HIS-SUMO2 expressing HeLa and U2OS cells were treated with ubiquitin E1 inhibitor TAK243 with or without the presence of cycloheximide. Subsequently, HIS-SUMO2 conjugates were purified and analyzed by LC-MS/MS
Cell cycle progression is orchestrated by complex interplay between post-translational modifications (PTMs) controlling activation (phosphorylation) and degradation (ubiquitination) of key cell cycle regulators. To comprehensively identify ubiquitin signalling components critical for cell proliferat...
We sought to comprehensively map ubiquitin signalling networks at the crossroads of cellular proliferation and proteostasis. Genome-wide CRISPR knock-out screening in the presence of the ubiquitin E1 inhibitor TAK-243 identified 239 genes whose ablation sensitizes cells to ubiquitination blockade. F...
The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinom...
The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinom...
