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ProteomicsProximity biotinylation and LC-MSMS identification of the molecular microenvironment of coronavirus replication complexes
To investigate virus-host interactions at the site of coronavirus replication, we developed a biotin-based proximity labelling approach by engineering a promiscuous biotin ligase, BirA-R118G, within the coronavirus replication and transcription complex (RTC). BirA-R118G was fused to non-structural p...
ORGANISM(S): Murine hepatitis virus strain A59 Mus musculus (Mouse) 
2019-01-16 | PXD009975 | Pride
L929 mouse fibroblast Lc-msms Viral replication complex Bira-r118g Mouse hepatitis virus Bioid 
ProteomicsPABPC1 stress granule interactome identified by BioID
Identification of Stress Granule interacting proteins by BioID (Roux et al., 2012) using PABPC1-BirA(R118G)-GFP construct
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD025334 | Pride
Stress granule Human Pabpc1 
UnknownA Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types.
Not available
S-EPMC6210231 | biostudies-literature
UnknownIdentification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).
Not available
S-EPMC4774922 | biostudies-literature
ProteomicsBoDV-1 phosphoprotein BioID in HEK293
Here we have identified human proteins in spatial proximity (proximal interaction network) of BoDV-1 Phosphoprotein fused to BirA* (E.Coli biotin ligase R118G abortive mutant) at the N-ter and C-ter ends in human cells by proximity-dependent biotinylation (BioID)-based approach. We report 301 high-c...
ORGANISM(S): Homo sapiens (Human) 
2026-01-27 | PXD036750 | Pride
Bioid Bodv-1 Human Phosphoprotein Proximal interactomics 
ProteomicsThe co-chaperone DNAJA2 buffers proteasomal degradation of cytosolic proteins with missense mutations
Submission contains data from proximity labeling combined to mass spectrometry (BioID) with protein of interest TPMT fused in-frame with BirA-R118G carboxylase expressed in HEK293 T-REx Flp-In cells. Proximity interacting proteins were captured with streptavidin sepharose, digested with trypsin and ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-10 | MSV000094061 | MassIVE
ProteomicsProximity proteomics provides a new resource for exploring the function of Afadin and the complexity of cell-cell adherens junctions
Afadin and its Drosophila homolog Canoe are key components of the network of proteins that help link cell-cell adherens junctions to the actomyosin cytoskeleton. They stabilize this connection as force is generated to drive cell shape change and migration during embryonic morphogenesis, organogenes...
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2025-01-20 | PXD056971 | Pride
Bioid Afadin Cell-cell adherens junctions 
ProteomicsIdentification of Stress Granule interactor proteins in yeast S. cerevisiae
We investigated the sub-cellular architecture of Stress Granules in the context of glucose starvation. In order to uncover transient and peripheral Stress Granule interactors, we optimized the proximity-dependent biotinylation (BioID) approach for use in yeast. This approach allowed us to visualize ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-23 | PXD016988 | Pride
Stress granule Pab1 Pub1 Bioid 
ProteomicsCD95/Fas suppresses NF-B activation through recruitment of KPC2 in a CD95L/FasL-independent mechanism
CD95 expression is preserved in triple-negative breast cancers (TNBCs) and CD95 loss in these cells triggers the induction of a pro-inflammatory program promoting the recruitment of cytotoxic NK cells impairing tumor growth. Herein, we identify a novel interaction partner of CD95, Kip1 ubiquitinatio...
ORGANISM(S): Homo sapiens (Human) 
2021-12-06 | PXD027196 | Pride
Kpc2 Inflammation Nf-kappab Fas P65 
ProteomicsIdentification of proximal SUMO-dependent interactors using SUMO-ID
Proximity-dependent labelling (PL) methods are based on promiscuous labeling enzymes that produce reactive molecules that covalently bind neighbor proteins. Labeled proteins can be then purified and identified using affinity-purification coupled to mass spectrometry methods39. Proximity-dependent bi...
ORGANISM(S): Homo sapiens (Human) 
2021-09-20 | PXD021809 | Pride
Pml Proximity-dependent labelling Sumo Turboid Protein interaction 
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