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ProteomicsTemporal and thermal optimization of trypsin digestion for the cryptococcal proteome
Mass spectrometry-based proteomics supports investigation into diverse biological systems, providing insights into regulation, signaling, modifications, and interactions. Optimized sample preparation is critical to ensure robust and reproducible analyses balanced with time and cost. In this article,...
ORGANISM(S): Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) 
2024-10-12 | PXD056755 | Pride
Cryptococcus neoformans Total proteome Trypsin 
ProteomicsPrenylcysteine oxidase 1 like protein is required for neutrophil bactericidal activities
The bactericidal function of neutrophils are dependent on myriad intrinsic and extrinsic stimuli. Using systems immunology approaches we identified microbiome- and infection-induced changes in neutrophils. We focused on investigating the Prenylcysteine oxidase 1 like (Pcyox1l) protein function. Muri...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-14 | PXD031115 | Pride
Neutrophil Prenylation Pcyox1l 
ProteomicsFungal-bacterial co-infection of macrophages reveals global reprogramming and adaptation during infection
In this study, we use mass spectrometry (MS)-based proteomics to define the host response under single and dual infection states of macrophages with C. neoformans and K. pneumoniae to define critical responses of each biological system. This high-resolution comparative analysis illustrates how prote...
ORGANISM(S): Mus musculus (Mouse) Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) Klebsiella pneumoniae 
2022-06-30 | PXD033393 | Pride
Quantitative proteomics Macrophages Pathogenesis Cryptococcus neoformans Co-infection Klebsiella pneumoniae 
ProteomicsQuantitative proteomics of iron limitation in Klebsiella pneumoniae
Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, iron is an essential component for many cellular processes and bioavailability varies greatly among different conditions. In the bacterium, Klebsiella pneumoniae, the impact ...
ORGANISM(S): Klebsiella pneumoniae 
2020-03-09 | PXD015623 | Pride
Quantitative proteomics Lon protease Iron limitation Klebsiella pneumoniae Secretome 
ProteomicsZinc limitation in Klebsiella pnuemoniae
Nutrient adaptation is key in limiting environments for the promotion of microbial growth and survival. In microbial systems, zinc is an important component for many cellular processes and bioavailability varies greatly among different conditions. In the bacterium, Klebsiella pneumoniae, the impact ...
ORGANISM(S): Klebsiella pneumoniae 
2021-02-12 | PXD016042 | Pride
Virulence Klebsiella Transport Quantiative proteomics Zinc Proteome Secretome 
ProteomicsNanochip proteome of Klebsiella
In this study, we used an interdisciplinary approach, combining biophysics and systems biology, to design a microfluidic chip for the multiplexed detection and separation of pathogen cells from immune cells combined with proteomic profiling using mass spectrometry. Our nanochip design includes preci...
ORGANISM(S): Klebsiella pneumoniae subsp. pneumoniae 
2026-03-21 | PXD058426 | Pride
Quantitative proteomics Microfluidics Klebsiella pneumoniae Nanochip technology Macrophages Anti-virulence Systems biology 
ProteomicsAre there enzymatic treatment options for ocular biofilm-based infections?
Pseudomonas aeruginosa-induced corneal keratitis is a sight-threatening disease. The elevated rise of antibiotic resistance among the Pseudomonas keratitis isolates makes treatment of this disease challenging, justifying the need for alternative therapeutic modalities or treatment strategies that ca...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-15 | PXD009767 | Pride
Keratitis P. aeruginosa Neutrophils Enzymatic treatment Biofilms 
ProteomicsInhibition of ß1 integrin induces its association with MT1-MMP and decreases MT1- MMP internalization and cellular invasiveness
Integrin signaling plays a fundamental role in the establishment of focal adhesions and the subsequent formation of invadopodia in malignant cancer cells. Invadopodia facilitate localized adhesion and degradation of the extracellular matrix (ECM), which promote tumour cell invasion and metastasis. D...
ORGANISM(S): Homo sapiens (Human) 
2022-12-22 | PXD024000 | Pride
Extracellular matrix  integrin  mt1-mmp  invadopodia 
ProteomicsThe ER protein translocation channel subunit Sbh1 controls virulence of Cryptococcus neoformans
C. neoformans is an opportunistic human pathogen whose polysaccharide capsule anchored to the cell wall is critical for virulence. Biogenesis of both cell wall and capsule relies on the secretory pathway. Protein secretion begins with protein translocation across the endoplasmic reticulum (ER) membr...
ORGANISM(S): Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) 
2023-01-27 | PXD013894 | Pride
Secretion Proteomics Cryptococcus neoformans Er 
ProteomicsTemporal proteomic profiling of Agrobacterium tumefaciens exposure to agroinfiltration media influences cellular remodeling and may promote enhanced adaptability for molecular farming
Plants transformed via agroinfiltration using a modified strain of Agrobacterium tumefaciens can produce antibody proteins for purification and application in antibody-based therapeutics. The preparation of large quantities of plants for transformation may require bacterial exposure to agroinfiltrat...
ORGANISM(S): Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter) 
2020-12-10 | PXD018405 | Pride
Trastuzumab Bioreactor Agrobacterium tumefaciens Agroinfiltration media Shake flask Quantitative proteomics 
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