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ProteomicsGeneration of a C3a-specific monoclonal antibody and immunoapheresis of C3a/C3 and its associates
A high affine murine monoclonal antibody was generated against complement factor C3 and selected for binding to the C3a region to serve as immunoaffinity reagent. Subsequently, a C3a/C3 specific immunoaffinity column was developed and apheresis of C3a/C3 and its associates was performed. A proteomic...
ORGANISM(S): Homo sapiens (Human) 
2019-11-27 | PXD009829 | Pride
Electrophoresis Monoclonal antibody Mass spectrometry Immunoapheresis Complement factor c3a Proteomic analysis. 
ProteomicsPotato ATG8 interactome - N-terminal β-strand underpins biochemical specialization of an ATG8 isoform
GFP-IP based interactome of potato ATG8 isoforms following transient expression in Nicotiana benthamiana.
ORGANISM(S): Nicotiana benthamiana 
2019-11-12 | PXD011226 | Pride
Autophagy Atg8 Plant Nicotiana benthamiana 
ProteomicsAffinity proteomics targeting the meiotic chromosome axis protein, ASY1, from Brassica oleracea.
Meiosis is a highly complex process that underpins recombination in sexually reproducing organisms. Recent genomics studies suggest that up to several thousand genes/proteins contribute to the meiotic pathway, yet relatively few have been functionally characterised. Our understanding of the physical...
ORGANISM(S): Brassica oleracea var. alboglabra 
2017-11-01 | PXD006042 | Pride
Arabidopsis Chromosome axis Brassica Co-immunoprecipitation Protein-protein interaction Phosphorylation Meiosis Lc-ms/ms 
ProteomicsBRD4 IP MS in leukemia - MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation
The histone acetyl-reader BRD4 is an important regulator of chromatin structure and transcription, yet factors modulating its activity have remained elusive. Here we describe two complementary screens for genetic and physical interactors of BRD4, which converge on the folate pathway enzyme MTHFD1. W...
ORGANISM(S): Homo sapiens (Human) 
2019-04-04 | PXD012715 | Pride
Leukemia Mthfd1 Complex Brd4 
ProteomicsA heterochromatin specific RNA export pathway facilitates piRNA production
PIWI-interacting RNAs (piRNAs), a class of small RNAs that guide transposon silencing in animals, are processed in the cytoplasm from RNA Polymerase II transcripts. How piRNA precursors, which often lack RNA maturation signatures and thereby violate quality control checkpoints, achieve nuclear expor...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2019-03-29 | PXD012717 | Pride
Export Drosophila Ovary Pirna 
ProteomicsThe Fgf/Erf/NCoR1/2 repressive axis controls trophoblast cell fate
Here, we demonstrate that upon inhibition of the Fgf/Erk pathway in mouse trophoblast stem cells (TSCs), the Ets2 repressor factor (Erf) interacts with components of the Nuclear Receptor Corepressor Complex 1 and 2 (NCoR1 and NCoR2). Upon attenuation of Fgf signalling, unphosphorylated, nuclear Erf ...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-26 | PXD037892 | Pride
Trophoblast stem cells Erf Ncor2 Ncor1 
ProteomicsAutophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells
Somatic cells acclimate to changes in the environment by temporary reprogramming. Much has been learned about transcription factors that induce these cell-state switches in both plants and animals, but how cells rapidly modulate their proteome remains elusive. Here, we show rapid induction of autoph...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-12-04 | PXD016575 | Pride
Autophagy Arabidopsis Quantitative proteomics 
ProteomicsA cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress
We have identified a potential selective autophagy receptor protein in Arabidopsis thaliana, C53/AT5G06830/ERP1, that is recruited to ER upon ER-stress activation and induces autophagosome formation. By affinity proteomics IP/MS with ATG8A and E, we identified this new ER-phagy receptor, which is al...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-09-09 | PXD019988 | Pride
Arabidopsis thaliana Whole seedlings Tmt-11plex Tunicamycin Er-phagy Ip/ms Er-stress 
ProteomicsShuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and C53-mediated autophagy
UFMylation mediates the covalent modification of substrate proteins with UFM1 (Ubiquitin- fold modifier 1) and regulates the selective degradation of endoplasmic reticulum (ER) via autophagy (ER-phagy) to maintain ER homeostasis. Specifically, collisions of the ER-bound ribosomes trigger ribosome UF...
ORGANISM(S): Chlamydomonas reinhardtii 
2022-11-22 | PXD038157 | Pride
Chlamydomonas reinhardtii Rpl26 Er-phagy Ufmylation 
ProteomicsDifferential centrifugation coupled to affinity purification-mass spectrometry of mcherry-ATG8E
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been studied extensively, autophagosome maturation, i.e., delivery and fusion with the vacuole, remains largely unknown in plants....
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-10-18 | PXD031787 | Pride
Arabidopsis Lc-msms Autophagosomes Affinity purification 
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