Sort   by:  
 Page size 
MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsPKC isoforms activate LRRK1 kinase by phosphorylating conserved residues (Ser1064, Ser1074 and Thr1075) within the CORB GTPase domain
Leucine-rich-repeat-kinase 1 (LRRK1) and its homologue LRRK2 are multidomain kinases possessing an atypical ROC-CORA-CORB containing GTPase domain and phosphorylate distinct Rab proteins. LRRK1 loss of function mutations cause the bone disorder osteosclerotic metaphyseal dysplasia, whereas LRRK2 mis...
ORGANISM(S): Homo sapiens (Human) 
2022-09-02 | PXD034420 | Pride
Rab7a Lrrk1 Pkc Phosphorylation 
ProteomicsGolgi-IP, a novel tool for multimodal analysis of Golgi molecular content
The Golgi plays an essential role in protein and lipid glycosylation. Golgi dysfunction is associated with a variety of diseases including cancer, neurodegeneration and cardiovascular disease. We developed a powerful immunoprecipitation technique to isolate the Golgi by tagging TMEM115. Golgi-immuno...
ORGANISM(S): Homo sapiens (Human) 
2023-04-24 | PXD038046 | Pride
Golgi-ip Proteomics Metabolomics Lipidomics Golgi apparatus 
ProteomicsStructural basis for the specificity of PPM1H phosphatase for Rab GTPases
LRRK2 serine/threonine kinase is associated with inherited Parkinson’s disease. LRRK2 phosphorylates a subset of Rab GTPases within their switch 2 motif to control their interactions with effectors. Recent work has shown that the metal-dependent protein phosphatase PPM1H counteracts LRRK2 by dephosp...
ORGANISM(S): Homo sapiens (Human) 
2021-08-11 | PXD026367 | Pride
Rab8a Ppm1h Dsbu crosslinking 
ProteomicsDevelopment of a multiplex targeted mass spectrometry assay to quantify levels of LRRK2 phosphorylated Rab substrates and Ser910 and Ser935 biomarker sites
Mutations that increase the protein kinase activity of LRRK2 are one of the most common causes of familial Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases within their Switch-II motif, impacting interaction with effectors. We describe and validate a new, multiplex targeted mass spe...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-03-17 | PXD022662 | Pride
Rab-gtpases Parkinson's disease Lrrk2 Prm Lc-ms/ms 
ProteomicsParkinson’s VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B
The D620N VPS35 mutation disrupts retrograde endosome to Golgi retromer Trafficking. Thus causes lysosome dysfunction, enhances LRRK2 kinase activity and leads to Parkinson’s disease. We employed a LysoTag immunoprecipitation approach to assess how this impacts lysosomes using quantitative proteomic...
ORGANISM(S): Mus musculus (Mouse) 
2023-11-28 | PXD042502 | Pride
Parinson's disease  lysosomal proteins  lrrk2  rilpl1  tmem55b 
ProteomicsDiscovery of XL01126: A Potent, Fast, Cooperative, Selective, Oral bioavailable and Blood Brain Barrier Penetrant PROTAC Degrader of Leucine Rich Repeat Kinase 2 (LRRK2)
Leucine Rich Repeat Kinase 2 (LRRK2) is one of the most promising targets for Parkinson’s Disease. LRRK2 targeting strategies have primarily focused on Type 1 kinase inhibitors, which however have limitations as the inhibited protein can interfere with natural mechanisms and lead to undesirable side...
ORGANISM(S): Mus musculus (Mouse) 
2022-09-02 | PXD034055 | Pride
Parkinsosn's disease Tmt Lrrk2 Mass spectrometry Protac 
ProteomicsStructural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif
Inherited mutations in VPS35 and the kinase LRRK2 lead to hyperphosphorylation of Rab GTPases and promote the formation of phospho-Rab signalling complexes. A subset of RH2 domain-containing proteins from the RILP-homology family, including RILP, RILPL1, RILPL2, JIP3, and JIP4 are Rab effectors that...
ORGANISM(S): Homo sapiens (Human) 
2025-11-30 | PXD067414 | Pride
Lysosome Lrrk2 Tmem55b Parkinson's disease Structure 
ProteomicsThe Parkinson’s disease kinase LRRK2 impairs release sites of vulnerable dopamine axons
Data relating to the section: "Phosphorylation of Rab3a decreases its binding to the effector proteins Rim1&2 from mouse brain". The data of this submission is part of a larger screen of Rab/pRab interactors, and therefore, DIA-NN search output files contain additional sample columns that remain bl...
ORGANISM(S): Mus musculus (Mouse) 
2026-05-27 | PXD065125 | Pride
Rab3a Lrrk2 Dopamine neurons Phosphorylation 
ProteomicsNRBP1 pseudokinase binds to and activates the WNK pathway in response to osmotic stress - Figure 1D/S1C
Please refer to the manuscript for a detailed description.
ORGANISM(S): Homo sapiens (Human) 
2025-07-21 | PXD062616 | Pride
Osmotic stress Wnk pathway Nrbp1 pseudokinase 
Sort   by:  
 Page size