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ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030797 | Pride
Speed Plasma Kidney Sheep Tripletof S-trap Dda 
ProteomicsComparison of SPEED, S-Trap, and In-Solution based sample preparation methods for tandem mass spectrometry
Bottom-up proteomic analyses rely on efficient protein extraction from tissue and proteolysis into peptides for mass spectrometry. Commonly used detergent-based strategies aid cell lysis and protein solubilization but are poorly compatible with downstream protein digestion and liquid chromatography-...
ORGANISM(S): Ovis aries 
2023-03-30 | PXD030796 | Pride
Speed Plasma Kidney Sheep Swath-ms Tripletof S-trap 
ProteomicsSuspension trapping (S-trap) is compatible with typical protein extraction buffers and detergents for bottom up proteomics
Analysis of cells and tissue by bottom up proteomics starts with lysis, followed by in-solution digestion. Lysis buffers commonly used include detergents and other reagents for efficient solubility of the proteins. However these reagents are, for the most part, incompatible with downstream analytica...
ORGANISM(S): Homo sapiens (Human) 
2019-02-28 | PXD011665 | Pride
Q eaxctive hf Lysis Proteomics Hela S-trap 
ProteomicsS-Trap eliminates Pluronic F68 and cell culture media polymers for effective proteomic analysis of mammalian cell bioreactor supernatants
Proteomic analysis of bioreactor supernatants can inform on cellular metabolic status, viability, and productivity, as well as product quality, which can in turn help optimize bioreactor operation. Incubating mammalian cells in bioreactors requires the addition of polymeric surfactants such as Pluro...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2020-06-30 | PXD017214 | Pride
Polymer contaminants Alkylation Bottom-up proteomics Mass spectrometry Sp3 Sample preparation S-trap Bioreactor samples 
ProteomicsA sensitive S-trap-based approach to the analysis of T-cell raft proteome
The development of sensitive technologies for T cell-raft proteomics is highly challenging. We explored an innovative strategy, based on suspension trapping (S-trap) sample preparation. Mouse splenocytes were subjected to negative immunoselection for T cell preparation. Rafts were isolated by a dete...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2020-08-13 | PXD016476 | Pride
T cell Optiprep Raftomics Fasp S-trap Detergent-free 
ProteomicsS-Trap is an ultrafast sample preparation approach for shotgun proteomics
The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches such as gel-, solution- or filter-based, although being extensively employed in the field, are time-consuming and less effective with respect to the repetitive sample processing, recovery, and o...
ORGANISM(S): Escherichia coli 
2019-03-28 | PXD010237 | Pride
Proteomics  sample preparation  fasp  suspension trapping  klebsiella pneumoniae  sputum  tuberculosis. 
ProteomicsAn Optimized Suspension Trapping (S-Trap) Method for Phosphoproteomics Sample Preparation
The performance of the S-Trap protocol for phosphoproteomics studies for proteome and phosphoproteome was systematically evaluated in big-scale and small-scale samples. The results showed the existing protocol was problematic for phsophopeptides enrichment. After employing optimized S-Trap methodol...
ORGANISM(S): Homo sapiens (Human) 
2023-06-19 | PXD037751 | Pride
Phosphoproteomics  sample preparation  method optimization 
ProteomicsA Suspension Trapping-based Sample Preparation Workflow for Sensitive Plant Phosphoproteomics
Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
2023-08-28 | PXD039933 | JPOST Repository
TranscriptomicsA Safety and Efficacy Study of Concomitant Administration of ChAd63/MVA ME-TRAP + RTS,S
This clinical trial (NCT02252640) assesses the safety and immune responses to vaccination with experimental malaria vaccine candidates RTS,S/AS01, ChAd63 ME-TRAP and MVA ME-TRAP in different combinations. All healthy volunteers were recruited in England and administered the experimental malaria vacc...
ORGANISM(S): Homo sapiens 
2020-09-01 | GSE103874 | GEO
UnknownUnderstanding the mammalian TRAP complex function(s).
Not available
S-EPMC7276530 | biostudies-literature
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