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ProteomicsPandanus amaryllifolius drought stress and recovery LC-MSMS TMT-Labelled
The aim of this project is to determine the protein changes of P. amaryllifolius in response to drought stress and during recovery. The protein changes between drought-stressed, well-watered, and recovered plants were evaluated using tandem mass tags (TMT)-based quantitative proteomics. The proteins...
ORGANISM(S): Pandanus amaryllifolius 
2022-02-16 | PXD028784 | Pride
Lc-msms Drought stress Abiotic stress Pandanus amaryllifolius Tmt-labelled Recovery 
ProteomicsK - TMT-6 plex labelled plasma ratio's 1:1:3:3:9:9
TMT-6 plex labelled plasma ratio's 1:1:3:3:9:9
ORGANISM(S): Homo sapiens (Human) 
2010-05-26 | PRD000247 | Pride
ProteomicsOptimising HCD fragmentation energy for TMT-labelled intact glycopeptide samples
It was crucial to choose the appropriate dissociation method and collision energy for large-scale glycoproteomics studies. HCD has been successfully applied to large-scale glycoproteomics experiments31. We know that TMT-labelled glycopeptides require higher collision energies for identification and ...
ORGANISM(S): Mus Musculus 
2025-01-20 | PXD060000 |
ProteomicsHuman vitreous TMT labelled LC-MS/MS
Proliferative diabetic retinopathy (PDR) is a severe complication of diabetes and can cause blindness. However, the available therapeutic modalities to PDR have unsatisfactory efficacies and incur adverse effects, which is due to the paucity in the understanding of pathogenic mechanisms responsible ...
ORGANISM(S): Homo sapiens (Human) 
2020-12-18 | PXD021788 | Pride
Ctsd Proteomics Ctsb Proliferative diabetic retinopathy Lc-ms/ms 
ProteomicsHuman Yeast Dilution TMT labelled Rescored with Prosit.
We Have a human yeast dilution dataset in this project. We predict all spectra in the datasets via Prosit then rescore. We have 100% FDR max quant search results using percolator. In addition, we get 1%FDR filtered results with andromeda Scores and another with features extracted from Prosit predict...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2022-06-09 | PXD030340 | Pride
Human Cid Ot Yeast Hcd Tmt11plex It Orbitrap Iontrap 
ProteomicsAbsolute Quantification of Yeast Proteins Using Synthetic Peptide Standards and TMT labeling
The absolute protein amounts for the selected yeast ribosomal proteins (RPs) were determined using the standard peptides and TMT labelling. The range of the standard tryptic peptides were selected and synthesized based on the preliminary data-dependent experiments. The synthetic peptides were mixed ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-13 | PXD015025 | Pride
Tmt Yeast Tandem mass tag Standard peptides Saccharomyces cerevisiae Absolute quantification Synthetic peptides Orbitrap fusion 
ProteomicsCDKL5 substrates - Phosphoproteomic screening identifies physiological substrates of the CDKL5 kinase
Identification of CDKL5 substrates. Immuno-precipitated proteins were TMT labelled and analysed via LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2018-10-02 | PXD009327 | Pride
Cdkl5 Tmt Dlg5 map1s Cep131 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches which rely on complete labelling of primary amines. However, serines, tyrosi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2025-03-14 | PXD056723 | Pride
Phospho-peptides Tmt N-hydroxy succinimide Methylamine Nhs-ester Hydroxylamine 
ProteomicsYTHDF1 links hypoxia adaptation and non-small cell lung cancer progression
10plex TMT labelling quantification expriments were performed to access the knockdown effect of YTHDF1 in cells.3 shGFPs control samples, 3 YTHDF1 shRNA-2 knockdown samples , 3 YTHDF1 shRNA-3 knockdown samples and 1 mixture sample were labelled by TMT 10-plex reagents.
ORGANISM(S): Homo sapiens (Human) 
2019-09-27 | PXD015182 | Pride
Lc-ms/ms tmt10plex 
ProteomicsRemoval of NHS-labelling by-products in proteomic samples
N-Hydroxysuccinimide (NHS) chemistry is used extensively across proteomics sample preparation. One of the most popular applications is its use in isobaric reagent based quantitation; specifically both the iTRAQ and TMT approaches. The labels are introduced onto primary Serines, tyrosines, and threon...
ORGANISM(S): Homo sapiens (Human) 
2025-03-14 | PXD054730 | Pride
Nhs Methylamine Hydroxylamine N-hydroxysuccinimide O-acylation 
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