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UnknownDissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP).
Not available
S-EPMC6101743 | biostudies-literature
ProteomicsFeed-forward stimulation of CAMK2 by the oncogenic pseudokinase PEAK1 generates an ‘actionable’ signalling axis in triple negative breast cancer
To characterize the interactomes of PEAK1 and PEAK2 homodimers, and the PEAK1/PEAK2 heterodimer, we applied bimolecular complementation affinity purification coupled with tandem mass spectrometry (BiCAP-MS/MS), a recently-developed technique that specifically identifies the interactors of any pair o...
ORGANISM(S): Homo sapiens (Human) 
2025-01-04 | PXD044872 | Pride
Ms Dimerization Bicap Hek293t Interactome 
ProteomicsER-phagy regulates ER dynamics during myogenesis (5)
IP-MS interactome of FAM134B1 and FAM134B2 complexes (bimolecular complementation affinity purification (BiCAP) system)
ORGANISM(S): Mus musculus (Mouse) 
2024-12-27 | PXD050902 | Pride
Muscle Autophagy Endoplasmic reticulum Erphagy Fam134b 
ProteomicsTECPR2 knock-in mice proteomics
Mutation in the gene encoding Tectonic -propeller repeat-containing repeat protein 2 (TECPR2) leads to hereditary sensory and autonomic neuropathy type 9 (HSAN9) which is a fatal complex neurodevelopmental and neurodegenerative disorder involving the sensory and peripheral nervous system. TECPR2 is ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-11-10 | PXD059099 | Pride
Bicap Endoip Hsan9 Microglia Tecpr2 
ProteomicsIRGQ-GABARAPL2 complex interactome
GABARAPL2 is the only hATG8 able to fit in the LIR1-G1 loop pocket of IRGQ. To further dissect this striking evolutionary specificity, we queried the interactome of the IRGQ-GABARAPL2 complex with proteomics by using the Venus complementation affinity purification (BiCAP) method.
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD066671 | Pride
Irgq Autophagy Gabarapl2 
ProteomicsPSKH1 kinase activity is differentially modulated via allosteric binding of Ca2+ sensor proteins
Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed in prostate, lung and kidney cancers. However, little is known about PSKH1’s regulation, leading to its classification as a “dark” kinase. Here, we used biochemistry and mass spect...
ORGANISM(S): Homo sapiens (Human) 
2025-01-26 | PXD056543 | Pride
Protein kinase  allosteric regulation  calumenin  erc-55  cab-45 
ProteomicsPSKH1 kinase activity is differentially modulated via allosteric binding of Ca2+ sensor proteins
Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed in prostate, lung and kidney cancers. However, little is known about PSKH1’s regulation, leading to its classification as a “dark” kinase. Here, we used biochemistry and mass spect...
ORGANISM(S): Homo sapiens (Human) 
2025-01-26 | PXD058954 | Pride
Calcium Ionomycin Pskh1 
ProteomicsDynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cells
Integrins provide an essential bridge between cancer cells and the extracellular matrix, playing a central role in every stage of disease progression. Despite the recognized significance of integrin phosphorylation in several biological processes, the regulatory mechanisms and their relevance remain...
ORGANISM(S): Homo sapiens (Human) 
2025-02-05 | PXD048646 | Pride
Breast cancer Phosphorylation Integrins Extracellular matrix 
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