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MetabolomicsThermal Profiling Maps Multilayered Organization of the Mitochondrial Proteome

Mitochondria execute essential metabolic, biosynthetic, and signaling functions, yet the regulatory principles governing their proteome remain incompletely understood. Here, we develop a thermal proteome profiling (TPP) workflow applied directly to purified mitochondria, enabling systematic analy...

2025-12-08 | MTBLS7386 | MetaboLights
ProteomicsThermal proteome profiling on zebrafish using napabucasin
In this project we performed thermal proteome profiling experiments on zebrafish embryos with the drug napabucasin. The lysate was heat treated, TMT labelled and subsuquently high pH fractionated.
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2020-10-29 | PXD017419 | Pride
Thermal proteome profiling Tmt Zebrafish 
ProteomicsNonionic surfactants can modify the thermal stability of globular and membrane proteins interfering with the thermal proteome profiling principles to identify protein targets
The membrane proteins are essential targets to understand cellular function. The unbiased identification of membrane protein targets is still the bottleneck for a system- level understanding of cellular response to stimuli or perturbations. It has been suggested to enrich the soluble proteome with m...
ORGANISM(S): Rattus norvegicus (Rat) 
2023-03-20 | PXD037153 | Pride
Rat liver tissue Igepal Micelles Nonionic surfactants Thermal proteome profiling Nonidet-p40 substitute 
ProteomicsThermal proteome profiling of phosphorylated proteoforms
We combined thermal proteome profiling (TPP) with phosphopeptide enrichment to study the thermostability of phosphorylated proteoforms.
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD015993 | Pride
Phosphopeptides Meltome Phosphoproteomics Thermal proteome profiling 
ProteomicsThermal proteome profiling in Escherichia coli
We adapted thermal proteome profiling (TPP) to study the thermostability of Escherichia coli proteins in vivo. We monitored the E. coli meltome and proteome at different growth phases, in a tolC knock-out mutant and after drug treatment.
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2018-07-09 | PXD009495 | Pride
Target engagement Escherichia coli Drugs Growth phase Meltome Thermal proteome profiling 
ProteomicsOff-target profiling of Olaparib using Thermal Proteome Profiling
Explore off-targets of Olaparib using 2D Thermal Proteome Profiling (2D-TPP)
ORGANISM(S): Homo sapiens (Human) 
2024-05-13 | PXD046614 | Pride
Thermal proteome profiling Parp 2d-tpp Olaparib 
ProteomicsDanio rerio thermal proteome profiling with pervanadate as drug of interest
In this study we performed thermal proteome profiling experiments on zebrafish lysate after treatment with the drug pervanadate.
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2020-10-29 | PXD017418 | Pride
High ph Tmt Danio rerio Thermal proteome profiling 
ProteomicsThermal proteome profiling of cell cycle
We combined quantitative mass spectrometry with thermal profiling to systematically analyze the thermal stability and solubility of proteins during the eukaryotic cell cycle on a proteome-wide scale.
ORGANISM(S): Homo sapiens (Human) 
2018-05-02 | PXD008646 | Pride
Complexes Disordered proteins Cell cycle Thermal stability 
ProteomicsThermal proteome profiling and proteome analysis using high-definition mass spectrometry demonstrate modulation of cholesterol biosynthesis by next-generation galeterone analog VNPP433-3β in castration-resistant prostate cancer
Cholesterol (CHOL) homeostasis is significantly modulated in prostate cancer (PCa) suggesting an active role in PCa development and progression. Several studies indicate a strong correlation between elevated CHOL levels and increased PCa risk and severity. Inhibition of CHOL biosynthesis at differen...
ORGANISM(S): Homo sapiens (Human) 
2025-08-25 | PXD060338 | Pride
Next generation galeterone analog Cyp51a1 Thermal proteome profiling Cholesterol Prostate cancer Lanosterol synthase 
ProteomicsThermal proteome profiling of Escherichia coli mutants
We performed two dimensional thermal proteome profiling (2D-TPP) in Escherichia coli mutants to measure changes in abundance and thermal stability.
ORGANISM(S): Escherichia coli 
2020-09-11 | PXD016589 | Pride
Escherichia coli Genetic perturbation Mutants Thermal proteome profiling 
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