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ProteomicsCharacterisation of protein ubiquitination using UbiSite technology
We present a novel immune-affinity-based method UbiSite for capture and further identification of ubiquitination sites by MS for any cell line or tissue sample. Implementing the UbiSite methodology for a large-scale experiment we successfully mapped 62000 unique ubiquitination sites on protein subst...
ORGANISM(S): Homo sapiens (Human) 
2018-07-03 | PXD006201 | Pride
Ubisite  site specific ubiquitination  ubiquitination sites  n-terminal ubiquitination  proteasomal inhibition  b-ap15  bortezomib 
ProteomicsCharacterisation of protein ubiquitination using UbiSite technology
We present a novel immune-affinity-based method UbiSite for capture and further identification of ubiquitination sites by MS for any cell line or tissue sample. Implementing the UbiSite methodology for a large-scale experiment we successfully mapped 62000 unique ubiquitination sites on protein subst...
ORGANISM(S): Homo sapiens (Human) 
2018-07-03 | PXD006299 | Pride
Ubisite  site specific ubiquitination  ubiquitination sites  n-terminal ubiquitination  proteasomal inhibition  b-ap15  bortezomib 
ProteomicsCharacterisation of protein ubiquitination using UbiSite technology
We present a novel immune-affinity-based method UbiSite for capture and further identification of ubiquitination sites by MS for any cell line or tissue sample. Implementing the UbiSite methodology for a large-scale experiment we successfully mapped 62000 unique ubiquitination sites on protein subst...
ORGANISM(S): Homo sapiens (Human) 
2018-07-10 | PXD009158 | Pride
Q exactive Proteome Human Jurkat Hep2 Dia 
ProteomicsDeubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.
The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinom...
ORGANISM(S): Homo sapiens (Human) 
2022-05-23 | PXD027328 | Pride
Human Ubisite Pr619 Tak243 U2os Ubiquitin Mg132 
ProteomicsThe inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation
In the project « The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation » Jianwen Zhou, Nikoline Lander Rasmussen, Vyacheslav Akimov, Blagoy Blagoev, Trond Lamark, Terje Johansen and Jörn Dengjel performed MS-based proteomics to study the f...
ORGANISM(S): Homo sapiens (Human) 
2022-08-07 | PXD027163 | Pride
Lc-msms Inflammation Silac Human 
ProteomicsDeubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.
The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinom...
ORGANISM(S): Homo sapiens (Human) 
2022-05-23 | PXD030644 | Pride
Dia The ubiquitin-proteasome system Lc-ms/ms 
ProteomicsDDI2 is a ubiquitin-directed endoprotease, responsible for cleavage of transcription factor NRF1
The Ddi1/DDI2 proteins are ubiquitin shuttling factors, implicated in a variety of cellular functions. In addition to ubiquitin-binding and ubiquitin-like domains, they contain a conserved region with similarity to retroviral proteases, but whether and how DDI2 functions as a protease has remained u...
ORGANISM(S): Homo sapiens (Human) 
2020-06-24 | PXD019152 | Pride
Ubisite Prm Ddi2 Ubiquitin Hph Lc-ms/ms 
UnknownDeubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates.
Not available
S-EPMC9117253 | biostudies-literature
ProteomicsLarge-scale profiling of unexpected tryptic cleaved sites at ubiquitinated lysines
Trypsin specifically cleaves the C-terminus of lysine and arginine residues and is assumed to fails to cleave ubiquitin-modified sites. Based on this perception, the irregularly-cleaved ubiquitinated peptides were often regarded as false positives and discarded in proteomic analysis. Interestingly, ...
ORGANISM(S): Homo Sapiens 
2023-01-10 | PXD039353 |
UnknownIdentification of ester-linked ubiquitylation sites during TLR7 signalling increases the number of inter-ubiquitin linkages from 8 to 12.
Not available
S-EPMC9788571 | biostudies-literature
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