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ProteomicsRobust, reproducible, and economical phosphopeptide enrichment using calcium titanate.
We compare different preparations of TiO2 for phosphopeptide enrichment and demonstrate the efficacy of a method using CaTiO3
ORGANISM(S): Homo sapiens (Human) 
2019-01-02 | PXD011145 | Pride
Phosphoproteomics Enrichment Phosphopeptide 
ProteomicsAutomated Phosphopeptide Enrichment for Gram Positive Bacteria
Protein phosphorylation in prokaryotes has gained more attention in recent years as several studies linked it to regulatory and signalling functions indicating an importance similar to protein phosphorylation in eukaryotes. Studies on bacterial phosphorylation have so far been conducted using manual...
ORGANISM(S): Listeria monocytogenes EGD 
2021-08-26 | PXD025459 | Pride
Bravo assaymap  fe(iii)-imac  tio2  automation  phosphopeptide enrichment  phosphoproteomics  quantification  listeria monocytogenes  bacillus subtilis  streptococcus pyogenes 
ProteomicsComprehensive evaluation of different TiO2-based phosphopeptide enrichment and fractionation methods for phosphoproteomics
We performed a comprehensive evaluation of four different TiO2-based phosphopeptide enrichment methods using different non-phosphopeptide excluders. Then, two phosphopeptide fractionation methods, including the ammonia-based and the TEA based high pH reversed-phase fractionation, are also evaluated.
ORGANISM(S): Homo Sapiens 
2022-06-02 | PXD034264 |
ProteomicsCharacterization of phosphopeptide enrichment strategy for mass spectrometry-based proteomic analysis
We attempted to evaluate four strategies for phosphopeptide enrichment, using the titanium dioxide (TiO2) and Phos-tag ligand particles, from in-solution or in-gel digests prior to mass spectrometry-based proteomic analysis.
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2021-12-24 | PXD026295 | JPOST Repository
ProteomicsPhosphopeptide enrichment on synthetic libraries and HeLa cells digest
2 phosphopeptide enrichment methods (TiO2 and Ti-IMAC) were evaluated on synthetic libraries and HeLa cells digest. The resulting peptides were compared to the ones without enrichment with regards to the nature of the phosphorylation site (serine, threonine, tyrosine) and the characteristics of the ...
ORGANISM(S): Homo sapiens (Human) 
2014-08-11 | PXD000759 | Pride
Phosphopeptide enrichment Tio2 Ti-imac Hela Synthetic library 
ProteomicsAmorphous TiO2 Nanotubes as Platform for Highly Selective Phos-phopeptide Enrichment
This work reports highly selective phosphopeptide enrichment using amorphous TiO2 nanotubes (TiO2NTs) and the same material decorated with superparamagnetic Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs). TiO2NTs and TiO2NTs@Fe3O4NPs materials were applied for phosphopeptide enrichment both from a simple pe...
ORGANISM(S): Homo sapiens (Human) 
2019-06-26 | PXD012749 | Pride
Phosphopeptide enrichment Nanotubes 
ProteomicsHuman brain phosphoproteome analyzed by refined phosphopeptide enrichment and high resolution LC-MS/MS
Alzheimer’s disease (AD) is the most common form of dementia, characterized by progressive loss of cognitive function. One of the pathological hallmarks of AD is the formation of neurofibrillary tangles composed of abnormally hyperphosphorylated tau protein, but global deregulation of protein phosph...
ORGANISM(S): Homo sapiens (Human) 
2015-01-28 | PXD001180 | Pride
Phosphorylation Human brain Alzheimer's disease Phosphopeptide enrichment Lc-ms/ms 
ProteomicsAn Internal Standard for Assessing Phosphopeptide Recovery from Metal Ion/Oxide Enrichment Strategies
Phosphorylation-mediated signaling pathways have major implications in cellular regulation and disease. Mass spectrometry-based proteomics is a well-established approach for quantifying thousands of phosphorylation events in a single experiment. However, proteins with roles in signaling pathways are...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-12-04 | PXD008966 | Pride
Tmt super-heavy Lumos Phosphorylation Mtraq Tmtzero Tmtsh Tmt0 
ProteomicsAUTOMATED PHOSPHOPEPTIDE ENRICHMENT FROM MINUTE QUANTITIES OF FROZEN MALIGNANT MELANOMA TISSUE
To acquire a deeper understanding of malignant melanoma (MM), it is essential to study the proteome of patient tissues. In particular, phosphoproteomics of MM has become of significant importance because of the central role that phosphorylation plays in the development of MM. Investigating clinical ...
ORGANISM(S): Homo sapiens (Human) 
2018-11-23 | PXD011175 | Pride
Bravo assaymap Sensitivity Fe(iii)-imac. Malignant melanoma phosphoproteomics Phosphopeptide enrichment 
ProteomicsZirconium(IV)-IMAC for phosphopeptide enrichment in phosphoproteomics
IMAC and MOAC magnetic microsphere materials were tested with ug amounts of protein extracts from HepG2/C3A cells. We compared Zirconium (IV) IMAC (Zr-IMAC) magnetic microspheres to commonly used Titanium (IV) IMAC (Ti-IMAC) and TiO2 magnetic microspheres for phosphopeptide enrichment from simple an...
ORGANISM(S): Homo sapiens (Human) 
2020-11-26 | PXD018273 | Pride
Affinity enrichment Moac Imac Mass spectrometry Protein phosphorylation Functional proteomics Cell signalling Magnetic particles 
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