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ProteomicsBrain adiponectin signaling controls peripheral insulin response in Drosophila
The brain plays a key role in energy homeostasis, detecting circulating hormones from peripheral organs, nutrients, and metabolites, and integrating this information to control food intake and energy expenditure. However, the signals mediating communication between peripheral organs and brain are la...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-06-15 | PXD013689 | Pride
Insulin response Energy homeostasis Growth Juvenile hormone Drosophila Adiponectin receptor 
ProteomicsEffect of xenosiderophores on Pseudomonas aeruginosa proteome
P. aeruginosa was described to sense xenosiderophores in the extracellular environment, leading to a regulation of genes encoding the siderophore uptake proteins. Data presented here allowed to characterize the proteomic response of P. aeruginosa in presence of different xenosiderophores. PAO1 strai...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2020-02-17 | PXD011950 | Pride
Pseudomonas Shotgun proteomics Xenosiderophores 
ProteomicsSpecific features and assembly of the plant mitochondrial complex I revealed by cryo-EM
Mitochondria are the powerhouses of eukaryotic cells and the site of essential metabolic reactions. Their main purpose is to maintain the high ATP/ADP ratio that is required to fuel the countless biochemical reactions taking place in eukaryotic cells1. This high ATP/ADP ratio is maintained through o...
ORGANISM(S): Brassica oleracea var. botrytis (Cauliflower) 
2020-10-26 | PXD017847 | Pride
Cryo-em Shotgun proteomics Complex i Mitochondria Plant 
ProteomicsSERRATE interacts with the nuclear exosome targeting complex to degrade primary miRNA precursors in Arabidopsis
0.3g of flowers were ground in liquid nitrogen. The powder was extracted for 30 min [H2] in 1.5 ml of lysis buffer (50mM Tris HCl pH 8.0, 50mM NaCl, 1% Triton x-100 and 1 x cOmplete™ EDTA-free protease inhibitor (Roche)). After removal of cell debris by centrifugation (2 times 10 min, 16000 x g, 4 ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-04-15 | PXD014447 | Pride
Exosome Pri-mirnas Next Serrate Mirna biogenesis 
ProteomicsThe esterase PfeE, Achilles heel in the battle for iron between Pseudomonas aeruginosa and Escherichia coli
Bacteria access iron, a key nutrient, by producing siderophores or using siderophores produced by other microorganisms. The pathogen Pseudomonas aeruginosa produces two siderophores but is also able to pirate enterobactin (ENT), the siderophore produced by Escherichia coli. ENT-Fe complexes are impo...
ORGANISM(S): Pseudomonas aeruginosa PAO1 Escherichia coli 
2021-04-29 | PXD023712 | Pride
Pseudomonas Pfee Siderophore Co-culture Iron 
ProteomicsProteomic analysis of DNA synthesis on a structured DNA template in human cellular extracts: Interplay between NHEJ and replication-associated proteins
It has been established that short inverted repeats (SIRs) trigger base substitution mutagenesis in human cells. However, how the replication machinery deals with this structured DNA is unknown. We have previously reported that in human cell-free extracts, DNA primer extension using a structured sin...
ORGANISM(S): Homo sapiens (Human) 
2020-02-04 | PXD011156 | Pride
Dna replication Short inverted repeats Nhej 
ProteomicsEscherichia coli killing by Shigella sonnei is mediated by colicins found in epidemiologically successful sublineages
Shigella sonnei as an enteric pathogen, can benefit from outcompeting gut commensals such as Escherichia coli and there can be several mechanisms that assist in interbacterial competition. It is, therefore, important to understand these mechanisms as they provide insights into the interactions of th...
ORGANISM(S): Shigella sonnei 
2023-10-24 | PXD036656 | Pride
Colicins Bper Shigella 
ProteomicsRACK1 interactome in drosophila S2 cells (FLAG-tag), using DDA and DIA (SWATH) approaches
Receptor for Activated C kinase 1 (RACK1) is a scaffold protein that has been found in association with several signaling complexes, and with the 40S subunit of the ribosome. Data presented here allowed a proteomic characterization of the interactome of RACK1 in drosophila S2 cells. We carried out L...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2017-05-29 | PXD002965 | Pride
Affinity purification Rna interference Rack1 Interactomics Drosophila 
ProteomicsArabidopsis mTERF9 protein promotes chloroplast ribosomal assembly and translation by establishing ribonucleoprotein interactions in vivo
The mitochondrial transcription termination factor proteins are nuclear-encoded nucleic acid binders defined by degenerate tandem helical-repeats of ~30 amino acids. They are found in metazoans and plants where they localize in organelles. In higher plants, the mTERF family comprises ~30 members and...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-12-15 | PXD018987 | Pride
Mterf Ribosome Rna Chloroplast 
ProteomicsHow to make a ribosome from mixed-up ribosomal RNA fragments in Chlamydomonas reinhardtii mitochondria
Mitochondria are essential components of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few, although essential, mitochondrial messenger RNAs still encoded by mitochondrial genomes. H...
ORGANISM(S): Chlamydomonas reinhardtii 
2022-03-15 | PXD024708 | Pride
Mitochondria   mitochondrial ribosome   mitoribosome   chlamydomonas reinhardtii   cryo-em   cryo-et   fragmented ribosomal rnas 
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