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ProteomicsSGBS iron secretome - To identify differentially expressed proteins in the secretome of differentiated SGBS cells human adipocytes) in response to iron.
To identify differentially expressed proteins in the secretome of differentiated SGBS cells human adipocytes) in response to iron.
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD006341 | Pride
Sgbs Adipocytes Iron Secretome 
ProteomicsOvarian Cancer Lipid raft proteomics and role of OPCML
ipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions and are implicated in cancer development. To understand potential role of tumor suppressor OPCML on the altered dynamics of lipid raft residing proteins, we performed quantitative (SILAC) proteomics experiment. We ...
ORGANISM(S): Homo sapiens (Human) 
2017-03-16 | PXD006078 | Pride
Skov-3 Silac P95r opcml Lipid raft Opcml 
ProteomicsAntibody-free multiplex measurement of 23 human cytokines in primary cell culture secretome using targeted multiple reaction monitoring mass spectrometry
Cytokines are commonly measured by immunoassays, which have limited multiplexing capacity, are costly and can exhibit cross-reactivity. Although multiple reaction monitoring (MRM) mass spectrometry is a cost-effective method for multiplex measurement of peptides, the ability to detect the relatively...
ORGANISM(S): Homo sapiens (Human) 
2020-02-19 | PXD015407 | Pride
Human Cytokine Secretome 
ProteomicsOptimising size exclusion chromatography for extracellular vesicle enrichment and proteomic analysis from clinically relevant samples
We performed a quantitative evaluation and optimization of SEC for separating EVs from contaminating proteins. This optimized method was applied to enrich EVs from healthy plasma and MDA-MB-231 cancer cell culture medium. By spiking-in cancer cell-derived EVs to healthy plasma and then performing SE...
ORGANISM(S): Homo sapiens (Human) 
2019-01-16 | PXD010341 | Pride
Plasma Chromatography Extracellular vesicle 
ProteomicsA primary human T-cell spectral library to facilitate large scale quantitative T-cell proteomics
Comprehensive spectral libraries are essential in sequential window acquisition of all theoretical mass spectra (SWATH-MS) based high throughput proteomic studies. Even though SWATH-MS assays provide robust quantitative proteomics data its applications to human T-cell studies are limited by the lack...
ORGANISM(S): Homo sapiens (Human) 
2020-10-19 | PXD019446 | Pride
Human Swath Spectral library Primary t-cells 
ProteomicsCD49f defines a subset of dysfunctional regulatory T cells circulating in the human peripheral blood
The therapeutic use of regulatory T cells (Tregs) in patients with autoimmune disorders has been hampered by the biological variability of memory Treg populations in the peripheral blood. In this study, we reveal through a combination of quantitative proteomic, multiparametric flow cytometry, RNA-se...
ORGANISM(S): Homo sapiens (Human) 
2021-08-06 | PXD022095 | Pride
Human Regulatory t cells Shotgun proteomics 
ProteomicsDiscovery and validation of serum glycoprotein biomarker panel for detection of high grade serous ovarian cancer
We aimed to discover and validate a panel of serum biomarkers for high grade serous ovarian cancer (HGSOC) using our lectin-magnetic bead array-coupled proteomics platform. Serum from age-matched women with HGSOC, benign tumours or healthy controls were analysed in discovery (UKCTOCS, n=30 and UKOPS...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD032299 | Pride
Ovarian cancer screening Stl Glycosylation Lemba Lectin 
ProteomicsSkin cancer-associated S. aureus downregulates DNA repair mechanisms, and promotes oxidative stress and DNA damage in human keratinocytes
Actinic keratoses (AK) are premalignant lesions common on photo-damaged skin that, over time, can progress to squamous cell carcinoma (SCC). A high bacterial load of Staphylococcus aureus is associated with AK and SCC but it is unknown whether this has a direct impact on skin cancer development. To ...
ORGANISM(S): Homo sapiens (Human) 
2022-05-11 | PXD031622 | Pride
S. aureus secretome Multiomics Actinic keratosis Skin Dna damage Squamous cell carcinoma 
ProteomicsDetection SARS-CoV-2 infection in mice by ATR-FTIR
Vero-E6 cell supernatant, mice lavage and human saliva were evaulated after exposure to SARS-CoV-2 via ATR-FTIR spectoscopy. To provide further information about the physiological response, proteins contained in the mouse lavage were assessed via shotgun proteomics (data shown here). Mice were expos...
ORGANISM(S): Homo sapiens (Human) 
2021-12-17 | PXD030012 | Pride
Sars-cov-2 Ftir Covid-19 
ProteomicsIdentification of candidate circulating glucocorticoid response biomarkers in humans using a proteomic approach
Glucocorticoids used in pharmacological doses for the treatment of a variety of medical conditions, and endogenous glucocorticoid excess – Cushing’s syndrome, may result in several adverse effects, but currently there is no clinically useful biomarker of glucocorticoid activity. A three-phase prote...
ORGANISM(S): Homo sapiens (Human) 
2021-03-14 | PXD019945 | Pride
Pbmc Glucocorticoid Proteomics Dexamethasone Biomarker 
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