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ProteomicsMetalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Removal
Cytotoxicity of DNA-protein crosslinks (DPCs) is ascribed largely to their ability to block the progression of DNA replication fork. DPCs are frequently occurring in cells, either as a consequence of metabolism or exogenous agents. The mechanism of DPCs removal is not completely understood. Here, we...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080743 | MassIVE
ProteomicsUSP11 deubiquitinates monoubiquitinated SPRTN to repair DNA-protein crosslinks
DNA-protein crosslinks (DPCs) are toxic DNA lesions that interfere with DNA metabolic processes such as replication, transcription, and recombination. SPRTN is a replication-coupled DNA-dependent metalloprotease that degrades proteins crosslinked to DNA to promote DPC repair. SPRTN function is tight...
ORGANISM(S): Homo sapiens (Human) 
2021-04-01 | PXD019923 | Pride
Deubiquitination Dpc Top1-ccs Dpc repair Monoubiquitinated sprtn Sprtn Usp11 
ProteomicsDNA replication regulates SPRTN- and Proteasome-dependent DPC proteolysis
DNA-protein crosslinks (DPCs) are bulky DNA lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S-phase removal of DPCs, but how SPRTN activity is coupled to DNA replication is unknown. Using Xenopus egg e...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2019-02-13 | PXD008831 | Pride
Plasmid pull-down Pp-ms Dpc repair Dna repair Xenopus egg extract Dna replication 
ProteomicsPARP1-dependent DNA-protein crosslink repair
DNA-protein crosslinks (DPCs) are toxic lesions that inhibit DNA related processes. Post-translational modifications (PTMs), including SUMOylation and ubiquitylation, play a central role in DPC resolution, but whether other PTMs are also involved remains elusive. Here, we identify a DPC repair pathw...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2024-08-06 | PXD043107 | Pride
Xenopus Parp1 Top1 Dna-protein crosslink Adp-ribosylation Ddi2 Dna damage Ubiquitin Sumo P97 
GenomicsDNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
Covalent DNA-protein crosslinks (DPC) are toxic DNA lesions that require repair by global-genome and replication-coupled pathways. How cells respond when RNA polymerases stall at DPCs during transcription is unknown. DPC-seq is new method for the genome-wide mapping of covalent DNA-protein adducts.
ORGANISM(S): Homo sapiens 
2024-02-24 | E-MTAB-12912 | biostudies-arrayexpress
UnknownRepair pathway for PARP-1 DNA-protein crosslinks.
Not available
S-EPMC6312470 | biostudies-literature
GenomicsDNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
DNA-Protein Crosslink sequencing (DPC-seq) in RPE-1 cells treated with formaldehyde
PRJEB73285 | ENA
UnknownSPRTN-dependent DPC degradation precedes repair of damaged DNA: a proof of concept revealed by the STAR assay.
Not available
S-EPMC10085693 | biostudies-literature
ProteomicsMechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO pathway
DNA-protein crosslinks (DPCs) obstruct essential DNA transactions, posing a serious threat to genome stability and functionality. DPCs are proteolytically processed in a ubiquitin- and DNA replication-dependent manner by SPRTN or the proteasome but can also be resolved via targeted SUMOylation. Howe...
ORGANISM(S): Xenopus laevis (African clawed frog) 
2021-08-06 | PXD021947 | Pride
Rnf4 Xenopus Dpc Chromass Dna damage Replication Sumo Pias4 
UnknownOxidative DNA-protein crosslinks formed in mammalian cells by abasic site lyases involved in DNA repair.
Not available
S-EPMC7065521 | biostudies-literature
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