Sort   by:  
 Page size 
Raw mass spectrometry data (.d raw data from Bruker) supporting the study: "A conserved ER protein prevents lipotoxicity by stimulating the key enzyme in glycerolipid synthesis". This dataset contains LC-MS/MS raw files from a proximity labeling experiment (TurboID) designed to identify interacting ...
ORGANISM(S): Homo sapiens (Human) 
2026-04-09 | PXD075982 | Pride
Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. Using the highly active biotin ligase TurboID, we optimize a proximity labeling protocol for C. elegans. We use this protocol to characterise the prote...
ORGANISM(S): Caenorhabditis elegans 
2021-09-15 | PXD027068 | Pride
Proximity labeling (PL) was recently developed to detect protein–protein interactions (PPIs) and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (ba...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-26 | PXD043041 | Pride
To review an improved proximity-labeling strategy that uses the improved E. coli biotin ligase TurboID to characterize C. elegans protein complexes, the interactome of a presynaptic active zone protein, ELKS-1, was analyzed as proof of principle. A significant constraint on the sensitivity of TurboI...
ORGANISM(S): Caenorhabditis elegans 
2022-09-12 | PXD034639 | Pride
TurboID-mediated proximity labeling of the aging and stress regulatory transcription factor DAF-16/FOXO in wild-type and daf-2(e1370) mutants in C. elegans. Endogenous DAF-16 was C-terminally tagged with the biotin ligase TurboID using CRISPR/Cas9- genome editing. In vivo proximity labeling of the ...
ORGANISM(S): Caenorhabditis elegans 
2025-12-29 | PXD053591 | Pride
Identifying specific protein interactors and spatially or temporally restricted local proteomes contributes significantly to our understanding of cellular processes in virtually all aspects of life. Obtaining such data is challenging, especially when the protein, cell type or event of interest is ra...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-25 | PXD015161 | Pride
Oral squamous cell carcinoma (OSCC) is a prevalent type of head and neck cancer, accounting for more than 90% of all oral malignancies worldwide. The identification of diagnostic and prognostic markers for OSCC is crucial for improving patient outcomes, as early detection and treatment are critical ...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD048046 | Pride
Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which ...
ORGANISM(S): Toxoplasma gondii GT1 
2024-07-12 | PXD039986 | Pride
Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which ...
ORGANISM(S): Toxoplasma gondii GT1 
2024-07-12 | PXD039985 | Pride
Sort   by:  
 Page size