture to MS-compatible pTyr peptide enrichment in three consecutive 96-well microplates. By assembling engineered SH2 domain onto microplate, non-specific adsorption of ...
ORGANISM(S): Homo Sapiens 
2024-02-03 | PXD049140 |
UnknownTandem Mass Tag Approach Utilizing Pervanadate BOOST Channels Delivers Deeper Quantitative Characterization of the Tyrosine Phosphoproteome.
Not available
S-EPMC7124467 | biostudies-literature
ProteomicsKinome and phosphoproteome reprogramming underlie the immunological abnormalities in severe COVID-19
The SARS-CoV-2 infection elicits widespread immunological reactions and causes severe diseases in some individuals. However, the molecular basis behind the excessive, yet non-productive immune response in COVID-19 patients with severe diseases is not fully understood. Nor is it fully known of the mo...
ORGANISM(S): Homo sapiens (Human) 
2024-05-24 | PXD024087 | Pride
T cell Immune regulation Phosphoproteome Inhibitory receptor Ck2 Sars-cov-2 Il-12 Syk Covid-19 Cytokine Natural killer cells Immunoreceptor tyr-based motifs Signal transduction B cell Kinase Jak-stat Proteome 
UnknownKinase Inhibitor Treatment of Patients with Advanced Cancer Results in High Tumor Drug Concentrations and in Specific Alterations of the Tumor Phosphoproteome.
Not available
S-EPMC7072422 | biostudies-literature
ProteomicsLIN28A-Dependent Kinome and Phosphoproteome Reprogramming Promotes Imatinib Resistance
Chronic myeloid leukemia (CML) resistance to BCR-ABL tyrosine kinase inhibitors (TKIs) can arise from ABL kinase domain mutations, BCR-ABL fusion gene amplification, or kinase-independent mechanisms. To investigate imatinib-resistance, we performed quantitative mass spectrometry comparing the proteo...
ORGANISM(S): Homo sapiens (Human) 
2026-01-22 | PXD066618 | Pride
Imatinib K562 Tki resistance Lin28a Phosphoproteomics 
ProteomicsPhosphoproteomics informed tailored low-dose drug combination strategies in Pancreatic ductal adenocarcinoma cell lines
Pancreatic ductal adenocarcinoma is a devastating disease with poor prognosis, commonly characterized by aberrant signaling. Phosphoproteomics provides a functional scaffold to unravel these complex signaling networks and to identify new targets. By a two-step sequential phospho-peptide enrichment, ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-09 | PXD036951 | Pride
Pdac Imac Single shot Kinase Phosphoproteomics Ptyr ip Inka 
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