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ProteomicsMouse Heart SS-31 Proteome and Phosphoproteome
The heart proteome and phosphoproteome were analyzed in young (5-6-month old), old (24-month old at the start of the study), and elamipretide-treated (for 8 weeks) old mice using shotgun proteomics methods in order to assess the effects of aging on signaling and the ability of mitochondrion-targeted...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-17 | PXD026335 | Pride
Phosphoproteome Mouse Ss-31 Heart tissue Proteome Aging Elamipretide Lc-ms/ms 
ProteomicsIsobaricQuant enables cross-platform quantification, visualization, and filtering of isobarically-labeled peptides
In mass spectrometry (MS)-based quantitative proteomics, labeling with isobaric mass tags such as iTRAQ and TMT can substantially improve sample throughput and reduce peptide missing values. Nonetheless, the quantification of labeled peptides tends to suffer from reduced quantitative accuracy due to...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2022-07-11 | PXD034534 | Pride
Reanalysis 
ProteomicsAutomated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics
Phosphotyrosine (pY) enrichment is critical for expanding fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to expensive affinity reagents and manua...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD041271 | Pride
Hela Lc-msms Kingfisher Orbitrap eclipse Orbitrap exploris 480 Phosphotyrosine enrichment 
ProteomicsSialoglycans on human T cells attenuate death programs executed through the Fas pathway
T cells are critical executors of adaptive immune functions and their persistence is tightly regulated. Part of this regulation relies on programmed cell death driven by the Tumour Necrosis Factor (TNF) receptor superfamily. The addition of glycans that terminate in the monosaccharide sialic acid (s...
ORGANISM(S): Homo sapiens (Human) 
2025-12-30 | PXD068522 | Pride
Immunology Glycobiology Sialic acid Apoptosis T cells 
ProteomicsQuantification of proline to serine mistranslation in a Saccharomyces cerevisiae strain expressing an inducible tRNA variant
Altering the genetic code for applications in synthetic biology and genetic code expansion involves engineered tRNAs that incorporate amino acids that differ from what is defined by the “standard” genetic code. Since these engineered tRNA variants can be lethal due to proteotoxic stress, regulating ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-11-03 | PXD028496 | Pride
Yeast Inducible Mistranslation Trna variants 
ProteomicsIdentification of proline to serine mistranslation in Drosophila melanogaster pupae
Transfer RNAs (tRNAs) are the adaptor molecules required for reading of the genetic code and the accurate production of proteins. tRNA variants can lead to genome-wide mistranslation, the misincorporation of amino acids not specified by the standard genetic code, into nascent proteins. While genome ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-09-18 | PXD028498 | Pride
Pupae Mistranslation Trna variants Drosophila melanogaster 
ProteomicsQuantifying mistranslation from alanine tRNA anticodon variants in S. cerevisiae
Transfer RNAs (tRNAs) maintain translational fidelity through strict charging by their cognate aminoacyl-tRNA synthetase and codon:anticodon base pairing with the mRNA at the ribosome. Mistranslation occurs when an amino acid not specified by the genetic code is incorporated into a protein. Since al...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-10-02 | PXD038242 | Pride
Lc-msms Mistranslation Trna variants Yeast 
ProteomicsDetecting mistranslation from a serine tRNA with proline anticodon expressed in yeast
Mistranslation, the mis-incorporation of an amino acid not specified by the “standard” genetic code, occurs in all cells. tRNA variants that increase mistranslation arise spontaneously and engineered tRNAs can achieve mistranslation frequencies approaching 10% in yeast and bacteria. The goal of this...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD032063 | Pride
Yeast Trna Mistranslation 
ProteomicsAnalysis Of Molecular Networks In The Cerebellum In Chronic Schizophrenia: Modulation By Early Postnatal Life Stressors In Murine Models
Despite the growing importance of the cortico-cerebellar-thalamo-cortical circuit in schizophrenia, limited information is available regarding altered molecular networks in cerebellum. To identify altered protein networks, we conducted proteomic analysis of grey matter of postmortem cerebellar corte...
ORGANISM(S): Homo sapiens (Human) 
2021-12-30 | PXD024937 | Pride
Human Brain Cerebellum 
ProteomicsFeasibility of protein turnover studies in prototroph yeast
Quantitative proteomics studies of yeast that use metabolic labeling with amino acids rely on auxotrophic mutations of one or more genes on the amino acid biosynthesis pathways. These mutations affect yeast metabolism, and preclude the study of some biological processes. Overcoming this limitation, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-03-27 | PXD001823 | Pride
Yeast Protein half life Native silac Saccharomyces cerevisiae Qexactive Pulsed silac Lc-ms/ms 
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