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ProteomicsThermal proteome profiling of cell cycle
We combined quantitative mass spectrometry with thermal profiling to systematically analyze the thermal stability and solubility of proteins during the eukaryotic cell cycle on a proteome-wide scale.
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008646 | Pride
Complexes Disordered proteins Cell cycle Thermal stability 
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsThermal proteome profiling in Escherichia coli
We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-07-09 | PXD009495 | Pride
Target engagement Escherichia coli Drugs Growth phase Meltome Thermal proteome profiling 
ProteomicsThermal proteome profiling of temporal dynamics of infection of SARS-CoV-2
We performed two dimensional thermal proteome profiling (2D-TPP) to profile the infection dynamics of SARS-CoV-2 infection in Caco-2 cells.
ORGANISM(S): Homo sapiens (Human) 
2020-11-23 | PXD022115 | Pride
Virus Sars-cov-2 Infection Coronavirus Thermal proteome profiling 
ProteomicsThermal proteome profiling of yeast phosphodeficient mutants
We performed two dimensional thermal proteome profiling (2D-TPP) of phosphodeficient mutantsof Saccharomyces cerevisiae.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-09-17 | PXD017929 | Pride
Saccharomyces cerevisiae Thermal proteome profiling Phosphomutants 
ProteomicsThermal proteome profiling of darobactin in Escherichia coli
We performed two dimensional thermal proteome profiling (2D-TPP) of darobactin to study its impact in Escherichia coli.
ORGANISM(S): Escherichia coli 
2019-10-29 | PXD013319 | Pride
Target engagement Darobactin Escherichia coli Drugs Thermal proteome profiling 
ProteomicsThermal proteome profiling SCH79797 and trimethoprim in Escherichia coli
We performed thermal proteome profiling (TPP) to identify drug targets of SCH79797 and trimethoprim in Escherichia coli.
ORGANISM(S): Escherichia coli 
2020-05-19 | PXD013673 | Pride
Target engagement Escherichia coli Drugs Thermal proteome profiling 
ProteomicsPhosphoProtein-Peptide Turnover Profiling, exploratory
We combine pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We apply the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using th...
ORGANISM(S): Homo sapiens (Human) 
2022-12-02 | PXD033254 | Pride
Post-translational modification Protein maturation Protein turnover Phosphorylation Mass spectrometry 
ProteomicsPhosphoProtein-Peptide Turnover Profiling, validation
We combined pulsed stable isotope labeling of amino acids (pSILAC) with enrichment of phosphorylated peptides in a method we term PhosphoProtein-Peptide Turnover Profiling (PhosphoPPToP). We applied the method to HeLa cells to find peptides whose clearance rates differ from the protein median. Using...
ORGANISM(S): Homo sapiens (Human) 
2022-12-02 | PXD032945 | Pride
Ap-ms Post-translational modification Protein maturation Gfp-fusion Pull-down Silac Protein turnover Phosphorylation Mass spectrometry Tmt16 
ProteomicsSubcellular proteomics of murine ESC-derived neurons
We studied the effects of different stimuli on on the subcellular proteome of neurons differentiated from murine embryonic stem cells (ESC). We focused on the changes in the chromatin-bound fraction to elucidate specific differences in the gene regulatory machinery activated upon stimulation with Br...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-15 | PXD022378 | Pride
Neurons Stimulation Subcellular fractionation Tmt Bdnf Kcl Thermo q exactive plus 
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