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ProteomicsNALCN crosslinking experiments by BS3 crosslinker
We used BS3 as the crosslinker to identify the crosslinking sites between NALCN and other interacted proteins.
ORGANISM(S): Homo sapiens (Human) 
2020-12-04 | PXD022119 | Pride
Nalcn Crosslinking Lc-ms/ms 
ProteomicsBS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)
How Apc8-LOOP affects the displacement of Apc1-300LOOP was investigated using crosslinking mass spectrometry (MS) to gain insight into the point of interaction between disordered Apc8-LOOP and the APC/C center structure.
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-09-08 | PXD044827 | Pride
Crosslinking mass spectrometry Disordered loop domains Phosphorylation Apc/c 
ProteomicsCrosslinking MS to elucidate the Architecture of the Nucleosome Remodelling and Deacetylase (NuRD) complex
The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for development in complex animals but has been refractory to detailed analysis because of its low abundance and resistance to recombinant production. In combination with other techniques, crosslinking mass spectrometry was used t...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2021-01-08 | PXD010111 | Pride
Crosslinking ms Structural proteomics Nurd 
ProteomicsBS3 crosslinking of GRC3 and Las1
Ribosome assembly is a complex process involving the coordination of multiple enzymes. A recently discovered endoribonuclease (RNase), Las1, and the poly-nucleotide kinase (PNK), Grc3, assemble into a complex. To attempt to understand the coordination of the two enzymes in this complex, we performed...
ORGANISM(S): Chaetomium thermophilum 
2019-09-11 | PXD014738 | Pride
Crosslink grc3 las1 bs3 
ProteomicsBS3 crosslinking of the human TSEN complex
BS3 crosslinking of the human TSEN complex The tRNA splicing endonuclease (TSEN) complex performs the first step in the maturation of the anticodon stem loop of tRNAs. To date, eukaryotic TSEN complexes have been refractory to structural elucidation and this lack of structural information has hinde...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-01-25 | MSV000091153 | MassIVE
ProteomicsBS3 crosslinking of the Anaphase-Promoting Complex/Cyclosome (APC/C)
In order to investigate the dynamic regulation of the APC/C by intrinsically disordered regions (IDRs) upon phosphorylation, we employed cross-linking mass spectrometry (CLMS). Given that the phosphorylation of Apc1-300L (IDR in Apc1) is a key determinant in its release from the co-activator binding...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-04-23 | PXD050097 | Pride
Crosslinking mass spectrometry The anaphase-promoting complex/cyclosome (apc/c) 
ProteomicsCXMS BS3 Ras/BRAF/MEK/1433 complex
RAF-family kinases are activated by recruitment to the plasma membrane by GTP-bound RAS, where they initiate signaling through the MAP kinase cascade. Here we crosslinked (BS3), digested (trypsin), and analyzed via LC-MS/MS KRAS (human, residues 1-169) bound to intact BRAF (human) in an autoinhibit...
ORGANISM(S): Spodoptera frugiperda Homo sapiens (Human) 
2023-10-24 | PXD042584 | Pride
Cxms Human Braf Mek1 14-3-3 Sf9 Kras 
ProteomicsThe ubiquitin-like modifier FAT10 interferes with SUMO activation
In this study, the proteins SumoE1 and Fat10 or Fat10-AV mutant were crosslinked using the crosslinking reagent H12/D12 BS3 in order to determine specific interaction sites of Fat10 and SumoE1. Furthermore, quantitative chemical crosslinking coupled to mass spectrometry (q-XL-MS) was used to compare...
ORGANISM(S): Homo sapiens (Human) 
2019-10-08 | PXD012592 | Pride
Fat10 Q-xl-ms Xl-ms Sumoe1 
UnknownBis(sulfosuccinimidyl) suberate (BS3) crosslinking analysis of the behavior of amyloid-β peptide in solution and in phospholipid membranes.
Not available
S-EPMC5360245 | biostudies-literature
ProteomicsTo decipher an overall structure of a bacterial anti-plasmid defense system using cross-linking mass spectrometry.
The submitted data is from a BS3 crosslinked sample. The anti-plasmid defense system complex constitutes JetA, JetB, JetC, and JetD subunits. In this work, these four subunits were mixed to form a holo-subunit complex which was followed by crosslinking with isotopically/non-isotopically (1:1) mixtur...
ORGANISM(S): Pseudomonas aeruginosa PA14 
2022-10-06 | PXD031096 | Pride
Bacterial anti-plasmid defense system Cross-linking mass spectrometry Jetabcd Condensin like machinery Toprim 
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