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ProteomicsA study into the ADP-ribosylome of IFN--stimulated THP-1 human macrophage-like cells identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-ribosylation
ADP-ribosylation is a post-translational modification that, until recently, has remained elusive to study at the cellular level. Previously dependent on radioactive tracers to identify ADP-ribosylation targets, several advances in mass spectrometric workflows now permit global identification of ADP-...
ORGANISM(S): Homo sapiens (Human) 
2020-01-27 | PXD011690 | Pride
Electron transfer higher-energy collision dissociation (ethcd) Proteomics Gas phase segmentation Higher-energy collision dissociation (hcd) Parallel reaction monitoring (prm) Orbitrap fusion lumos Post-translational modification (ptm) 
ProteomicsMulti-organ systems study reveals Igfbp7 as a suppressor of gluconeogenesis after gastric bypass surgery
Roux-en-Y gastric bypass (RYGB) surgery reduces weight in obese patients. A marked decrease in blood glucose levels occurs before weight loss; however, key molecules that improve glycemic profile remain largely unknown. We used the RYGB surgery model in diet-induced obese (DIO) mice to monitor the p...
ORGANISM(S): Mus musculus (Mouse) 
2020-01-23 | PXD015276 | Pride
Diabetes Bariatric surgery Insulin-like growth factor binding protein 7 Metabolic syndrome Protein-protein interactions Obesity Proteomics Tandem mass tagging 
ProteomicsModel-Specific Immune Signatures of Human Macrophages: Insights from Proteomic and Functional Profiling
The human leukemia monocytic cell line, THP-1, is widely used as an in vitro model to study human macrophage biology. Recently, the B cell leukemia cell line, BLaER1, was proposed as an alternative cell model for macrophages. Despite their broadscale use, the molecular and functional fidelity to pri...
ORGANISM(S): Homo sapiens (Human) 
2026-10-27 | PXD070966 | Pride
Pbmc Mitochondrial function Ifnγ Thp-1 Mass spectrometry Blaer1 Il-4 
ProteomicsA combined gas phase separation strategy for ADP-ribosylated peptides
ADP-ribosylation (ADPr) is a posttranslational modification that is best studied using mass spectrometry. Method developments that are permissive to low inputs or baseline levels of protein ribosylation represent the next frontier in the field. High-field asymmetric waveform ion mobility spectrometr...
ORGANISM(S): Mus musculus (Mouse) 
2023-08-30 | PXD040898 | Pride
Mono-adp-ribose Gas-phase segmentation Ribosylation High field asymmetric waveform ion mobility spectrometry Gas-phase fragmentation 
ProteomicsComputational Prediction, Validation and Repurposing of Existing Drug as Inhibitor of Vascular Calcification
This study aimed to identify small molecules that inhibit vascular calcification with computational approach. To examine the mechanism of calcification inhibition, proteomics analysis was performed using aorta tissue samples to clarify therapeutic target.
ORGANISM(S): Mus musculus (Mouse) 
2022-02-17 | PXD029963 | Pride
Vascular calcification Proteomics Mice Wnt signaling Drug discovery Drug repurposing 
ProteomicsAn evaluation of high-field asymmetric-waveform ion mobility spectrometry coupled to electron-transfer/higher-energy collision dissociation for ADP-ribosylation proteomics
Objective: ADP-ribosylation is a post-translational modification that plays an important role in cellular processes. Our previous work implemented multiple gas-phase separation strategies (e.g., FAIMS) and in-source CID on the quadrupole-Orbitrap (Exploris 480) to increase the yield and acceptor sit...
ORGANISM(S): Homo sapiens (Human) 
2026-04-13 | PXD067389 | Pride
Macrophage Proteomics Ethcd Hcd Adp-ribosylation 
ProteomicsNanoparticle-enabled plasma proteomics of a preclinical atherosclerosis model reveals common signatures with human disease
Coronary heart disease (CHD) accounts for one-third of deaths in the United States, with an annual economic burden exceeding $400 billion. Elevated plasma levels of low-density lipoprotein-cholesterol (LDL-C) is a major risk factor for CHD, through its promotion of atherosclerosis. Mutations in the ...
ORGANISM(S): Mus musculus (Mouse) 
2026-08-20 | PXD063218 | Pride
Plasma Atherosclerosis Dia Nanoparticle Proteomics 
ProteomicsHuman iPSCs differentiated into SMCs
Human iPSCs were differentiated towards an induced-SMC (iSMC) phenotype in a 10-day protocol. Proteomics was performed throughout the entire differentiation time course to provide a robust, well-defined starting and ending cell population. Proteomics data verified iPSC differentiation to iSMCs. Prot...
ORGANISM(S): Homo sapiens (Human) 
2022-10-14 | PXD032353 | Pride
Calcification Smooth muscle cell Lumos 
ProteomicsA Novel Spectral Annotation Strategy Streamlines Reporting of mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-gamma
Mass spectrometry-enabled ADP-ribosylation workflows are developing rapidly, providing researchers a variety of ADP-ribosylome enrichment strategies and mass spectrometric acquisition options. Despite the growth spurt in upstream technologies, systematic ADP-ribosyl (ADPr) peptide mass spectral anno...
ORGANISM(S): Mus musculus (Mouse) 
2021-11-02 | PXD027454 | Pride
Ifn-gamma Spleen Software Liver Adp-ribosylation Lc-ms/ms 
ProteomicsMouse and human abdominal aneurysm proteomics
Aortic tissues of developing and established aneurysms produced by angiotensin II (AngII) infusion were examined in Apoe-/- and Ldlr-/- mice. Aortas were also acquired from wildtype C57BL/6J mice following intraluminal elastase incubation. Aortas were dissected free and separated into eight anatom...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2022-08-02 | PXD032293 | Pride
Fermt3 Co-regulation Mass spectrometry 
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