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MetabolomicsMulti-omics and biophysical phosphoproteomics uncover functional phosphorylation networks of BRAFV600E-driven signaling

Dysregulated kinase activity drives oncogenic signalling, perturbs cellular homeostasis, and promotes tumour progression. Despite major success in targeting kinases therapeutically, the downstream consequences of kinase inhibition and the mechanisms underlying drug resistance remain incompletely ...

2025-12-09 | MTBLS7390 | MetaboLights
ProteomicsEvaluation of phospho-tyrosine antibodies for label-free phosphoproteomics
In the past decade multiple tyrosine kinase inhibitors (TKIs) have been implemented in standard treatment regimens for patients with cancer. Unfortunately the majority of patients develops resistance to these drugs. Reliable tools for analysis of pharmacodynamic effects and drug resistance mechanism...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-10-21 | MSV000080270 | MassIVE
ProteomicsParallel phosphoproteomics and metabolomics map the global metabolic tyrosine phosphoproteome
Tyrosine phosphorylation of metabolic enzymes is an evolutionarily conserved post-translational modification that facilitates rapid and reversible modulation of enzyme activity, localization or function. Despite the high abundance of tyrosine phosphorylation events detected on metabolic enzymes in h...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD052217 | Pride
Metabolomics Egf signaling Phosphoprotoemics Tyrosine phosphorylation 
ProteomicsTyrosine phosphoproteomics of patient-derived xenografts reveals ephrin type-B receptor 4 tyrosine kinase as novel therapeutic target in pancreatic cancer
Pancreatic ductal adenocarcinoma is a recalcitrant tumor with minimal response to conventional chemotherapeutic approaches. Oncogenic signaling by activated tyrosine kinases has been implicated in cancers resulting in activation of diverse effector signaling pathways. Thus, discovery of aberrantly a...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2021-05-24 | PXD021729 | Pride
Receptor tyrosine kinase Ptyr Pdx Signaling Cancer 
Proteomics[HPP-2.0] A tyrosine phosphoproteome analysis approach enabled by selective dephosphorylation with protein tyrosine phosphatase
Protein tyrosine phosphorylation (pTyr) plays a prominent role in signal transduction and regulation in all eukaryotic cells. In conventional immunoaffinity purification (IP) methods, phosphotyrosine peptides are isolated from the digest of cellular protein extracts with a phosphotyrosine-specific a...
ORGANISM(S): Homo Sapiens 
PXD045682 |
ProteomicsEvaluation of phospho-tyrosine antibodies for label-free phosphoproteomics
In the past decade multiple tyrosine kinase inhibitors (TKIs) have been implemented in standard treatment regimens for patients with cancer. Unfortunately the majority of patients develops resistance to these drugs. Reliable tools for analysis of pharmacodynamic effects and drug resistance mechanism...
ORGANISM(S): Homo sapiens (Human) 
2015-12-09 | PXD001565 | Pride
Human Glioblastoma Single-shot Label-free U87 Phosphoproteomics Phosphotyrosine Ptyr ip 
ProteomicsPhosphoproteomics analysis identifies novel candidate substrates of the non-receptor tyrosine kinase, SRMS
SRMS (Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) or PTK 70 (Protein tyrosine kinase 70) is a non-receptor tyrosine kinase that belongs to the BRK family kinases (BFKs). The gene encoding SRMS was first discovered in 1994. Yet to date less is known ...
ORGANISM(S): Homo sapiens (Human) 
2018-03-08 | PXD006809 | Pride
Frk Srms Substrates Brk Mass spectrometry Ptk6 Src Phosphoproteomics Ptk 70 
ProteomicsLarge-scale phosphoproteomics reveals Shp-2 phosphatase-dependent regulators of Pdgf-receptor signaling
Despite its low cellular abundance, tyrosine phosphorylation (pTyr) regulates numerous cell signaling pathways in health and disease. We applied comprehensive phosphoproteomics to unravel differential regulators of receptor tyrosine kinase (RTK)–initiated signaling networks upon activation by Pdgf-β...
ORGANISM(S): Mus musculus (Mouse) 
2018-03-07 | PXD005803 | Pride
Shp099 Q-exactive Tyrosine phosphorylation Tio2 Mass spectrometry Phosphoproteomics Label-free quantitation Pdgf Shp-2 Orbitrap 
ProteomicsQuantitative tyrosine phosphoproteomics of wild type and PTPRK KO tumour xenografts
TMT-based quantitation of tyrosine phosphoproteomes of wild type and PTPRK KO tumour xenografts to determine additional mechanisms of tumour suppression by receptor tyrosine phosphatase PTPRK
ORGANISM(S): Homo sapiens (Human) 
2024-07-03 | PXD050617 | Pride
Tmt Phosphatase Ptprk Tyrosine phosphoproteomics Tumour xenografts 
ProteomicsProbing tyrosine kinase inhibitors specificity
Using a three-pronged MS-based proteomic approach, we assessed the mode-of-action of four clinically-relevant tyrosine kinase inhibitors in an epithelial cancer cell line. Here we defined the: (I) semi-quantitative tyrosine kinome; (II) drug interactome; and (III) drug-induced effects on the tyrosin...
ORGANISM(S): Homo sapiens (Human) 
2014-05-12 | PXD000681 | Pride
Human Tyrosine kinase inhibitors Chemical proteomics Phosphoproteomics Phosphotyrosine Lc-ms/ms 
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